Title Biological microtechnique J.B. Sanderson

Published Oxford : BIOS Scientific Publishers in association with the Royal Microscopical Society, 1994

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Description 1 online resource Series Royal Microscopical Society microscopy handbooks ; 28 Microscopy handbooks ; 28. Contents Cover -- Half Title -- Title Page -- Copyright Page -- Preface -- Acknowledgements -- Table of contents -- Abbreviations -- Chapter 1: Introduction -- Collecting material for specimen preparation -- Choice of preparative technique -- Looking at preparations -- References -- Chapter 2: Fixation -- Function and use of fixatives -- Methods of fixation -- Immersion fixation -- Perfusion fixation -- Vapour fixation -- Phase-partition fixation -- Mechanical methods -- When not to use fixation -- The penetration of fixatives -- Osmolarity and pH -- Fixing agents -- Formaldehyde Glutaraldehyde and acrolein -- Alcohols and acetone -- Mercuric chloride -- Potassium dichromate -- Picric acid and acetic acid -- Osmium tetroxide -- Fixative mixtures -- Secondary fixation -- Preservatives -- Washing tissues -- Microwaves in histology -- Microwaving formalin-fixed tissues -- Leiden fixative -- Microwaving paraffin sections -- Microwaving cryostat sections -- Staining reactions -- Safety -- References -- Chapter 3: Tissue Processing -- Dehydration -- Transition media -- Processing schedules -- Automatic processing -- Embedding media -- Wax embedding -- Preparation for cutting Ribboning -- Ribboning difficulties -- Laying out ribbons -- Mounting sections onto slides -- The water-bath method -- The hot-plate method -- Marking slides -- Locating unstained sections -- Storing wax blocks -- Rehydrating sections -- Gelatin-based embedding media -- Other embedding media -- Polyethylene glycol (PEG) waxes -- Polyester waxes -- Ester wax -- Polyester wax -- Cellulose embedding -- Double embedding -- Orientation of small objects whilst embedding -- Resin embedding media -- Epoxy resins -- Acrylic resins -- Lowicryl and London resins -- Resin removal -- Methods for hard tissues Decalcification -- Determination of end-point -- Sectioning -- Wax impregnation -- Resin impregnation -- Lignified tissues -- Insect tissues -- Hair fibres -- Diatom frustules -- Recording tissue processing -- References -- Chapter 4: Microtomy -- Types of microtome -- Hand microtome -- Cambridge rocking microtome -- Rotary microtomes -- Base-sledge and sliding microtomes -- Freezing microtome -- Automated microtomes -- Clamps and chucks -- Types of knives -- Important knife angles and bevels -- Facets -- Care of knives -- Disposable blades -- Glass and diamond knives -- Cryotomy Cold knife methods -- Cryostats -- Freezing -- Embedding -- Hazards of cryogenic fluids -- Orientation of tissue -- Cryostat sectioning -- Sectioning temperatures -- Anti-roll plate -- Electrostatic charges -- Handling sections -- Storage of tissue -- Knife sharpening -- Lubricants -- Types of abrasive -- Handles and backs -- Manual sharpening -- Lapping -- Stropping -- Sectioning -- Wax structure -- Compression -- Clearance angle -- Sectioning technique -- Section thickness -- Cryostat sectioning -- Effect of fixation and processing on tissue size -- Sectioning difficulties -- Softening fluids Summary A completely new practical guide to both new and classical methods of slide-making which is easy-to-read and easy-to-understand. Biological Microtechnique contains a wealth of practical detail which will provide a firm grounding in preparative methods for light microscopy Analysis Biology Use of Microscopy Bibliography Includes bibliographical references and index Subject Microscopy -- Technique. Microtechnique. Biologie Microscopie -- méthode SCIENCE / Life Sciences / General Microbiología -- Técnicas de laboratorio. Microscopy -- Technique Microtechnique Form Electronic book Author Royal Microscopical Society (Great Britain) LC no. 95126451 ISBN 9781000102413 1000102416 9781003077008 1003077005 9781000119619 1000119610 9781000140941 1000140946

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