| Description |
1 online resource (xiv, 423 pages) : illustrations |
| Series |
Methods in molecular biology, 1940-6029 ; 2127 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 2127. 1064-3745
|
| Contents |
Cloning and Multi-Subunit Expression of Mitochondrial Membrane Protein Complexes in Saccharomyces cerevisiae -- Membrane Protein Production in Escherichia coli -- Membrane Protein Production in Lactococcus Lactis for Structural Studies -- Expression and Purification of Membrane Proteins in Saccharomyces cerevisiae -- Membrane Protein Expression in Insect Cells Using the Baculovirus Expression Vector System -- Membrane Extracts from Plant Tissues -- Membrane Protein Solubilization and Quality Control: An Example of a Primary Active Transporter -- GPCR Solubilization and Quality Control -- Affinity Purification of Membrane Proteins -- Purification of Membrane Proteins by Affinity Chromatography with On-Column Protease Cleavage -- Biotinylation of Membrane Proteins for Binder Selections -- Production and Application of Nanobodies for Membrane Protein Structural Biology -- Identifying Conformation-Selective Heavy-Chain-Only Antibodies against Membrane Proteins by a Thermal-Shift Scintillation Proximity Assay -- Reconstitution of Membrane Proteins into Platforms Suitable for Biophysical and Structural Analyses -- Reconstitution of Proteoliposomes for Phospholipid Scrambling and Non-Selective Channel Assays -- Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent -- Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs -- Fast Small-Scale Membrane Protein Purification and Grids Preparation for Single Particle Electron Microscopy -- Stabilization and Crystallization of a Membrane Protein Involved in Lipids Transport -- In Meso In Situ Serial X-ray Crystallography (IMISX): A Protocol for Membrane Protein Structure Determination at the Swiss Light Source -- Membrane Protein Preparation for Serial Crystallography Using High Viscosity Injectors: Rhodopsin as an Example -- Hydrogen/Deuterium Exchange Mass Spectrometry for the Structural Analysis of Detergent-Solubilized Membrane Proteins -- Mechanical Unfolding and Refolding of Single Membrane Proteins by Atomic Force Microscopy -- Sample Preparation and Technical Setup for NMR Spectroscopy with Integral Membrane Proteins -- Exploring Lipid- and Membrane Protein-Dynamics Using Lipid-Bilayer Nanodiscs and Solution-State NMR Spectroscopy |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed January 25, 2021) |
| Subject |
Membrane proteins -- Laboratory manuals
|
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Membrane Proteins -- physiology
|
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Membrane Proteins -- isolation & purification
|
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Membrane proteins.
|
| Genre/Form |
Laboratory manuals.
|
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Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Perez, Camilo, editor
|
|
Maier, Timm, editor
|
| ISBN |
9781071603734 |
|
1071603736 |
|
1071603728 |
|
9781071603727 |
|
