Machine generated contents note: Engineering fluorescent proteins / A. Miyawaki / T. Nagai / H. Mizuno -- Higher harmonic generation microscopy / C.-K. Sun -- Live cell spinning disk microscopy / R. Graf / J. Rietdorf / T. Zimmermann -- Total internal reflection fluorescence microscopy in single molecule nanobioscience / T. Wazawa / M. Ueda -- Fluorescence correlation spectroscopy with autofluorescent proteins / T. Kohl / P. Schwille -- Fluorescence lifetime imaging microscopy (FLIM) / E.B. van Munster / T.W.J. Gadella -- Fluorescence recovery after photoleaching : application to nuclear proteins / A.B. Houtsmuller -- Deconvolution microscopy / J.-B. Sibarita -- Spectral imaging and linear unmixing in light microscopy / T. Zimmermann