| Description |
1 online resource (xv, 268 pages) : illustrations (some color) |
| Series |
Methods in molecular biology ; 466 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 466
|
| Contents |
Isolation and propagation of glomerular mesangial cells / Paolo Mené and Antonella Stoppacciaro -- Isolation and primary culture of human proximal tubule cells / David A. Vesey [and others] -- Propagation and culture of renal fibroblasts / Lauren Grimwood and Rosemary Masterson -- Small animal models of kidney disease : a review / Tim D. Hewitson, Takahiko Ono, and Gavin J. Becker -- Measurement of glomerular filtration rate in conscious mice / Zhonghua Qi and Matthew D. Breyer -- Laser capture microdissection of kidney tissue / Robert P. Woroniecki and Erwin P. Bottinger -- Quantitative gene expression analysis in kidney tissues / Chris Tikellis [and others] -- In vivo imaging of leukocyte recruitment to glomeruli in mice using intravital microscopy / A. Richard Kitching, Michael P. Kuligowski, and Michael J. Hickey -- Using in situ hybridization to localize renal gene expression in tissue sections / Ian A. Darby, Alexis Desmoulière, and Tim D. Hewitson -- Immuno and lectin histochemistry for renal light microscopy / Tim D. Hewitson and Lauren Grimwood -- Immuno and lectin histochemistry for renal electron microscopy / Mitsuru Nakajima -- Pimonidazole adduct immunohistochemistry in the rat kidney : detection of tissue hypoxia / Christian Rosenberger [and others] -- Identification of apoptosis in kidney tissue sections / Glenda Gobe -- Cell-populated floating collagen lattices : an in vitro model of parenchymal contraction / Kristen J. Kelynack -- Mechanical stretch-induced signal transduction in cultured mesangial cells / Joan Krepinsky -- Determination of collagen content, concentration, and sub-types in kidney tissue / Chrishan S. Samuel -- SELDI-TOF mass spectrometry-based protein profiing of kidney tissue / Eleni Giannakis [and others] -- In vivo transfer of small interfering RNA or small hairpin RNA targeting glomeruli / Yoshitsugu Takabatake, Yoshitaka Isaka, and Enyu Imai |
| Summary |
As the expense of treating a growing number of end-stage kidney disease patients increases, greater attention has been paid to prevention and early treatment. The study of renal disease, however, suffers due to the complex nature of renal anatomy and physiology and the plethora of different cell types found in the kidney. In Kidney Research: Experimental Protocols, top experts in the field seek to aid researchers by providing a number of specialized techniques developed to examine this intricate system. Through both well-established and novel methods, this volume explores the preparation and culture of the main cell types used to study renal disease mechanisms, the common animal models used to mimic the various forms of human renal disease, and specific applications and techniques used in vivo and in vitro. Written in the highly successful Methods in Molecular Biology series format, the chapters contain step-by-step, readily reproducible laboratory protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Essential and cutting-edge, Kidney Research: Experimental Protocols delivers invaluable guidance to new and experienced laboratory researchers delving into the sophisticated study of the kidney |
| Analysis |
nierziekten |
|
kidney diseases |
|
nieren |
|
kidneys |
|
laboratoriummethoden |
|
laboratory methods |
|
protocollen |
|
protocols |
|
Human Pathology |
|
Humane pathologie |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
|
Print version record |
| Subject |
Kidneys -- Diseases.
|
|
Medical laboratory technology.
|
|
Clinical Laboratory Techniques
|
|
Kidney Diseases -- physiopathology
|
|
Kidney Diseases
|
|
Medical laboratory technology
|
|
Kidneys -- Diseases
|
|
Maladies du rein -- physiopathologie -- Cahiers de laboratoire.
|
|
Techniques et procédures de laboratoire -- Cahiers de laboratoire.
|
|
Rein -- Maladies.
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
|
Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Hewitson, Tim D.
|
|
Becker, Gavin J.
|
| ISBN |
9781597453523 |
|
1597453528 |
|
