| Description |
1 online resource |
| Contents |
Intro -- Applied Environmental Metabolomics: Community insights and guidance from the field -- Copyright -- Contents -- Contributors -- Foreword -- Part 1 Introduction -- Chapter 1 Applied environmental metabolomics: Eliciting viewpoints from the metabolomics research community -- Introduction -- Reports from the field -- Case study contributions -- The future -- References -- Part 2 Reports from the field -- Chapter 2 Measuring root exudate metabolites in holm oak (Quercus ilex) under drought and recovery☆ -- Scope -- Aims and objectives -- Introduction -- Approach (materials and methods) -- Biological sample -- Environment sampled -- Exudate sampling protocol -- Main findings -- Summary of project outcomes -- Concerns and recommendations -- Summary and future perspectives -- References -- Chapter 3 Visualization of cyanogenic glycosides in floral tissues☆ -- Scope -- Aims and objectives of the study -- Introduction -- Approach (material and methods) -- Biological samples -- Sampling protocol -- Analytical protocol -- Quantitative determination of cyanogenesis -- Extraction and identification of cyanogenic glycosides -- MALDI mass spectrometry imaging -- Main findings -- Quantification of floral cyanogenic glycoside concentrations -- Identification of cyanogenic glycosides -- Detection of cyanogenic glycosides in Proteaceae flowers using MALDI-MSI -- What went right -- What went wrong -- Suggestions for improvements for future studies -- Final remarks -- Acknowledgments -- References -- Chapter 4 Environmental assessment of metal impacted soils using community metabolic profiling ☆ -- Scope -- Aims and objectives -- Introduction -- Approach (materials and methods) -- Site description -- Soil analysis -- Metabolite extraction -- 1 H NMR analysis -- GC-MS analysis -- Statistical analysis -- Main findings -- Soil analysis -- 1 H Nmr -- Gc-Ms |
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Multivariate analysis -- Discussion -- What went right? -- What could be improved? -- Labour intensive sample analysis -- Sample matrix -- Metabolite ID -- Integration -- Final thoughts -- References -- Chapter 5 Decoding the metabolic landscape of maize responses to experimentally controlled drought stress: A greenhouse case -- Scope -- Aims and objectives -- Introduction -- Approach (material and methods) -- Biological samples: Plant cultivation and treatments -- Sampling protocol: Phenotypic and physiological evaluation, harvesting plant materials and metabolite extraction -- Analytical protocol -- Metabolomics data acquisition: LC-MS-based untargeted and targeted analyses -- Data mining: Data processing and chemometrics -- Metabolite annotation for untargeted metabolomics data -- Main findings -- Metabolic responses to drought: Dynamic changes in both primary and secondary metabolism -- Global reprogramming of the maize metabolism in response to drought stress spans several metabolic pathways -- Concluding remarks-"What went right" and "what went wrong" -- Acknowledgments -- Conflict of interest -- References -- Chapter 6 Nontargeted screening of metabolites to discriminate disease suppressive and nonsuppressive soils for the fungal ... -- Scope -- Aims -- Introduction -- Approach -- Biological sample -- Soil sampling protocol -- Environment sampled -- Analytical protocol -- Main findings -- What went right? -- Site selection -- Soil sampling strategy -- Pilot studies -- What went wrong? -- Different cereal crops sown in the suppressive and nonsuppressive fields -- Lack of suitable databases for annotation -- Topography -- Recommendations for agricultural field studies -- Final remarks -- References -- Chapter 7 Ecosystem metabolomics of dissolved organic matter from arctic soil pore water across seasonal transitions ☆ -- Scope |
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Aims and objectives -- Introduction -- Approach -- Environment sampled -- Sampling protocol -- Analytical protocol -- Main findings -- Biogeochemical variables -- Soil porewater metabolome -- What went right -- What went wrong -- Conclusion -- References -- Chapter 8 Temporal trends in metabolite profiles correspond with seasonal patterns of temperature and rainfall during field... -- Scope -- Aims and objectives of the study -- Introduction -- Approach (materials and methods) -- Biological samples -- Environment sampled -- Sampling protocol -- Analytical protocol -- Main findings -- What went right? -- What would you recommend to other researchers wanting to do similar research? -- What could be improved? -- Final thoughts -- References -- Chapter 9 Metabolic responses of eelgrass (Zostera marina) to artificially induced stresses☆ -- Scope -- Aims and objectives -- Introduction -- Approach (material and methods) -- Biological samples -- Environment sampled -- Sampling protocol -- Herbicide exposure -- Low light (dark) and high temperature -- Growth, photosynthetic efficiency -- Analytical protocol -- Main findings -- Physiological effects on eelgrass -- Metabolic fluctuations associated with ambient stressors -- What went right -- What went wrong -- Summary and future perspectives -- References -- Chapter 10 Metabolomic profiling of anthropogenically threatened Australian seagrass Zostera muelleri using one- and two-di... -- Scope -- Aims and objectives -- Introduction -- Experimental methods -- Seagrass sample collection -- Sample preparation -- Metabolite profiling -- Data preprocess and analysis -- Main findings -- What went wrong in seagrass metabolomics -- Leaf age and lifespan influence metabolite profiling -- Need for marine plants-based MS libraries -- What went right in seagrass metabolomics -- Detection of specific classes of compounds |
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Detection of phenylpropenoid compounds and aromatic amino acids -- Detection of unique sugars, their derivatives, and phytohormones -- Summary and future perspectives -- Acknowledgment -- References -- Chapter 11 The metabolic response of marine copepods (Calanus spp.) to food deprivation, end-of-century ocean acidificatio... -- Scope -- Aims and objectives of the study -- Introduction -- Approach (material and methods) -- Biological sample -- Environment sampled -- Sampling protocol -- Analytical protocol -- Main findings -- What went right? -- What went wrong? -- Summary and future perspectives -- Acknowledgments -- References -- Further reading -- Chapter 12 Untargeted screening of xenobiotics and metabolic profiles of green sea turtles on the Great Barrier Reef☆ -- Scope -- Aims and objectives -- Introduction -- Approach (material and methods) -- Biological sample -- Environment sampled -- Sampling protocol -- Analytical protocol -- Main findings -- Evidence of oxidative stress in Upstart Bay turtles -- Evidence of anthropogenic influence in Cleveland Bay -- What went right -- Unique biological samples -- Data-rich regional profile -- Case-control sampling strategy -- What went wrong -- Labor-intensive data analysis -- Noncomprehensive sample and data analysis -- Delayed response to catastrophic event -- Suggestions for future studies -- Summary and future perspectives -- References -- Chapter 13 Butterflyfish gill mucus metabolome reflects diet preferences and gill parasite intensities☆ -- Scope -- Aims and objectives -- Introduction -- Approach (materials and methods) -- Biological sample -- Environment sampled -- Sampling protocol -- Analytical protocol -- Main findings -- What went right -- Large collection of wild biological samples -- Metabolomic pipeline -- What went wrong -- Samples limitations -- Limited feature annotation |
| Summary |
And future perspectives -- References -- Chapter 14 Exploring the coral bleaching tipping point with 13 C metabolomics ☆ -- Scope -- Aims and objectives -- Introduction -- Approach (material and methods) -- Biological sample -- Environment sampled -- Sampling protocol -- Analytical protocol -- Main findings -- Thermal stress indicators -- 13 C enrichment of free metabolite pools -- Symbiont thermal stress -- Host thermal stress -- What went well -- What went wrong -- What data did you wish you had but did not? -- Summary and future perspectives -- References -- Chapter 15 The metabolic significance of symbiont community composition in the coral-algal symbiosis☆ -- Scope -- Aims and objectives -- Introduction -- Approach (materials and methods) -- Biological sample -- Environment sampled -- Sampling protocol -- Analytical protocol -- Main findings -- What went right? -- Rapid in situ sample collection -- Successful separation of host and symbiont -- First metabolite comparison of natural variations in symbiont community -- What went wrong? -- Small and unequal sample sizes -- Measurement of additional water quality measures -- Spatial and temporal variation -- Noncomprehensive information on host genotype and complete Symbiodiniaceae community composition -- Symbiodiniaceae physiology -- Summary and future perspectives -- References -- Chapter 16 Evaluating the effects of environmental perturbations in bloom forming cyanobacteria through untargeted metabolomi -- Scope -- Aims and objectives -- Introduction -- Approach (materials and methods) -- Biological sample -- Sampling protocol -- Extraction and analytical protocols -- Main findings -- What went right -- Permits came in timely -- Time-points for mesocosm experiments -- Quality of samples from mesocosm experiments -- Database availability for model cyanobacteria |
| Notes |
Description based on online resource; title from digital title page (viewed on July 18, 2022) |
| Subject |
Metabolites -- Environmental aspects
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Metabolism -- Environmental aspects
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Ecology.
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Ecology
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Metabolites -- Environmental aspects
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| Form |
Electronic book
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| Author |
Beale, David J. (David John)
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| ISBN |
0128164611 |
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9780128164617 |
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