Wiley Series on Mass Spectrometry; Title Page; Copyright; Table of Contents; List of Contributors; Introduction; List of Abbreviations; Chapter 1: Mass Spectrometry Techniques for In Vivo Stable Isotope Approaches; 1.1 Introduction; 1.2 Nomenclature for Light-Stable Isotope Changes; 1.3 Mass Spectrometry Techniques; 1.4 Choice of Mass Spectrometric Techniques and Applications to Measure Isotopic Enrichments in Metabolic Studies; 1.5 Conclusion and Future Perspectives; References; Chapter 2: Stable Isotope Technology; 2.1 History; 2.2 Definition; 2.3 Safety
2.4 Stable Isotopes and Natural Abundances2.5 Stable Isotope Selection; 2.6 Single or Multiple Label Selection; 2.7 Precursor Model; 2.8 Simultaneous Infusion; 2.9 Infusion Techniques; 2.10 Steady State; 2.11 Pool Selection; 2.12 Pool Models; 2.13 Flux: Synthesis and Breakdown; 2.14 Nitrogen Balance; 2.15 Doubly Labeled Water Method; 2.16 Whole-body Protein Synthesis; 2.17 Specific Protein Synthesis; 2.18 Calculations; 2.19 Considerations and Drawbacks of Isotopic Tracers; 2.20 Conclusion; References; Chapter 3: Stable Isotopes in Nutritional and Pediatric Research; 3.1 Introduction
3.2 Ethical Aspects3.3 Applications of Stable Isotopes in Nutritional and Pediatric Research; 3.4 Conclusion; References; Chapter 4: Early-Life Nutrition and Stable Isotope Techniques; 4.1 Introduction; 4.2 Breast Milk versus Infant Formula; 4.3 Techniques to Monitor Milk Intake; 4.4 Body Composition in Term and Preterm Infants; 4.5 Amino Acid Requirement; 4.6 Clinical Applications; 4.7 Additional Applications; 4.8 Discussion; 4.9 Conclusion; 4.10 Future Perspectives; References; Chapter 5: Assessment of Amino Acid Requirement in Children Using Stable Isotopes; 5.1 Introduction
5.2 Nutrient Needs and Definitions5.3 Methods to Determine Requirements; 5.4 Isotopic Tracer Methods; 5.5 Existing Methods to Determine Amino Acid Requirement for Neonates; 5.6 Use of the IAAO Method in the Pediatric Population; 5.7 Necessity for Performing the Study; 5.8 Biochemistry; 5.11 Analysis and Calculations; 5.12 Results; 5.13 Statistical Analysis; 5.14 Discussion; 5.15 Conclusion; 5.16 Future Perspectives; References; Chapter 6: Metabolism of Glutamine, Citrulline, and Arginine; Stable Isotopes Analyzing the Intestinal-Renal Axis; 6.1 Introduction; 6.2 Biochemistry
6.3 Isotopic Model6.4 Study Design; 6.5 Mass Spectrometry Methods; 6.6 Clinical Applications; 6.7 Calculations; 6.8 Discussion and Future Perspectives; References; Chapter 7: Applications in Fat Absorption and Metabolism; 7.1 Introduction; 7.2 Biochemistry of Fat Absorption; 7.3 Isotope Model; 7.4 Study Design/Infusion Protocols; 7.5 Analytical Equipment; 7.6 Analytical Conditions; 7.7 Accuracy and Precision; 7.8 Calculations; 7.9 Clinical Applications; 7.10 Future Perspectives; References; Chapter 8: Materno-Fetal Lipid Kinetics; 8.1 Introduction
