| Description |
xx, 165 pages : illustrations ; 24 cm |
| Series |
Springer lab manual |
|
Springer lab manual.
|
| Contents |
Pt. 1. Theoretical and Methodical Prerequisites for Using PCR to Quantitate Nucleic Acids. Ch. 1.1. General Aspects and Chances of Nucleic Acid Quantitation by PCR / Th. Kohler. Ch. 1.2. Design of Suitable Primers and Competitor Fragments for Quantitative PCR / Th. Kohler. Ch. 1.3. Cloning of Short DNA Fragments and In Vitro Transcription to Generate RNA Standards / D. Lassner. Ch. 1.4. Direct Non-Isotopic Sequencing of PCR Products or Standards /B. Thamm -- Pt. 2. Conventional Techniques for mRNA Analysis. Ch. 2.1. Isolation of mRNA / D. Lassner. Ch. 2.2. Synthesis of cDNA / D. Lassner. Ch. 2.3. Qualitative RT-PCR: Amplification of Synthesized mdr-1 cDNA / Th. Kohler. Ch. 2.4. Single-Tube RT-PCR / D. Lassner. Ch. 2.5. Nonradioactive Determination of PCR Products by Using a DIG-Labeled DNA Probe (Dot Blot) /Th. Kohler. Ch. 2.6. Nonradioactive Northern Blot Hybridization with DIG-Labeled DNA Probes / A.-K. Rost |
|
Pt. 3. Semiquantitative and Quantitative Protocols for Measurement of Nucleic Acids by PCR. Ch. 3.1. Quantitation of mRNA by the ELOSA Technique Using External Standards / D. Lassner. Ch. 3.2. Semiquantitative Detection of Viral DNA, e.g. for CMV, by Using the DNA Enzyme Immunoassay (DEIA) / B. Pustowoit. Ch. 3.3. HPLC - Analysis of Nucleic Acids /H. Remke and Th. Kohler. Ch. 3.4. Quantitation of Absolute Numbers of mRNA Copies in a cDNA Sample by Competitive PCR / Th. Kohler |
| Bibliography |
Includes bibliographical references |
| Subject |
Messenger RNA -- Analysis -- Laboratory manuals.
|
|
Polymerase chain reaction -- Laboratory manuals.
|
|
RNA, Messenger -- analysis.
|
|
Polymerase Chain Reaction.
|
| Genre/Form |
Laboratory Manual.
|
|
Laboratory manuals.
|
| Author |
Köhler, Th. (Thomas), 1959-
|
| LC no. |
95035772 |
| ISBN |
3540591923 (softcover : alk. paper) |
|
