Title Detection of pathogens in water using micro and nano-technology / [edited by] Giampaolo Zuccheri and Nikolaos Asproulis

Published London : IWA Publishing, 2012

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Description 1 online resource Contents Contents -- Preface -- Contributors -- Chapter 1: Overview of European regulation and standards on microbiological water analysis / Sabine Müller and Jonathan Loeffler -- 1.1. Introduction -- 1.2. European Regulation on Microbiological Analysis of Drinking Water -- 1.3. European Regulation on Microbiological Analysis of Recreational Water -- 1.4. European and International Standards for Microbiological Water Analysis -- Chapter 2: Risk analysis of bio-terroristic attacks on drinking water systems / Christian Mittermayr -- 2.1. Introduction -- 2.2. Definitions -- 2.3. Risk Analysis for Terrorism -- 2.4. Risk Analysis of Bio-Terroristic Attacks on Drinking Water Systems -- 2.5. Risk Estimation -- Chapter 3: Sample collection procedures for Online Contaminant Monitoring System / Miloslava Proksˇová, Marianna Cíchová and Lívia Tóthová-- 3.1. Introduction -- 3.2. Microbial Monitoring of Drinking Water -- 3.3. Sampling Plan -- 3.4. New Security Approaches for Drinking Water -- 3.5. New Approach of Online Contamination Monitoring Device (OCMD) -- 3.5.1. Location of OCMD in water system -- Chapter 4: A device to extract highly diluted specimens out of large volumes of water for analysis in lab-on-a-chip detection systems / Christoph Zeis -- 4.1. The Need for a Macro-to-Micro-Fluidic Interface -- 4.2. Principles of Separation -- 4.3. The Needle in the Haystack -- 4.4. Technical Description of the DINAMICS Concentration Apparatus -- 4.5. Conclusions -- Chapter 5: Sustainable DNA/RNA release methods for in-line waterborne pathogen screening devices / Hunor Sántha -- 5.1. Introduction -- 5.2. Survey on Pathogen-Lysis/Cell-Disruption Methods -- 5.3 Considerations for Water Samples and In-line (Quasi Continuous) operation -- 5.4 The Realised Case in The DINAMICS Project -- Chapter 6: The microsystem based core of the DINAMICS water testing system: Design considerations and realization of the chip units / Theo T. Veenstra -- 6.1 Microfluidics in DINAMICS -- 6.2 Layout of the Fluidic Parts of DINAMICS -- 6.3 Microfluidic Components in DINAMICS -- 6.4 Current Status -- 6.5 Conclusion -- Chapter 7: Electrochemical biosensor strategies for pathogen detection in water security / Daniele Gazzola, Simone Bonetti and Giampaolo Zuccheri -- 7.1 Introduction -- 7.2 Biosensor Strategies for Pathogen Detection in Water Security -- 7.3 Common Electrochemical Detection Systems for DNA Biosensors -- 7.4 The On-Chip Simplified Electrochemical Technique Developed in the DINAMICS Project -- Chapter 8: Biochemical and Nanotechnological Strategies for Signal Enhancement in the Detection of Nucleic Acids with Biosensors / Alessandra Vinelli, Manuele Onofri and Giampaolo Zuccheri8.1 Introduction -- 8.2 Enhancement Methods Based on Enzymatic (or Catalyzed) Reactions -- 8.3 Enhancement Methods Based on Nanoparticles or Nanostructures -- 8.4 Method Based on DNA Nanostructures -- 8.5 Conclusions and Perspectives -- Chapter 9: Computational modelling of aqueous environments in micro and nanochannels / D. Mantzalis, K. Karantonis, N. Asproulis, L. Könözsy and D. Drikakis -- 9.1 Introduction -- 9.2 Effects of Physical Characteristics -- 9.3 Computational Approaches -- 9.4 Liquid Flow in Confined Geometries -- Chapter 10: Computational recipes of transport phenomena in micro and nanofluidics / N. Asproulis10.1 Introduction -- 10.2 Modelling Approaches -- 10.3 Meta-Modelling for Macromolecules -- 10.4 Hybrid Continuum-Molecular Models -- References -- Chapter 11: Multi-detection of waterborne pathogens in raw and treated water samples by using ultrafiltration concentration and DNA array technology / Sophie Courtois, Anne Cajon, Aurore Romey, Fanny Poyet and Claude Mabilat -- 11.1 Introduction -- 11.2 Improved and Simplified Method for Concentrating Viral, Bacterial and Protozoan Pathogens -- 11.3 Integrated Protocol for Nucleic Acid Extraction, Amplification and Sequence Identification Through High Density Microarray -- 11.4 Results -- 11.5 Conclusions -- References -- Chapter 12: Detection and enumeration of waterborne mycobacteria / Joseph O. Falkinham III -- 12.1 Ecology of Waterborne Mycobacteria -- 12.2 Physiological Ecology of Waterborne Mycobacteria -- 12.3 Risk Analysis and Source-Tracking Environmental Mycobacteria -- 12.4 Sampling and Sample Treatment Strategies for Mycobacterial Detection and Enumeration -- 12.5 Mycobacterial Detection or Enumeration -- Chapter 13: New molecular technologies for the rapid detection of Legionella in water / E. Soria, M. A. Yáñez, R. Múrtula and V. Catalán -- 13.1 Introduction -- 13.2 Immunodetection and Legionella Fast Detection -- 13.3 Legionella Detection using Microfluidics -- 13.4 Future Research Directions -- Chapter 14: Detection of virus in the water environment / Johan Nordgren and Lennart Svensson -- 14.1 Introduction -- 14.2 Concentration of Virus from Water Samples -- 14.3 Detection and Quantification Methods -- 14.4 Perspectives -- References -- Chapter 15: Design of PCR primers for the detection of waterborne bacteria / Julien Gardès and Richard Christen -- 15.1 Introduction -- 15.2 The Target Genes -- 15.3 Design of PCR Primers -- 15.4 DNA-Based Detection Technologies -- 15.5 Conclusions -- References -- Chapter 16: Fluid structure and boundary slippage in nanoscale liquid films / Nikolai V. Priezjev -- 16.1 Abstract -- 16.2 Introduction -- 16.3 Molecular Dynamics Simulation Model -- 16.4 Results -- 16.5 Conclusions -- References -- Chapter 17: Understanding slip at the nanoscale in fluid flows using atomistic simulations / T. E. Karakasidis and A. Liakopoulos -- 17.1 Introduction – Definition of Slip -- 17.2 Importance of Slip -- 17.3 Experimental Measurement of Slip -- 17.4 Atomistic Simulations -- 17.5 Atomistic Simulations Results About Slip -- 17.6 Conclusions -- References Summary Detection of Pathogens in Water Using Micro and Nano-Technology aims to promote the uptake of innovative micro and nano-technological approaches towards the development of an integrated, cost-effective nano-biological sensor useful for security and environmental assays. The book describes the concerted efforts of a large European research project and the achievements of additional leading research groups. The reported knowledge and expertise should support in the innovation and integration of often separated unitary processes. Sampling, cell lysis and DNA/RNA extraction, DNA hybridisation detection micro-and nanosensors, microfluidics, together also with computational modelling and risk assessment can be integrated in the framework of the current and evolving European regulations and needs. The development and uptake of molecular methods is revolutionizing the field of waterborne pathogens detection, commonly performed with time-consuming cultural methods. The molecular detection methods are enabling the development of integrated instruments based on biosensor that will ultimately automate the full pathway of the microbiological analysis of water. EDITORS Giampaolo Zuccheri, University of Bologna, Italy Nikolaos Asproulis, Cranfield University, UK Contents: Overview of European regulation and standards on microbiological water analysis Risk analysis of bio-terroristic attacks on drinking water systems Sample collection procedures for Online Contaminant Monitoring System A device to extract highly diluted specimens out of large volumes of water for analysis in lab-on-a-chip detection systems Sustainable DNA/RNA release methods for in-line waterborne pathogen screening devices The microsystem based core of the DINAMICS water testing system: Design considerations and realization of the chip units Electrochemical biosensor strategies for pathogen detection in water security Biochemical and Nanotechnological Strategies for Signal Enhancement in the Detection of Nucleic Acids with biosensors Computational modelling of aqueous environments in micro and nanochannels Computational recipes of transport phenomena in micro and nanofluidics Multi-detection of waterborne pathogens in raw and treated water samples by using ultrafiltration concentration and DNA array technology Detection and enumeration of waterborne mycobacteria New molecular technologies for the rapid detection of Legionella in water Detection of virus in the water environment Design of PCR primers for the detection of waterborne bacteria Fluid structure and boundary slippage in nanoscale liquid films Understanding slip at the nanoscale in fluid flows using atomistic simulations Analysis Technology & Engineering Environmental General Science Applied Sciences Environmental Science (see Also Chemistry Environmental) Notes Electronic book text Epublication based on: 9781780401089 Bibliography Includes bibliographical references Subject Water -- Microbiology. Pathogenic microorganisms -- Detection. Nanotechnology. Water quality -- Measurement. Microtechnology. TECHNOLOGY & ENGINEERING -- Environmental -- Water Supply. SCIENCE -- Applied Sciences. Microtechnology Nanotechnology Pathogenic microorganisms -- Detection Water -- Microbiology Water quality -- Measurement Form Electronic book Author Zuccheri, Giampaolo. Asproulis, Nikolaos. ISBN 9781780401096 1780401094 1780401086 9781780401089

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