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Title Lymphoma : methods and protocols / edited by Ralf Küppers
Edition Second edition
Published New York, NY : Humana Press : Springer, [2019]


Description 1 online resource (xii, 439 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 1956
Methods in molecular biology (Clifton, N.J.) ; v. 1956. 1064-3745
Contents Origin and pathogenesis of B cell lymphomas / Marc Seifert, René Scholtysik, and Ralf Ku¨ppers -- Flow cytometry for non-hodgkin and hodgkin lymphomas / Emily Glynn, Lori Soma, David Wu, Brent L. Wood, and Jonathan R. Fromm -- Laser-based microdissection of single cells from tissue sections and PCR analysis of rearranged immunoglobulin genes from isolated normal and malignant human B cells / Ralf Ku¨ppers, Markus Schneider, and Martin-Leo Hansmann -- PCR genescan and heteroduplex analysis of rearranged immunoglobulin or T-cell receptor genes for clonality diagnostics in suspect lymphoproliferations / Elke Boone, Kim C. Heezen, Patricia J. T. A. Groenen, and Anton W. Langerak -- Expression cloning of antibodies from single human B cells / Hedda Wardemann and Christian E. Busse -- Studying the replication history of human B lymphocytes by real-time quantitative (RQ-)PCR. / Menno C. van Zelm -- Stereotyped B cell receptor immunoglobulins in B cell lymphomas / Andreas Agathangelidis, Fotis Psomopoulos, and Kostas Stamatopoulos -- Flow cytometric MRD detection in selected mature B-cell malignancies / Sebastian Bo¨ttcher -- MRD detection in B-cell non-hodgkin lymphomas using Ig gene rearrangements and chromosomal translocations as targets for real-time quantitative PCR / Christiane Pott, Monika Bru¨ggemann, Matthias Ritgen, Vincent H. J. van der Velden, Jacques J. M. van Dongen, and Michael Kneba -- Generation of whole genome bisulfite sequencing libraries from very low DNA input / Dieter Weichenhan, Charles D. Imbusch, Qi Wang, Benedikt Brors, and Christoph Plass -- FISH and FICTION in lymphoma research / Maciej Giefing and Reiner Siebert -- NGS-based high-throughput screen to identify microRNAs regulating growth of B-cell lymphoma / Joost Kluiver, Fubiao Niu, Ye Yuan, Klaas Kok, Anke van den Berg, and Agnieszka Dzikiewicz-Krawczyk -- RNA sequencing in B-cell lymphomas / Da Wei Huang, Moez Dawood, Calvin A. Johnson, and Roland Schmitz -- Studying cancer heterogeneity by single-cell RNA sequencing / Johannes W. Bagnoli, Lucas E. Wange, Aleksandar Janjic, and Wolfgang Enard -- Evaluation of whole genome sequencing data / Daniel Hu¨bschmann and Matthias Schlesner -- Protocols for CRISPR-Cas9 screening in lymphoma cell lines / Daniel E. Webster, Sandrine Roulland, and James D. Phelan -- Lymphoma and leukemia cell vulnerabilities and resistance identified by compound library screens / Katarzyna Tomska, Sebastian Scheinost, and Thorsten Zenz -- Proximity ligation assay / Ryan M. Young -- Phosphoproteomic analysis of signaling pathways in lymphomas / Bjo¨rn H€aupl, Henning Urlaub, and Thomas Oellerich -- Ultrasensitive detection of circulating tumor DNA in lymphoma via targeted hybridization capture and deep sequencing of barcoded libraries / Miguel Alcaide, Christopher Rushton, and Ryan D. Morin
Summary This second edition volume expands on the previous edition with descriptions of recent developments in the field. The new and updated chapters discuss the latest approaches for studying single cell RNA sequencing, whole genome sequencing data, CRISPR/Cas9-mediated gene knockout, protein interactions by a proximity ligation assay, and detection of circulating cell-free tumor DNA in lymphomas. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed February 19, 2019)
Subject Lymphomas -- Laboratory manuals
Lymphoma, B-Cell
Medical -- Oncology.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Küppers, Ralf, editor
ISBN 9781493991518