| Description |
1 online resource (162 pages) |
| Contents |
Cover; Half Title; Title Page; Copyright Page; The Author; Table of Contents; Chapter 1: Introduction; References; Chapter 2: Energy Levels in Molecules and Transition Between Them; I. Introduction; II. Energy Levels; III. Internal Conversion and Vibrational Relaxation; IV. Fluorescence; V. Intersystem Crossing; VI. Phosphorescence; VII. Frank-Condon Principle; VIII. Luminescence Lifetime; IX. Fluorescence Quantum Yield; X. Stern-Volmer Equation; XI. Excitation Energy Transfer Between Singlet States; XII. Effects of Intermolecular Interactions on Electron-Vibrational Transitions in Molecules |
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XIII. Scheme of Steady-State SpectrofluorimeterReferences; Chapter 3: Spectroscopic Properties of Isolated Protein Chromophores; I. Introduction; II. Tryptophan; A. Absorption Spectrum; B. Emission Spectra; C. Emission Lifetime; D. Analytical Description of the Fluorescence Spectrum Shape; E. Fluorescence Quantum Yield; F. Acid-Base Properties in the Ground and Excited States; G. Fluorescence Quenching by Protein Groups; 1. Carboxylate (â#x80;#x93;COOâ#x88;#x92;) and Carboxyl (â#x80;#x93;COOH) Groups; 2. Amide (â#x88;#x92;CONH2) and Peptide (â#x80;#x93;COâ#x80;#x93;NHâ#x80;#x93;) Groups; 3. Ammonium (â#x80;#x93;NH3) and Amino (â#x80;#x93;NH2) Groups |
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4. Guanidine Group5. Imidazole and Imidazolium of Histidine; 6. Sulfur-Containing Groups (â#x80;#x93;SH, â#x80;#x93;Sâ#x88;#x92;, â#x80;#x93;Sâ#x80;#x93;Sâ#x80;#x93;, â#x80;#x93;Câ#x80;#x93;Sâ#x80;#x93;Câ#x80;#x93;); H. Temperature Dependence of Bimolecular Quenching of Indole Fluorescence; III. Tyrosine; A. Absorption Spectrum; B. Emission Spectrum; C. Emission Lifetime; D. Fluorescence Quenching by Protein Groups; 1. Carboxylate (â#x80;#x93;COOâ#x88;#x92;) and Carboxyl (â#x80;#x93;COOH) Groups; 2. Amide (â#x80;#x93;CONH2) and Peptide (â#x80;#x93;COâ#x80;#x93;NHâ#x80;#x93;) Groups; 3. Ammonium (â#x80;#x93;NH3+) and Amino (â#x80;#x93;NH2) Group; 4. Guanidine Group; 5. Imidazole and Imidazolium of Histidine; 6. Sulfur-Containing Groups |
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IV. PhenylalanineA. Absorption Spectrum; B. Emission Spectrum; C. Fluorescence Quantum Yield; References; Chapter 4: Protein Luminescence; I. Introduction; II. Position and Shape of Protein Fluorescence Spectra; A. Tryptophan Fluorescence; B. Tyrosine Fluorescence; C. Phenylalanine Fluorescence; III. Fluorescence Quantum Yields of Proteins; A. Tryptophan Fluorescence; B. Tyrosine Fluorescence; C. Phenylalanine Fluorescence; IV. Fluorescence Decay of Proteins; A. Tryptophan Fluorescence; B. Tyrosine Fluorescence; C. Phenylalanine Fluorescence |
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V. General Strategy of Luminescent Study of ProteinsVI. Fluorescence Parameters as a Measure of the Fraction of Conversion from One State to Another in Proteins; VII. Search for Intermediate States; VIII. Studies of Temperature Dependencies of Protein Fluorescence Parameters; IX. Studies of pH Dependencies of Protein Fluorescence; X. Studies of Effects of Ionic Strength on Protein Fluorescence; XI. Studies of Effects of Denaturants on Protein Fluorescence; XII. Studies of Interactions of Proteins with Low-Molecular-Mass Compounds by Fluorescence Method |
| Summary |
"Luminescent Spectroscopy of Proteins is devoted to the method of intrinsic protein luminescence, one of the most popular experimental methods in modern biophysics and biochemistry. The book discusses general physical principles of the luminescence method; spectral properties of the main protein chromophores; and protein luminescence and its use for studies on structural, physico-chemical, and functional properties of proteins. Principles of luminescent spectroscopy are illustrated by real-life applications and problems. Luminescent Spectroscopy of Proteins will be an excellent reference for biophysicists, biochemists, analytical chemists, and other scientists interested in this topic."--Provided by publisher |
| Notes |
XIII. Method of Selective Fluorescence Quenching |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Print version record |
| Subject |
Proteins -- Analysis.
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Proteins -- Spectra.
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Luminescence spectroscopy.
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Luminescent Proteins -- analysis
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Spectrum Analysis
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Luminescent Measurements
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Luminescence spectroscopy
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Proteins -- Analysis
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Proteins -- Spectra
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Spectroscopie de luminescence.
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Protéines -- Analyse.
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| Form |
Electronic book
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| ISBN |
9781351091114 |
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1351091115 |
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9781351082686 |
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135108268X |
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9781351099561 |
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1351099566 |
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