Title Green fluorescent protein : applications and protocols / edited by Barry W. Hicks

Published Totowa, NJ : Humana Press, 2002

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Description 1 online resource (xiv, 393 pages) : illustrations Series Methods in molecular biology ; v. 183 Methods in molecular biology (Clifton, N.J.) ; v. 183 Contents Amplification of representative cdna samples from microscopic amounts of invertebrate tissue to search for new genes / M.V. Matz -- Use of coba and cysga as red fluorescent indicators / C.A. Roessner -- Circular permutation of the green fluorescent protein / S. Topell and R. Glockshuber -- Evolutionary design of generic green fluorescent protein biosensors / N. Doi and H. Yanagawa -- Random insertion of green fluorescent protein into the regulatory subunit of cyclic adenosine monophosphate-dependent protein kinase / P.J. Baehler [and others] -- Circular mrna encoding for monomeric and polymeric green fluorescent protein / R. Perriman -- Fluorescence lifetime imaging (flim) of green fluorescent fusion proteins in living cells / A. Periasamy [and others] -- Fluorescence resonance energy transfer (fret) applications using green fluorescent protein. Energy transfer to the endogenous chromophores of phycobilisome light-harvesting complexes / J.J. van Thor and K.J. Hellingwerf -- Bioluminescence resonance energy transfer assays for protein-protein interactions in living cells / Y. Xu, C.H. Johnson and D. Piston -- Whole-body fluorescence imaging with green fluorescence protein / R.M. Hoffman -- Drug-induced translocation of nucleolar proteins fused to green fluorescent protein / B.C. Valdez and L. Perlaky -- Light-induced nuclear targeting of phytochromeb-sgreen fluorescent protein in plants / A. Nagatani and T. Matsushita -- Mechanisms of protein trafficking. Two different signal sequences fused to green fluorescent protein to study mitochondrial import / H. Weiner -- Analysis of nucleocytoplasmic transport using green fluorescent protein / R.H. Stauber -- Transgenic bovine embryo selection using green fluorescent protein / A.W. Chan, K.Y. Chong and G. Schatten -- Development of glycosylphosphatidylinositol-anchored enhanced green fluorescent protein. One-step visualization of gpi fate in global tissues and ubiquitous cell surface marking / G. Kondoh -- Transgenic zebrafish expressing green fluorescent protein / E. Shafizadeh, H. Huang and S. Lin -- Transgenic insects. Expressing green fluorescent protein-silk fibroin light chain fusion protein in transgenic silkworms / H. Mori -- Green fluorescent protein in transgenic plants. Brassica transformation / C.N. Stewart, Jr., M.D. Halfhill and R.J. Millwood -- Green fluorescent protein calcium biosensors. Calcium imaging with GFP cameleons / A. Varadi and G.A. Rutter -- Green fluorescent protein fluobody immunosensors. Immunofluorescence with GFP -antibody fusion proteins / A. Schots and J.M. van der Wolf -- Green fluorescent protein-based protein kinase biosensor substrates / S. Ulrich and K. Shokat -- Green fluorescent protein urea sensors. Uropathogenic proteus mirabilis / C. Coker, H. Zhao and H.L. Mobley -- Using green fluorescent protein to monitor measles virus cell-to-cell spread by time-lapse confocal microscopy / W.P. Duprex and B.K. Rima -- Tracking and selection of retrovirally transduced murine bone marrow cells using green fluorescent protein / J. Bagley and J. Iacomini -- Green fluorescent protein as a reporter of adenovirus-mediated gene transfer and expression in the hypothalamic-neurohypophyseal system / E.C. Vasquez and A.K. Johnson -- Enhancement of green fluorescent protein expression in adeno-associated virus with the woodchuck hepatitis virus post-transcriptional regulatory element / J.E. Loeb, M.D. Weitzman and T.J. Hope -- Construction of infectious simian varicella virus expressing green fluorescent protein / R. Mahalingam and D.H. Gilden -- Green fluorescent protein in retroviral vector constructs as marker and reporter of gene expression for cell and gene therapy applications / N. Eliopoulos and J. Galipeau Summary Green fluorescent protein (GFP), a critically important marker protein, allows direct visualization in live samples without the aid of additional sample preparation. In Green Fluorescent Protein: Applications and Protocols, Barry W. Hicks and a team of well-practiced experimentalists demonstrate the power and the versatility of this marker with a cutting-edge collection of readily reproducible GFP techniques. These include methods for detecting and imaging GFP (FRET, BRET, and whole-body imaging), for using GFP to create and select for transgenic organisms, for its use as a biosensor, and for studying viruses. Each method is described in step-by-step detail to ensure robust results and can be used in applications ranging from agriculture and botany to human gene therapy. Extensive notes review the materials required, explain the scientific basis of each step and possible sources of error, and provide tips for overcoming problems. Highly practical and state-of-the-art, Green Fluorescent Protein: Applications and Protocols makes available to all biological and biomedical experimentalists a full array of the powerful new GFP techniques Bibliography Includes bibliographical references and index Notes Print version record Subject Green fluorescent protein -- Laboratory manuals Green fluorescent protein. Luminescent Proteins -- analysis Indicators and Reagents -- analysis SCIENCE -- Life Sciences -- Molecular Biology. Green fluorescent protein Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Hicks, Barry W. ISBN 9781592592807 0896039056 9780896039056 1592592805 1280830654 9781280830655

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