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Title Adult stem cells : methods and protocols / edited by Paolo Di Nardo, Sanjiv Dhingra, Dinender K. Singla
Published New York : Humana Press : Springer, [2017]


Description 1 online resource (xii, 324 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; 1553
Methods in molecular biology (Clifton, N.J.) ; v. 1553. 1064-3745
Contents Simple protocol to isolate, characterize, and expand dental pulp stem cells / Federica Di Scipio, Andrea Elio Sprio, Maria Elisabetta Carere, Zhiqian Yang, and Giovanni Nicolao Berta -- Derivation of mesenchymal stem cells from embryonic stem cells : a non-variable and inexhaustive source of adult stem cells / Glen Lester Sequiera, Niketa Sareen, Ejlal Abu El-Rub, and Sanjiv Dhingra -- Compact bone-derived multipotent mesenchymal stromal cells (MSCs) for the Tteatment of Sjogren's-like disease in NOD mice / Ghada Abu Elghanam, Younan Liu, Saeed Khalili, Dongdong Fang, and Simon D. Tran -- Manipulating the proliferative potential of cardiomyocytes by gene transfer / Giulia Prosdocimo and Mauro Giacca -- Assessment of energy metabolic changes in adipose tissue-derived stem cells / Ghazaleh Hajmousa and Martin C. Harmsen -- Isolation and in vitro characterization of epidermal stem cells / Kasper S. Moestrup, Marianne S. Andersen, and Kim B. Jensen -- Endothelial progenitor cells : procedure for cell isolation and applications / Garikipati V.N. Srikanth and Raj Kishore -- Therapeutic application of placental mesenchymal stem cells reprogrammed neurospheres in spinal cord injury of SCID / Vikram Sabapathy, Franklin Jebaraj Herbert, and Sanjay Kumar -- Therapeutic application of human wharton jelly mesenchymal stem cells in skin injury of SCID / Vikram Sabapathy, Balasubramanian Sundaram, and Sanjay Kumar -- Isolation, characterization, and expansion of cancer stem cells / Luke A. Torre-Healy, Artem Berezovsky, and Justin D. Lathia -- Cardiac progenitor cell extraction from human auricles / Paolo Di Nardo and Francesca Pagliari -- Isolation and culture of satellite cells from mouse skeletal muscle / Antonio Musarò and Silvia Carosio -- Isolation of stromal stem cells from adipose tissue / Maria Prat, Francesca Oltolina, Silvia Antonini, and Andrea Zamperone -- Human-induced pluripotent stem cell-derived mesenchymal stem cells as an individual-specific and renewable source of adult stem cells / Glen Lester Sequiera, Sekaran Saravanan, and Sanjiv Dhingra -- Simplified and systematic method to isolate, culture, and characterize multiple types of human dental stem cells from a single tooth / Mohammed Bakkar, Younan Liu, Dongdong Fang, Camille Stegen, Xinyun Su, Murali Ramamoorthi, Li-Chieh Lin, Takako Kawasaki, Nicholas Makhoul, Huan Pham, Yoshinori Sumita, and Simon D. Tran -- Measurement of autophagy by flow cytometry / Silvia Zappavigna, Angela Lombardi, Gabriella Misso, Anna Grimaldi, and Michele Caraglia -- Echocardiography-guided intramyocardial injection method in a murine model / Kay Maeda, Rick Seymour, Marc Ruel, and Erik J. Suuronen -- Noninvasive assessment of cell fate and biology in transplanted mesenchymal stem cells / Federico Franchi and Martin Rodriguez-Porcel -- Methods for long-term storage of murine bone marrow-derived mesenchymal stem cells / Niketa Sareen, Ejlal Abu-El-Rub, Glen Lester Sequiera, Meenal Moudgil, and Sanjiv Dhingra -- Therapeutic application of adult stem cells in the heart / Taylor A. Johnson and Dinender K. Singla -- Patient-derived and intraoperatively formed biomaterial for tissue engineering / Shalmli U. Joshi, Rares O. Barbu, Melissa Carr-Reynolds, Brian Barnes, and Syam P. Nukavarapu -- Three-dimensional printed scaffolds with multipotent mesenchymal stromal cells for rabbit mandibular reconstruction and engineering / Dongdong Fang, Michael Roskies, Mohamed-Nur Abdallah, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin, Faleh Tamimi, and Simon D. Tran -- Optimal environmental stiffness for stem cell mediated ischemic myocardium repair / Honghai Liu, Christian Paul, and Meifeng Xu -- Assessment of MiRNA regulation of endothelial progenitor cell mediated angiogenesis / Darukeshwara Joladarashi and Prasanna Krishnamurthy -- Stem cell exosomes : cell-freetherapy for organ repair / Mohsin Khan and Raj Kishore
Summary This volume contains a collection of protocols from some of the major laboratories involved in stem cell research across the world. The research discussed in this book covers topics such as: isolating, characterizing and expanding dental pulp stem cells; manipulating the proliferative potential of cardiomyocytes by gene transfer; isolation of stromal stem cells from adipose tissue; noninvasive assessment of cell fat and biology in transplanted mesenchymal stem cells; and cell-free therapy for organ repair. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Adult Stem Cells: Methods and Protocols is a valuable resource for helping researchers transform the study of stem cells into an industrialized process that will supply patients with efficient, safe, and cost-effective cell treatments
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF of title page (Springer Protocols, viewed February 28, 2017)
Subject Stem cells -- Laboratory manuals
Cell culture -- Laboratory manuals
Cell culture.
Cytology -- Technique.
Adult Stem Cells -- cytology
Mesenchymal Stem Cells -- cytology
Cell Culture Techniques
Cells, Cultured
Cytological Techniques
Cellular biology (cytology).
Science -- Life Sciences -- Biology -- Molecular Biology.
Cytology -- Technique
Cell culture
Stem cells
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Di Nardo, Paolo, editor
Singla, Dinender K., editor
Dhingra, Sanjiv, editor
LC no. 2016963441
ISBN 9781493967568