| Description |
1 online resource (xi, 291 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1738 |
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Methods in molecular biology (Clifton, N.J.) ; v. 1738. 1064-3745
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| Contents |
Metabolic profiling : status, challenges, and perspective / Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson -- Quality control and validation issues in LC-MS metabolomics / Olga Begou, Helen G. Gika, Georgios A. Theodoridis, and Ian D. Wilson -- Data treatment for LC-MS untargeted analysis / Samantha Riccadonna and Pietro Franceschi -- Bio- and chemoinformatics approaches for metabolomics data analysis / Michael Witting -- HILIC-MS/MS multi-targeted method for metabolomics applications / Christina Virgiliou, Helen G. Gika, and Georgios A. Theodoridis -- Ion pair chromatography for endogenous metabolites LC-MS analysis in tissue samples following targeted acquisition / Filippos Michopoulos -- LC-MS untargeted analysis / Elizabeth J. Want -- NMR-based metabolic profiling procedures for biofluids and cell and tissue extracts / Dimitra Benaki and Emmanuel Mikros -- Untargeted GC-MS metabolomics / Matthaios-Emmanouil P. Papadimitropoulos, Catherine G. Vasilopoulou, Christoniki Maga-Nteve, and Maria I. Klapa -- Rat fecal metabolomics-based analysis / Olga Deda, Helen G. Gika, and Georgios A. Theodoridis -- GC-MS metabolomic profiling of protic metabolites following heptafluorobutyl chloroformate mediated dispersive liquid microextraction sample preparation protocol / Petr Hušek, Zdeněk Švagera, Dagmar Hanzlíková, Iva Karlínová, Lucie Řimnáčová, Helena Zahradníčková, and Petr Šimek -- Sheathless capillary electrophoresis-mass spectrometry for the profiling of charged metabolites in biological samples / Rawi Ramautar -- Two-phase extraction for comprehensive analysis of the plant metabolome by NMR / Jan Schripsema and Denise Dagnino -- NMR spectroscopy protocols for food metabolomics applications / Evangelia Ralli, Maria Amargianitaki, Efi Manolopoulou, Maria Misiak, Georgios Markakis, Sofia Tachtalidou, Alexandra Kolesnikova, Photis Dais, and Apostolos Spyros -- Direct injection analysis of fruit VOCs by PTR-ToF-MS : the apple case study / Brian Farneti -- LC-MS untargeted protocol for the analysis of wine / Panagiotis Arapitsas and Fulvio Mattivi -- Tissue multiplatform-based metabolomics/metabonomics for enhanced metabolome coverage / Panagiotis A. Vorkas, M.R. Abellona U, and Jia V. Li -- UHPLC-HRMS analysis for steroid profiling in serum (steroidomics) / Federico Ponzetto, Julien Boccard, Raul Nicoli, Tiia Kuuranne, Martial Saugy, and Serge Rudaz -- Metabolomics in human acute-exercise trials : study design and preparation / Aikaterina Siopi and Vassilis Mougios |
| Summary |
Annotation This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling. This book is divided into IV parts. Part I contains chapters that highlight basic concepts, such as experimental design, data treatment, metabolite identification, and harmonization. Part II describes experimental protocols for both targeted and untargeted metabolomics covering the basic analytical technologies: LC-MS, GC-MS, NMR and CE-MS. In addition the protocols describe methods for the study of tissues, feces, blood and other types of biological samples as well as the application of chemical derivatization for GC-MS. Parts III and IV present the use of metabolomics in the study of food, plants and the life sciences, with examples from the quest for the discovery of disease biomarkers, physical exercise omics and metabolic profiling of food, fruit and wine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Metabolic Profiling: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this rapidly developing field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed April 16, 2018) |
| Subject |
Metabolism -- Testing -- Laboratory manuals
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Metabolomics -- methods
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Metabolome
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Mass Spectrometry -- methods
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Chromatography, Liquid -- methods
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Magnetic Resonance Spectroscopy -- methods
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Biochemistry.
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Science -- Life Sciences -- Biochemistry.
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Metabolism -- Testing
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Theodoridis, Georgios A., editor
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Gika, Helen G., editor
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Wilson, Ian D., editor
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| ISBN |
9781493976430 |
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1493976435 |
|
1493976427 |
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9781493976423 |
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