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Title Macrophages : methods and protocols / edited by Germain Rousselet
Published New York : Humana Press : Springer, [2018]
©2018

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Description 1 online resource (xii, 278 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; v. 1784
Methods in molecular biology (Clifton, N.J.) ; v. 1784.
Contents Culture of human monocyte-derived macrophages / Aoife Kelly, Aleksander M. Grabiec, and Mark A. Travis -- Simple multistep protocol for differentiating human induced pluripotent stem cells into functional macrophages / Chandrayana Mukherjee, Christine Hale, and Subhankar Mukhopadhyay -- Growing murine bone marrow-derived macrophages / Anaïs Assouvie, Lisa P. Daley-Bauer, and Germain Rousselet -- Immortalization of murine bone marrow-derived macrophages / Dominic De Nardo, Dhan V. Kalvakolanu, and Eicke Latz -- Isolation and identification of murine serous cavity macrophages / Calum C. Bain and Stephen J. Jenkins -- Isolation and identification of interstitial macrophages from the lungs using different digestion enzymes and staining strategies / Shaikh M. Atif, Sophie L. Gibbings, and Claudia V. Jakubzick -- Isolation and phenotyping of adult mouse microglial cells / Kathleen Grabert and Barry W. McColl -- Isolation and phenotyping of bone marrow macrophages / Marion Chalot -- Isolation and phenotyping of intestinal macrophages / Vanessa Petit -- Phenotyping tumor-associated macrophages / R. Ian Cumming and Yen-Rei A. Yu -- Activating murine macrophages In Vitro / Elizabeth Dalby -- Polarizing macrophages in vitro / Xuan Huang, Yong Li, Mingui Fu, and Hong-Bo Xin -- Viral replication assay in bone marrow-derived macrophages / Linda Roback and Lisa P. Daley-Bauer -- Macrophage bactericidal assays / Mourad Aribi -- Quantitative phagocytosis assays in primary and cultured macrophages / Fernando Montaño, Sergio Grinstein, and Roni Levin -- Observing frustrated phagocytosis and phagosome formation and closure using total internal reflection fluorescence microscopy (TIRFM) / Anna Mularski, Florence Marie-Anaïs, Julie Mazzolini, and Florence Niedergang -- Chromatin immunoprecipitation in macrophages / Germain Rousselet -- Transfecting macrophages / Andrea-Anneliese Keller, Marten B. Maess, Michael Schnoor, Berith Scheiding, and Stefan Lorkowski -- In vitro migration assays / Lewis Taylor, Carlota Recio, David R. Greaves, and Asif J. Iqbal -- Cytokine-induced acute inflammatory monoarticular arthritis / Adrian Achuthan, Ming-Chin Lee, Reem Saleh, Andrew J. Fleetwood, John A. Hamilton, and Andrew D. Cook -- Characterizing activation, proliferation, and ontogeny of murine macrophages in parasitic helminth infections / Dominik Rückerl -- Genetic models of macrophage depletion / Li Hua, Jiayuan Shi, Leonard D. Shultz, and Guangwen Ren -- Depleting macrophages in vivo with clodronate-liposomes / Stéphanie G. Moreno -- Cre driver mice targeting macrophages / Jiayuan Shi, Li Hua, Danielle Harmer, Peishan Li, and Guangwen Ren
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocol, viewed May 16, 2018)
Subject Macrophages -- Laboratory manuals
Macrophages.
Macrophages
Cellular biology (cytology)
Science -- Life Sciences -- Biology -- Molecular Biology.
Macrophages
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Rousselet, Germain, editor
ISBN 9781493978373
1493978373
1493978365
9781493978366