| Description |
1 online resource (xi, 258 pages) : illustrations |
| Series |
Methods in molecular biology ; v. 164 |
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Methods in molecular biology (Clifton, N.J.) ; v. 164
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| Contents |
Purification of kinesin from the brain / S.A. Kuznetsov and V.I. Gelfand -- Rt-pcr for the identification of developmentally regulated novel members of the kinesin-like superfamily / N. Santama -- Expression cloning with pan kinesin antibodies / L.M. Ginkel and L. Wordeman -- Expression of kinesin in escherichia coli / M.F. Stock and D.D. Hackney -- Plasmids for expression of chimeric and truncated kinesin proteins / K.W. Waligora and S.A. Endow -- Preparation of recombinant kinesin superfamily proteins using the baculovirus system / N. Hirokawa and Y. Noda -- Assays for kinesin microtubule-stimulated atpase activity / D.D. Hackney and W. Jiang -- An improved microscope for bead and surface-based motility assays / N. Carter and R. Cross -- Use of photonic force microscopy to study single-motor-molecule mechanics / S. Jeney, E.L. Florin and J.K. Horber -- Assays for microtubule-destabilizing kinesins / A. Desai and C.E. Walczak -- Green fluorescent protein as a tag for molecular motor proteins / S.A. Endow -- In vitro reconstitution of endosome motility along microtubules / E. Nielsen [and others] -- Approaches to study interactions between kinesin motors and membranes / G. Morfini [and others] -- Microinjection methods for analyzing the functions of kinesins in early embryos / R.L. Morris [and others] -- The use of dominant negative mutants to study the function of mitotic motors in the in vitro spindle assembly assay in xenopus egg extracts / H. Boleti, E. Karsenti and I. Vernos -- A dominant negative approach for functional studies of the kinesin ii complex / V.I. Gelfand [and others] -- Identification of kinesin-associated proteins / L.C. Lindesmith, J. Kumar and M.P. Sheetz -- Assaying spatial organization of microtubules by kinesin motors / F. Nedelec and T. Surrey -- Crystallization of kinesin / M. Thormahlen, J. Muller and E. Mandelkow -- Structural analysis of the microtubule-kinesin complex by cryo-electron microscopy / F. Beuron and A. Hoenger |
| Summary |
It is now clear that kinesin-like-proteins (KLP), generally thought to be responsible for the transport of cellular cargoes, are involved in many different cellular processes now being widely investigated. In Kinesin Protocols, Isabelle Vernos and a panel of hands-on experts present their most productive and reproducible techniques for the identification, purification, and characterization of the kinesin superfamily of microtubule-dependent motors. The methods range from the most basic to the most sophisticated and include step-by-step instructions and extensive cautionary notes to ensure experimental success. Among the approaches discussed are methods to express and purify kinesins in different systems, to characterize microtubule-enhanced ATPase activity and motility properties, and to test microtubule destabilizing activity. Detailed examples of how to address functional studies are also presented, along with some very new methods for studying the role of KLP in the organization of microtubules in three dimensions. There are also advanced methods for the study of kinesins at the structural level. Comprehensive and highly practical, Kinesin Protocols makes available all the key basic and cutting-edge methods needed successfully to study the multifaceted world of kinesin-like proteins and to explore their many functions |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL |
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English |
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Print version record |
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digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL |
| In |
Springer Protocols |
| Subject |
Kinesin -- Laboratory manuals
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Kinesins -- isolation & purification
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Kinesins -- physiology
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SCIENCE -- Life Sciences -- Biochemistry.
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Kinesin
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Wetenschappelijke technieken.
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| Genre/Form |
Handbooks and manuals
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Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Vernos, Isabelle.
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| ISBN |
1592590691 |
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9781592590698 |
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0896037665 |
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9780896037663 |
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1280820608 |
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9781280820601 |
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9786610820603 |
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6610820600 |
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