| Description |
1 online resource |
| Series |
New developments in mass spectrometry ; no. 10 |
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New developments in mass spectrometry ; 10.
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| Contents |
Intro -- Title -- Copyright -- Contents -- Chapter 1 State-of-the-art in LC-MS Approaches for Probing the Polar Metabolome -- 1.1 Introduction: Metabolomics and the Polar Metabolome -- 1.2 LC Technologies: General Introduction -- 1.3 LC Technologies to Probe the Polar Metabolome -- 1.3.1 Hydrophilic Interaction Liquid Chromatography (HILIC) -- 1.3.2 Reversed-phase Liquid Chromatography (RPLC) with Derivatization -- 1.3.3 Ion-pair Chromatography (IPC) -- 1.3.4 Ion Exchange Chromatography (IEC) -- 1.3.5 Mixed-mode Chromatography (MMC) and Multimode Chromatography |
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1.3.6 Two-dimensional Liquid Chromatography (2D-LC) -- 1.4 Conclusions -- Abbreviations -- References -- Chapter 2 Chemical Derivatization for Polar Metabolome Analysis -- 2.1 Introduction -- 2.2 Chemical Derivatization LC-MS Analysis of Targeted Polar Metabolites -- 2.2.1 Amino Acid and Derivative Analysis -- 2.2.2 Nucleotide Analysis -- 2.2.3 Sugar Analysis -- 2.2.4 Small Organic Acid Analysis -- 2.3 Untargeted Analysis of Polar and Less-polar Metabolites -- 2.3.1 Main Features of CIL LC-MS -- 2.3.2 Data Processing in CIL LC-MS -- 2.3.3 Applications of CIL LC-MS -- 2.4 Concluding Remarks |
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Acknowledgements -- References -- Chapter 3 Ion Pair Liquid Chromatography-Mass Spectrometry for Probing the Polar Metabolome -- 3.1 Introduction -- 3.2 Ion Pair Liquid Chromatography (IP-LC) -- 3.2.1 Retention Mechanism of IP-LC -- 3.2.2 Critical Parameters of IP-LC -- 3.3 Mass Spectrometry -- 3.3.1 Targeted Analysis -- 3.3.2 Untargeted Analysis -- 3.4 Sample Preparation -- 3.4.1 Quenching -- 3.4.2 Extraction -- 3.4.3 Post-extraction -- 3.5 Ion Pair Liquid Chromatography-Mass Spectrometry-based Metabolomics -- 3.5.1 Central Carbon Metabolism -- 3.5.2 Amino Acids -- 3.5.3 Nucleotides |
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3.6 Potential Pitfalls in IP-LC-MS-based Metabolomics -- 3.6.1 Separation of Isomers -- 3.6.2 Ion Suppression -- 3.6.3 Detrimental Effect of Metal Cations -- 3.6.4 System Contamination -- References -- Chapter 4 Hydrophilic Interaction Chromatography-Mass Spectrometry (HILIC-MS) Approaches for Probing the Polar Metabolome -- 4.1 HILIC-MS: A Powerful Analytical Approach for the Qualitative and Quantitative Analysis of the Polar Cellular Metabolome -- 4.2 From Stationary Phases to Analytical Conditions and Retention Mechanisms -- 4.2.1 Stationary Phases According to Chemical Structure |
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4.2.2 Gradient Elution, Buffers and Ionic Strength of Mobile Phases -- 4.2.3 What is Known About Retention Mechanisms in HILIC -- 4.3 Selectivity, Sensitivity and Reproducibility of HILIC-MS approaches -- 4.4 Applications: From Single-pathway Targeted Quantification to Multiple Pathway Targeted and Untargeted Profiling -- 4.5 Isotopic Profiling -- 4.6 Conclusive Remarks -- Acknowledgements -- References -- Chapter 5 Advances in Solid Phase Microextraction (SPME) for Metabolomics -- 5.1 Introduction to Solid Phase Microextraction -- 5.2 Application of SPME in Foodomics |
| Summary |
Discussing the state-of-the-art of the proposed topics in one single book for probing the polar metabolome, using relevant examples, is unique and needed in the metabolomics field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from digital title page (viewed on July 29, 2021) |
| Subject |
Metabolites -- Analysis
|
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Mass spectrometry.
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Metabolome
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Mass Spectrometry
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mass spectrometry.
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Analytic.
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Biochemistry.
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Life Sciences.
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SCIENCE.
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Chemistry.
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Mass spectrometry
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| Form |
Electronic book
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| Author |
Ramautar, Rawi, editor
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| ISBN |
9781839163524 |
|
1839163526 |
|
9781839163531 |
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1839163534 |
|
