| Description |
1 online resource (ix, 207 pages) : illustrations |
| Contents |
Introduction -- Part 1: A Short History of the lac System from its Beginning to 1978 -- 1.1 From Noah to Pasteur: Adaptation in Yeast -- 1.2 Adaptation in Bacillus subtilis and Escherichia coli -- 1.3 Mutants in the lac System of Escherichia coli -- 1.4 Inducers of the lac System and the Discovery of Permease -- 1.5 Negative Control through Repressor -- 1.6 Further Evidence for Negative Control through Repressor -- 1.7 The Triumph of the Jacob-Monod Theory -- 1.8 Minor Defects in the Jacob-Monod Theory -- 1.9 Isolation of the Lac and Î" CI Repressors |
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1.10 Putting the lac Genes on Phage Ï?80 or Î"1.11 Lac and Î" CI Repressors Bind to their Operator DNAs -- 1.12 Making Grams of Lac Repressor to Determine its Sequence -- 1.13 Mutations in lacI Suggest a Modular Structure of Repressor -- 1.14 Millerâ€?s Analysis of the I Gene: Climbing Mount Everest -- 1.15 Positive Control through the CAP Protein -- 1.16 Isolation and Sequence of lac Operator -- 1.17 Chemical DNA Sequencing -- 1.18 Steric Hindrance is the Mechanism of Repression -- 1.19 Chemical Synthesis of lac Operator DNA |
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1.20 Lac Repressor-Operator Complex Binds to Nitrocellulose1.21 Polyacrylamide Gel Electrophoresis of Protein-DNA Complexes -- 1.22 Fusion of and Complementation within the lacZ Gene -- 1.23 Isolation of Lac Permease -- 1.24 The End of an Era -- Part 2: Misinterpretation -- 2.1 The Interpretation of Experiments is Sometimes Wrong -- 2.2 Adaptation Explained by Self-Replicating Genes -- 2.3 The Kinetics of Adaptation -- 2.4 Lac Repressor is RNA -- 2.5 Operator is RNA or Protein -- 2.6 Isolation of Lac Repressor -- 2.7 Isolation of Lac Permease and Arg Repressor |
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2.8 Genetic Proof that lac Operator is Dyadic2.9 The Core of Lac Repressor Binds to lac Operator -- 2.10 Lac Repressor Uses ß-Sheet to Recognize lac Operator -- 2.11 A Ï€-Helix Binds to a Hairpin Loop of lac Operator -- 2.12 Prediction of the Sequence of lac Operator -- 2.13 The Kinetics of lac Repressor-Operator Interaction -- 2.14 Lac Repressor Bound to O2 Acts as an Efficient Road Block -- 2.15 Auxiliary Operators Can be Disregarded in Repression -- 2.16 CAP Activates Transcription like Î" CI Repressor -- 2.17 In the Pitfall of Symmetry |
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2.18 The CAP-DNA Complex: Two Propositions2.19 The Adenine of Cyclic AMP Binds to DNA -- 2.20 The Structure of ß-Galactosidase -- 2.21 From Lamarck to Cairns -- 2.22 DNA Sequence Analysis: Not in this Century! -- 2.23 Protein Splicing -- 2.24 OI-Repressor-O2 Loops -- Part 3: The lac Operon, a Paradigm of Beauty and Efficiency -- 3.1 The New Perspective -- 3.2 Some Numbers and Concepts -- 3.3 Activation of the lac Promoter by CAP Protein -- 3.4 Repression of the lac Promoter by Lac Repressor -- 3.5 ß-Galactosidase, Lac Permease and Transacetylase |
| Summary |
"This book describes the history and present knowledge of a paradigmatic system, the lac operon of E. coli. The first part of the book presents the history of the operon and various schools of thought regarding genetic control in general. The second part presents a number of false interpretations and misconceptions and demonstrates how easily a scientist may deceive himself. The third and last part thoroughly covers the current state of knowledge of the lac operon including the importance of the auxiliary operators and discussions of several X-ray structures, one of which was published shortly before this book went into press. A unique combination of personal anecdotes and present-day science makes this book appealing to students, postdocs, active and retired researchers alike."--Jacket |
| Bibliography |
Includes bibliographical references and indexes |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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English |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| Subject |
Lac operon -- Research -- History
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Lac Operon
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NATURE -- Plants -- Mushrooms.
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Escherichia coli -- Genetics
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Forschung
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Lactose-Operon
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Genetica.
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| Genre/Form |
Electronic books
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History
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| Form |
Electronic book
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| ISBN |
9783110879476 |
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3110879476 |
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