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Title Lymphoma : methods and protocols / edited by Tim Illidge, Peter W.M. Johnson
Published Totowa, N.J. : Humana Press, ©2005


Description 1 online resource (xvi, 342 pages) : illustrations (some color)
Series Methods in molecular medicine, 1543-1894 ; 115
Methods in molecular medicine ; 115. 1543-1894
Contents Purification of primary malignant B-cells and immunoblot analysis of Bcl-2 family proteins / Claire Dallman and Graham Packham -- Outcome prediction by gene expression profiling in diffuse large B-cell lymphoma / Kim Last, Silvana Debarnardi, and T. Andrew Lister -- Demonstration of a germinal center immunophenotype in lymphomas by immunochemistry and flow cytometry / Andrew Jack [and others] -- Karyotyping lymph node biopsies in non-Hodgkin's lymphoma / Fiona M. Ross and Christine J. Harrison -- Identification of Lymphoma-associated antigens using SEREX / Amanda P. Liggins, Barbara A. Guinn, and Alison H. Banham -- Determining mutational status of immunoglobulin V genes in chronic lymphocytic leukemia : a useful prognostic indicator / Surinder S. Sahota [and others] -- Idiotype gene rescue in follicular lymphoma / Katy McCann [and others] -- Immunoglobulin gene mutation patterns and heterogeneity of MZL / Mariella Dono and Manlio Ferrarini -- T-cell receptor molecular diagnosis of T-cell lymphoma / Elizabeth Hodges, Anthony P. Williams, and John L. Smith
Cloning of immunoglobulin chromosomal translocations by long-distance inverse polymerase chain reaction / E. Loraine Karran, Takashi Sonoki, and Martin J.S. Dyer -- Sequential fluorescence in situ hybridization analysis for trisomy 12 in B-cell chronic lymphocytic leukemia / Viktoria Hjalmar -- Splenic marginal zone lymphoma : 7q abnormalities / Rachel E. Ibbotson, Anton E. Parker, and David G. Oscier -- BCL-6: rearrangement and mutation in lymphoma / Simon D. Wagner and Jaspal S. Kaeda -- Antibody techniques used in the study of ALK-positive lymphoma / Karen Pulford, H.M. Robertson, and M. Jones -- Identification of anaplastic lymphoma kinase variant translocations using 5'RACE / Luis Hernandez and Elias Campo -- Follicular Lymphoma : quantitation of minimal residual disease by PCR of the t(14;18) translocation / John G. Gribben and Angela Darby
Summary The tremendous progress made over the last few years in our molecular understanding of lymphocyte biology and lymphoma classification promises to have a major impact on clinical practice. In Lymphoma: Methods and Protocols, leading clinical investigators from world renowned laboratories describe in step-by-step detail the latest molecular techniques being used to better understand, classify, and treat lymphoma. Among the highlights are methods to use immunoglobulin gene rearrangements as markers of clonality, to exploit patterns of somatic mutation in the variable regions to indicate at which stage transformation occurred, and to apply gene arrays to the question of biological heterogeneity in morphologically similar diseases. Research methodologies that are highly likely to become routine practice in the future, such as DNA microarray and immunoglobulin V-gene rearrangements, and measurement of minimal disease, are included. There are also molecular techniques for producing novel therapeutics, such as a DNA vaccine with patient-specific sequences derived from the lymphoma in question. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Lymphoma: Methods and Protocols offers oncologists, hematologists, and clinical scientists a diverse collection of readily reproducible techniques for the molecular diagnosis and prognostic evaluation of lymphomas
Bibliography Includes bibliographical references and index
Notes English
Subject Lymphomas -- Molecular aspects -- Laboratory manuals
Medical laboratory technology.
Genetics -- Technique.
Clinical Laboratory Techniques
Genetic Techniques
MEDICAL -- Oncology.
HEALTH & FITNESS -- Diseases -- Cancer.
Medical laboratory technology
Genetics -- Technique
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Illidge, Timothy.
Johnson, P. W. M.
LC no. 2004026632
ISBN 1588291596