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Title Biomembrane protocols. I, Isolation and analysis / edited by John M. Graham, Joan A. Higgins
Published Totowa, N.J. : Humana Press, 1993

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Description 1 online resource (300 x, 313 pages) : illustrations
Series Methods in molecular biology ; v. 19
Methods in molecular biology (Clifton, N.J.) ; v. 19.
Contents Machine generated contents note: CH. 1 Crystallization of Membrane Proteins for X-Ray Analysis / Brian J. Sutton / Maninder K. Sohi -- CH. 2 Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination / William A. Muller -- CH. 3 Biochemical Methods to Determine Cell-Surface Topography / William A. Muller -- CH. 4 Use of Antipeptide Antibodies for the Isolation and Study of Membrane Proteins / Stephen A. Baldwin -- CH. 5 Production of Monoclonal Antibodies to Membrane Proteins / Lynda J. Partridge -- CH. 6 Purification of a Membrane Protein (Ca[superscript 2+]/Mg[superscript 2+]-ATPase) and Its Reconstitution into Lipid Vesicles / J. Malcolm East -- CH. 7 Measurement of Protein-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy / Anthony G. Lee -- CH. 8 Measurement of Lipid-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy / Anthony G. Lee -- CH. 9 Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as Probes / Joan A. Higgins -- CH. 10 Determination of the Transverse Topography of Membrane Phospholipids Using Phospholipid Transfer Proteins as Tools / Joan A. Higgins -- CH. 11 Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry / Robert F.A. Zwaal / Paul Comfurius / Edouard Bevers -- CH. 12 Fluorescent Glycerolipid Probes: Synthesis and Use for Examining Intracellular Lipid Trafficking / Richard G. Sleight -- CH. 13 Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids / Alain Zachowski / Pierre Fellmann / Philippe F. Devaux -- CH. 14 Measurement of Membrane Fluidity and Membrane Fusion with Fluorescent Probes / C. Lindsay Bashford -- CH. 15 Extraction and Assay of Cyclic Nucleotides / Guy St. J. Whitley
Note continued: CH. 16 Analysis of G-Proteins Regulating Signal Transduction Pathways / Margaret M. Harnett -- CH. 17 Assay of Protein Kinases and Protein Phosphorylation / Margaret M. Harnett -- CH. 18 Analysis of Cellular Phosphoinositides and Phosphoinositols by Extraction and Simple Analytical Procedures / Ian M. Bird -- CH. 19 Analysis of Cellular Phosphoinositides and Phosphoinositols by High-Performance Liquid Chromatography / Ian M. Bird -- CH. 20 Cytosolic Free Calcium Measurements in Single Cells Using Calcium-Sensitive Fluorochromes / Baljit S. Moonga / Michael Pazianas / Christopher L.H. Huang / Peter J.R. Bevis / Vijay Shankar / Christopher Bax / Mone Zaidi / A.S.M. Towhidul Alam -- CH. 21 Membrane Permeabilization with Bacterial Toxins / C. Lindsay Bashford -- CH. 22 Measurement of Ion Fluxes and pH Gradients Across Cell Membranes / C. Lindsay Bashford -- CH. 23 Ligand Binding and Processing: The Perfused Liver as a Model System / Livia A. Casciola-Rosen / Ann L. Hubbard / Carol A. Renfrew -- CH. 24 Binding of Protein-Ligands to Cell-Surface Receptors / David A.W. Grant -- Appendix: 1. Density Gradient Media -- Appendix: 2. Balanced Salt Solutions
Summary Expert practitioners combine the traditional basic techniques of membrane isolation and analysis with modern advances in molecular biology, immunology, and clinical sciences to devise analytical techniques that are successful for expert and novice alike. The techniques are used in the isolation and compositional analysis of membranes and address animal, plant, and microbial cells. The topics covered in this first volume include the isolation of subcellular fractions, centrifugation, the extraction and quantitation of lipids, the separation and analysis of proteins, and the isolation and characterization of glycosylated molecules, glycoproteins, proteoglycans, and glycolipids
Analysis celmembranen
cell membranes
isolatie
isolation
samenstelling
composition
biologische technieken
biological techniques
protocollen
protocols
Biochemistry
Membranes
Biochemie
Membranen
Bibliography Includes bibliographical references and indexes
Subject Cell membranes
Cell Membrane -- chemistry
Cell Membrane
Cell Membrane -- ultrastructure
Membrane Lipids -- analysis
Membrane Proteins -- analysis
Cell membranes
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Graham, J. M. (John M.), 1943- editor.
Higgins, Joan A., editor
ISBN 0896032361
9780896032361
9781592595068
1592595065
Other Titles Isolation and analysis