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Title Adipose tissue protocols / edited by Gérard Ailhaud
Published Totowa, N.J. : Humana Press, 2001


Description 1 online resource (xiii, 334 pages) : illustrations (some color)
Series Methods in molecular biology ; v. 155
Methods in molecular biology (Clifton, N.J.) ; v. 155
Contents Choosing an adipose tissue depot for sampling: factors in selection and depot specificity / Louis Casteilla -- Morphologic techniques for the study of brown adipose tissue and white adipose tissue / Saverio Cinti -- Confocal microscopy of adipocytes / Daniela Malide -- Cellularity measurements / Mario DiGirolamo -- Subcellular Fractionation of adipocytes and 3T3-L1 cells / Hans-Georg Joost -- Quantification of lipid-related mRNAs by reverse transcription-competitive polymerase chain reaction in human white adipose tissue biopsies / Hubert Vidal -- Glycogen synthase activity in adipose tissue: method for freeze-clamping and assay / Heidi K. Ortmeyer -- Assays of lipolytic enzymes / Cecilia Holm -- Assays of lipogenic enzymes / Raymond Bazin -- Assays of adrenergic receptors: including lipolysis and binding measurements / Christian Carpene -- Transfection of adipocytes and preparation of nuclear extracts / Isabelle Dugail -- Assay of membrane transport of long-chain fatty acids by adipocytes / Nada A. Abumrad -- Assays of glucose entry, glucose transporter amount, and translocation / Jean-Francois Tanti -- Methods to study phosphorylation and activation of the hormone-sensitive adipocyte phosphodiesterase type 3B in rat adipocytes / Eva Degerman -- Measurements of glucose conversion to its metabolites / Mario DiGirolamo -- Metabolite and ion fluxes and ion channels in brown and white adipocytes / Jan Nedergaard -- Culture of adipose tissue and isolated adipocytes / Susan K. Fried -- Cultures of adipose precursor cells from brown adipose tissue and of clonal brown adipocyte-like cell lines / Barbara Cannon -- Cultures of adipose precursor cells and cells of clonal lines form animal white adipose tissue / Raymond Negrel -- Cultures of human adipose presursor cells / Hans Hauner -- Measurements of peptide and nonpeptide secretory products from adipocytes / Marie-Christine Alessi -- Assessment of white adipose tissue metabolism by measurement of arteriovenous differences / Keith N. Frayn -- Measurement of adipose tissue blood flow / Jens Bülow -- Respiratory and thermogenic capacities of cells and mitochondria from brown and white adipose tissue / Barbara Cannon -- Measurements of white adipose tissue metabolism by the microdialysis tehcnique / Pierre Barbe -- Immunoassay measurements of leptin / Martin Bidlingmaier
Summary Adipose tissue is now recognized as a widely dispersed secretory organ that exhibits autocrine, paracrine, and endocrine properties, and plays a significant role in obesity, the most common health problem in industrialized countries. In Adipose Tissue Protocols, Gérard Ailhaud and a team of laboratory experts and clinicians describe in step-by-step detail the major techniques needed for the study of adipose tissue and cells. Drawn from both in vivo and in vitro studies, these readily reproducible methods cover a broad range of techniques, including the choice of adipose tissue depot and of morphological techniques for work on both brown adipose tissue (BAT) and white adipose tissue (WAT). Major treatment is accorded the isolation, subcellular fractionation, and transfection of low density adipocytes, as well as the metabolic aspects of nutrient uptake and key assays of nutrient and ion fluxes. Also covered are: biopsies and quantification of lipid-related mRNAs; cultures of adipose precursor cells from WAT and BAT; measurements of adipose secretory products; assessment of WAT metabolism in vivo; and assays of lipid-related enzymes. Innovative and highly practical, Adipose Tissue Protocols offers endocrinologists, physiologists, cell biologists, and pharmacologists a gold-standard collection of proven methods for effective nutritional, physiological, and molecular-level research on adipose tissue
Notes Source of title; title from title screen
Bibliography Includes bibliographical references and index
Issuing Body Digitization of Biomedprotocols made available on the world wide web by Humana Press
Notes English
Subject Adipose tissues -- Laboratory manuals
Adipose Tissue -- cytology
Adipose Tissue -- microbiology
Mycobacterium -- isolation & purification
Tissue Culture -- methods
SCIENCE -- Life Sciences -- Anatomy & Physiology.
Adipose tissues
Wetenschappelijke technieken.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Ailhaud, Gérard.
Humana Press.
ISBN 9781592592319
OTHER TI Biomedprotocols