Title Plant pathology : techniques and protocols / edited by Robert Burns

Published New York : Humana Press, ©2009

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Description 1 online resource (ix, 321 pages) : illustrations Series Methods in molecular biology, 1064-3745 ; 508 Springer protocols Methods in molecular biology (Clifton, N.J.) ; v. 508 Springer protocols (Series) Contents 1. Bioassay for diagnosis of plant viruses / Isla A. Browning -- 2. Development of enzyme linked, tissue blot and dot blot immunoassays for plant virus detection / Hei-ti Hsu -- 3. Immunisation strategies for antibody production / Eobert Burns -- 4. Preparation of immunogens and production of antibodies / Hei-ti Hsu [and others] -- 5. Enzyme-linked immunosorbent assay detection of ralstonia solanacearum in potatoes / Dirk U. Bellstedt -- 6. Production of monoclonal antibodies to plant pathogens / Christopher R. Thornton -- 7. Enzyme-linked immunosorbent assay for the detection and identification of plant pathogenic bacteria / Blanka Kokoskova and Jaap D. Janse -- 8. Indirect immunofluorescence microscopy for the detection and identification of plant pathogenic bacteria (in particular for Ralstonia solanacearum) / Jaap D. Janse and Blanka Kokoskova -- 9. Detection of four major bacterial potato pathogens / Ellen M. Kerr, Greig Cahill and Karen Fraser -- 10. Erwinia amylovora / Antonet M. Svircev [and others] -- 11. Use of fluorescent in situ hybridization in plant fungal identification and genotyping / Mohamed Hijri -- 12. Use of molecular methods for the detection of fungal spores / Elaine Ward -- 13. Identification of phytophthora fragariae var. rubi by PCR / Alexandra Schlenzig -- 14. Detection of double-stranded RNA elements in the plant pathogenic fungus Rhizoctonia solani / Nikki D. Charlton, Stellos M. Tavantzis, and Marc A. Cubeta 15. Immunocapture-PCR for plant virus detection / Vincent Mulholland -- 16. Multiplex polymerase chain reaction (PCR) and real-time multiplex PCR for the simultaneous detection of plant viruses / V. Pallas [and others] -- 17. Fluorescent-based techniques for viral detection, quantification, and characterization / Mathieu Rolland, Agnés Delaunay, and Emmanuel Jacquot -- 18. Analysis of population structures of viral isolates using single-strand conformation polymorphism method / Agnés Delaunay, Mathieu Rolland, and Emmanuel Jacquot -- 19. Direct detection of plant viruses in potato tubers using real-time PCR Neil Boonham [and others] -- 20. Accelerated soil bait assay for the detection of potato mop top virus in agricultural soil / Triona Davey -- 21. Detection of phytoplasmas of temperate fruit trees / Margit Laimer -- 22. PCR detection of potato cyst nematode / Alex Reid -- 23. Potato cultivar genome analysis / Alex Reid [and others] -- 24. Barley variety identification using SSRs / Cathy Southworth Summary Plant diseases can have an enormous impact on our lives. In a world where total crop failure can quickly lead to human misery and starvation, accurate diagnostics play a key role in keeping plants free from pathogens. In Plant Pathology: Techniques and Protocols, expert researchers provide methods which are vital to the diagnosis of plant diseases across the globe, addressing all three categories of plant pathology techniques: traditional, serological, and nucleic acid. Chapters examine recent and developing issues with crop identity and authenticity, allowing workers to genotype samples from two major food groups. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Authoritative and reader-friendly, Plant Pathology: Techniques and Protocols is an incredible guide which will soon prove to be indispensable, both to novices and expert researchers alike Analysis plantenziektekunde plant pathology plantenziekteverwekkers plant pathogens methodologie methodology diagnose diagnosis serologie serology protocollen protocols moleculaire plantenziektekunde molecular plant pathology diagnostiek diagnostics Diseases, Pests and Disorders of Plants (General) Plantenziekten, -plagen en -afwijkingen (algemeen) Bibliography Includes bibliographical references and index Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL Print version record digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL Subject Plant diseases. Plant Diseases Plant diseases Maladies des plantes -- Cahiers de laboratoire. Plantes -- Maladies. Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Burns, Robert, 1959- ISBN 9781597450621 1597450626

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