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Title Mouse genetics : methods and protocols / edited by Shree Ram Singh, Vincenzo Coppola
Published New York, NY : Humana Press, [2014]


Description 1 online resource (xiv, 475 pages) : illustrations (some color)
Series Methods in Molecular Biology, 1940-6029 ; Volume No. 1194
Methods in molecular biology (Clifton, N.J.) ; v. 1194. 1064-3745
Contents PART I. MOUSE GENETICS TECHNIQUES -- 1. Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre-loxP Site-Specific Recombination / Masato Ohtsuka -- 2. Generation of Conditional Knockout Mice / Kazuhito Sakamoto, Channabasavaiah B. Gurumurthy, and Kay-Uwe Wagner -- 3. Generating Mouse Lines for Lineage Tracing and Knockout Studies / Petra Kraus, V. Sivakamasundari, Xing Xing, and Thomas Lufkin -- 4. Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE) / Anton J.M. Roebroek and Bart Van Gool -- 5. Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection / Kun-Hsiung Lee -- 6. Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases / Susanne Feil, Jana Krauss, Martin Thunemann, and Robert Feil -- 7. Intracytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice / Pedro N. Moreira and Lluís Montoliu -- 8. Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice / Jane Beil and Thorsten Buch -- 9. Generation of Genetically Engineered Mice by the piggyBac Transposon System / Sheng Ding, Tian Xu, and Xiaohui Wu -- 10. Generation and Applications of MADM-Based Mouse Genetic Mosaic System / Hui Zong -- 11. Generation of Mouse Lines Conditionally Over-expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System / Claudia Piovan, Foued Amari, Francesca Lovat, Qun Chen, and Vincenzo Coppola -- 12. In Situ Hybridization (Both Radioactive and Nonradioactive) and Spatiotemporal Gene Expression Analysis / Olga Simmons, Esther M. Bolanis, Jian Wang, and Simon J. Conway
PART II. MOUSE GENETICS IN STEM CELLS -- 13. Isolation and Handling of Mouse Embryonic Fibroblasts / Kanika Jain, Paul J. Verma, and Jun Liu -- 14. Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue / Pollyanna Agnes Goh and Paul J. Verma -- 15. Pdx1 (GFP/w) Mice for Isolation, Characterization, and Differentiation of Pancreatic Progenitor Cells / Michael D. Williams, Wilson Wong, Amanda Rixon, Sarang N. Satoor, Anandwardhan A. Hardikar, and Mugdha V. Joglekar -- 16. Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development / Andrei M. Vacaru, Joseph Vitale, Johnathan Nieves, and Margaret H. Baron -- 17. Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System / Kristbjorn Orri Gudmundsson, Kevin Oakley, Yufen Han, and Yang Du -- 18. Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo / Lalit Sehgal, Abul Usmani, Sorab N. Dalal, and Subeer S. Majumdar
PART III. MOUSE GENETICS IN MODELING HUMAN DISEASE -- 19. Methods for the Detection of Genome Instability Derived from Replication Stress in Primary Mouse Embryonic Fibroblasts / Spencer W. Luebben, Naoko Shima, and Tsuyoshi Kawabata -- 20. Transgenic Nude Mice Ubiquitously Expressing Fluorescent Proteins for Color-Coded Imaging of the Tumor Microenvironment / Robert M. Hoffman -- 21. Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty / Viive M. Howell and Emily K. Colvin -- 22. Generating Double Knockout Mice to Model Genetic Intervention for Diabetic Cardiomyopathy in Humans / Vishalakshi Chavali, Shyam Sundar Nandi, Shree Ram Singh, and Paras Kumar Mishra -- 23. Experimental Osteoarthritis Models in Mice / Julia Lorenz and Susanne Grässel -- 24. Mouse Models and Methods for Studying Human Disease, Acute Kidney Injury (AKI) / Ganesan Ramesh and Punithavathi Ranganathan -- 25. Mouse Models of Acute and Chronic Colitis / Santhakumar Manicassamy and Indumathi Manoharan -- 26. Manipulation and Assessment of Gut Microbiome for Metabolic Studies / Sarang N. Satoor, Deepak P. Patil, Holly D. Kristensen, Mugdha V. Joglekar, Yogesh Shouche, and Anandwardhan A. Hardikar
Summary "Mouse Genetics: Methods and Protocols provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases ... each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods, and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls."--Publisher's description
Analysis biomedische wetenschappen
animal genetics
animal models
humane genetica
human genetics
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed August 6, 2014)
Subject Mice -- Genetic engineering -- Laboratory manuals
Mice -- Genetics -- Laboratory manuals
Mice -- Molecular genetics -- Laboratory manuals
Transgenic mice -- Laboratory manuals
Mice as laboratory animals -- Laboratory manuals
Animal models in research.
Genetics -- Technique.
Diseases -- Animal models.
Genetic Techniques
Mice, Transgenic -- genetics
Disease Models, Animal
Mice -- Genetic engineering
Mice -- Genetics
Mice -- Molecular genetics
Transgenic mice
Mice as laboratory animals
Animal models in research
Biology -- Laboratory manuals
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Singh, Shree Ram, editor
Coppola, Vincenzo, editor
ISBN 9781493912155