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Title Bacterial pangenomics : methods and protocols / edited by Alessio Mengoni, Marco Galardini, Marco Fondi
Published New York, NY : Humana Press, [2015]
©2015

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Description 1 online resource (x, 298 pages) : illustrations (some color)
Series Methods in Molecular Biology, 1940-6029 ; 1231
Methods in molecular biology (Clifton, N.J.) ; v. 1231. 1064-3745
Contents Pulsed field gel electrophoresis and genome size estimates/ Rosa Alduina and Annalisa Pisciotta -- Comparative analyses of extrachromosomal bacterial replicons, identification of chromids, and experimental evaluation of their indispensability / Lukasz Dziewit and Dariusz Bartosik -- Choice of next-generation sequencing pipelines / F. Del Chierico [and others] -- Pyrosequencing protocol for bacterial genomes / Ermanno Rizzi -- Bacterial metabarcoding by 16S rRNA gene ion torrent amplicon sequencing / Elio Fantini [and others] -- Illumina-solexa sequencing protocol for bacterial genomes / Zhenfei Hu, Lei Cheng, and Hai Wang -- High-throughput phenomics / Carlo Viti [and others] -- Comparative analysis of gene expression : uncovering expression conservation and divergence between Salmonella enterica serovar typhimurium strains LT2 and 14028S / Paolo Sonego [and others] -- Raw sequence data and quality control / Giovanni Bacci -- Methods for assembling reads and producing contigs / Valerio Orlandini, Marco Fondi, and Renato Fani -- Mapping contigs using CONTIGuator / Marco Galardini, Alessio Mengoni, and Marco Bazzicalupo -- Gene calling and bacterial genome annotation with BG7 / Raquel Tobes [and others] -- Defining orthologs and pangenome size metrics / Emanuele Bosi, Renato Fani, and Marco Fondi -- Robust identification of orthologues and paralogues for microbial pan-genomics using GET_HOMOLOGUES : a case study of pIncA/C plasmids / Pablo Vinuesa and Bruno Contreras-Moreira -- Genome-scale metabolic network reconstruction / Marco Fondi and Pietro Liò -- From pangenome to panphenome and back / Marco Galardini, Alessio Mengoni, and Stefano Mocali -- Genome-wide detection of selection and other evolutionary forces / Zhuofei Xu and Rui Zhou -- Integrated microbial genome resource of analysis / Alice Checcucci and Alessio Mengoni
Summary Bacterial genomics is a mature research interdisciplinary field, which is approached by ecologists, geneticists, bacteriologists, molecular biologists and evolutionary biologists working in medical, industrial and basic science. Thanks to the large diffusion of bacterial genome analysis, Bacterial Pangenomics: Methods and Protocols is able to provide the most recent methodologies about the study of bacterial pangenomes by covering the three major areas: the experimental methods for approaching bacterial pangenomics, the bio informatic pipelines for analysis and annotation of sequence data and finally the methods for inferring functional and evolutionary features from the pangenome. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Bacterial Pangenomics: Methods and Protocols will serve as a field guide for both qualified bacterial genomics investigators who want to update their technical knowledge, for less experienced researchers who want to start working with bacterial genomics and pangenomics, as well as serving as a manual and supplemental textbook for graduate students of genomics and bioinformatics
Analysis biomedische wetenschappen
biomedicine
bacteriologie
bacteriology
genetische modificatie
genetic engineering
microbiologie
microbiology
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed November 14, 2014)
Subject Bacterial genetics.
Bacterial genomes.
Bacteriology.
Genome, Bacterial -- genetics
Genome, Bacterial
Bacteriology
Bacteriological Techniques -- methods
Computational Biology -- methods
Genetic Techniques -- methods
Bacteriology (non-medical)
Genetic engineering.
Microbiology (non-medical)
Science -- Life Sciences -- Bacteriology.
Science -- Life Sciences -- Biology -- Microbiology.
Science -- Life Sciences -- Genetics & Genomics.
Bacterial genetics
Bacterial genomes
Bacteriology
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Mengoni, Alessio, editor
Galardini, Marco, editor
Fondi, Marco, editor
ISBN 9781493917204
149391720X
1493917196
9781493917198