Title Membrane trafficking / edited by Ales Vancura

Published Totowa, N.J. : Humana Press, ©2008

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Description 1 online resource (xv, 405 pages) : illustrations Series Methods in molecular biology ; 457 Methods in molecular biology (Clifton, N.J.) ; v. 457. Contents pt. I. Yeast Cells -- Ch. 1. Freight management in the cell / Johannes M. Herrmann, Anne Spang -- Ch. 2. High-throughput protein extraction and immunoblotting analysis in saccharomyces cerevisiae / Todd C. Lorenz, Vikram C. Anand, Gregory S. Payne -- Ch. 3. Genome-wide analysis of membrane transport using yeast knockout arrays / Helen E. Burston, Michael Davey, Elizabeth Conibear -- Ch. 4. A cell-free system for reconstitution of transport between prevacuolar compartments and vacuoles in saccharomyces cerevisiae / Thomas A. Vida -- Ch. 5. In vitro analysis of the mitochondrial preprotein import machinery using recombinant precursor polypeptides / Dorothea Becker, Martin Krayl, Wolfgang Voos -- Ch. 6. In vitro import of proteins into isolated mitochondria / Karl Bihlmaier, Melanie Bien, Johannes M. Herrmann -- Ch. 7. Synthesis and sorting of mitochondrial translation products / Heike Bauerschmitt, Soledad Funes, Johannes M. Herrmann -- Ch. 8. In vivo labeling and analysis of mitochondrial translation products in budding and in fission yeasts / Karine Gouget, Fulvia Verde, Antoni Barrientos Ch. 9. Exploring protein-protein interactions involving newly synthesized mitochondrial DNA encoded proteins / Darryl Horn, Flavia Fontanesi, Antoni Barrientos -- Ch. 10. Purification of yeast membranes and organelles by sucrose density gradient centrifugation / Jennifer Chang, Victoria Ruiz, Ales Vancura -- Ch. 11. Microscopic analysis of lipid droplet metabolism and dynamics in yeast / Heimo Wolinski, Sepp D. Kohlwein -- Ch. 12. Use of bimolecular fluorescence complementation (BiFC) in yeast saccharomyces cerevisiae / Kari-Pekka Skarp [and others] -- pt. II. Mammalian cells -- Ch. 13. Using quantitative fluorescence microscopy to probe organelle assembly and membrane trafficking / Brian Storrie, Tregei Starr, Kimberly Forsten-Williams -- Ch. 14. Measuring secretory membrane traffic / Vytaute Starkuviene [and others] -- Ch. 15. A correlative light and electron microscopy method based on laser micropatterning and etching / Julien Colombelli [and others] -- Ch. 16. Approaches to investigate the role of signaling in ER-to-Golgi transport / Laura J. Sharpe, Ximing Du, Andrew J. Brown -- Ch. 17. Recruitment of coat proteins to peptidoliposomes / Gregor Suri, Martin Spiess, Pascal Crottet Ch. 18. SNAREs mediated fusion of liposomes / Jérôme Vicogne, Jeffrey E. Pessin -- Ch. 19. Use of polarized PC12 cells to monitor protein localization in the early biosynthetic pathway / Ragna Sannerud [and others] -- Ch. 20. Tracking the transport of E-cadherin to and from the plasma membrane / Matthew P. Wagoner, Kun Ling, Richard A. Anderson -- Ch. 21. Analysis of nucleocytoplasmic shuttling of NFkB proteins in human leukocytes / Chandra C. Ghosh [and others] -- Ch. 22. Imaging pHluorin-based probes at hippocampal synapses / Stephen J. Royle [and others] -- Ch. 23. Analysis of receptor tyrosine kinase internalization using flow cytometry / Ning Li, Kristen S. Hill, Lisa A. Elferink -- Ch. 24. Measurement of receptor endocytosis and recycling / Jane M. Knisely, Jiyeon Lee, Guojun Bu -- Ch. 25. Styryl dye-based synaptic vesicle recycling assay in cultured cerebellar granule neurons / Victor Anggono, Michael A. Cousin, Phillip J. Robinson -- Ch. 26. Use of quantitative immunofluorescence microscopy to study intracellular trafficking / Vincent Blot, Timothy E. McGraw -- Ch. 27. Dissecting GLUT4 traffic components in L6 myocytes by fluorescence-based, single cell assays / Costin N. Antonescu, Varinder K Randhawa, Amira Klip -- Ch. 28. Functional genetic analysis of the mammalian mitochondrial DNA encoded peptides / Maria Pilar Bayona-Bafaluy [and others] Summary As membrane trafficking research has expanded over the past thirty years, a remarkable convergence of information has been gained by using genetic approaches in yeast cells with biochemical approaches in mammalian cells. Membrane Trafficking reflects these advances by devoting one section of the book to yeast cells and the other to mammalian cells, with each section providing both classic and cutting-edge techniques to study macromolecular transport across the membranes. These protocols, contributed by top experts in the field, have been tested in laboratories all around world. As a volume of the highly successful Methods in Molecular Biology series, the chapters include step-by-step laboratory protocols, a list of necessary materials and reagents, and the greatly beneficial Notes section, which aids in troubleshooting and avoiding known pitfalls. Comprehensive and state-of-the-art, Membrane Trafficking is the ideal reference for both senior researchers experienced in the field and graduate students studying membrane trafficking for the first time Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL English Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Subject Biological transport. Cell membranes. Cytology -- Technique. Physiology. Carrier proteins. Membrane proteins. Life sciences. Metabolism. Medical laboratory technology. Biology. Proteins. Physical sciences. Cells. Anatomy. Cell Membrane Membrane Transport Proteins Biological Transport Protein Transport Laboratory Manuals Cytological Techniques Genetics Physiology Carrier Proteins Membrane Proteins Cellular Structures Biological Science Disciplines Metabolism Clinical Laboratory Techniques Publication Formats Biology Proteins Investigative Techniques Publication Characteristics Natural Science Disciplines Cells Anatomy Analytical, Diagnostic and Therapeutic Techniques and Equipment Amino Acids, Peptides, and Proteins Phenomena and Processes Disciplines and Occupations Chemicals and Drugs Animal Structures physiology. biological sciences. metabolism (biological concept) biology. protein. physical sciences. anatomy. Proteins Physiology Physical sciences Metabolism Membrane proteins Medical laboratory technology Life sciences Cytology -- Technique Cells Cell membranes Carrier proteins Biology Anatomy Biological transport Membrantransport Hefeartige Pilze Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Vancura, Ales ISBN 9781597452618 1597452610

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