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Book Cover
E-book
Author Lambert, David G

Title Calcium Signaling Protocols
Edition 2nd ed
Published Totowa, NJ Humana Press, 2005

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Description 1 online resource (372 pages)
Series Methods in molecular biology Calcium signaling protocols
Methods in molecular biology ; 312. 1064-3745
Contents General -- Fluorescent Measurement of [Ca2+]c -- Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2 -- Measurement of [Ca2+]i in Cell Suspensions Using Indo-1 -- Specialist Measurement Systems -- Confocal Microscopy -- Single-Cell and Subcellular Measurement of Intracellular Ca2+ Concentration -- Ratiometric Ca2+ Measurements Using the FlexStation® Scanning Fluorometer -- Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader -- Measurement of Ca2+ Channel Activity -- Measurement of Ca2+ Entry Using 45Ca2+ -- Measurement of [3H]PN200-110 and [125I]?-Conotoxin MVIIA Binding -- Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents -- Measurement of Ins(1,4,5,)P3 and Ca2+ Release From Intracellular Stores -- Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protocols in Permeabilized Cells -- Measurement of Inositol(1,4,5)Trisphosphate Using a Stereospecific Radioreceptor Mass Assay -- Measurement of Calcium Fluxes in Permeabilized Cells Using a 45Ca2+ Uptake and Release Assay -- Microinjection of myo-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adherent Cells -- Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators -- Specialist Measurement Techniques -- Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry -- Measurement of Calcium and Movement in Heart Cells -- Simultaneous Analysis of Intracellular pH and Ca2+ From Cell Populations -- Measurement of Cytosolic-Free Ca2+ in Plant Tissue -- Ca2+-Sensitive Targets -- Assay and Purification of Calmodulin-Dependent Protein Kinase -- Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase and Isozyme Separation -- Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1)
Summary Now extensively updated and expanded, Calcium Signaling Protocols, Second Edition continues the excellence of the highly regarded first edition. New to this edition are chapters on the electrophysiological measurement of Ca2+ channel activity, enhanced coverage of confocal microscopy, and practical tutorials on two of the most common industrial high-throughput machines, the Fluorometric Imaging Plate Reader (FLIPR), and the FlexStation. All experimental chapters provide readily reproducible techniques for measuring the many facets of Ca2+ signaling. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Calcium Signaling Protocols, Second Edition provides for novice and expert researchers alike both theoretical and practical information for the optimal design and performance of studies of intracellular Ca2+ regulation
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Calcium -- Research -- Methodology -- Laboratory manuals
Calcium channels -- Methodology -- Research
Cellular signal transduction.
Calcium -- Research -- Methodology
Calcium -- Laboratory manuals
Calcium channels -- Research -- Methodology
Cellular signal transduction -- Research -- Methodology
Alkaline earth metals.
Life sciences.
Blood coagulation factors.
Ion channels.
Fluorimetry.
Cell physiology.
Chemical elements.
Glycoproteins.
Metals.
Physical sciences.
Biological transport.
Calcium.
Methodology.
Physiology.
Pharmacokinetics.
Calcium channels.
Fluorescence spectroscopy.
Photometry.
Membrane proteins.
Carrier proteins.
Metabolism.
Inorganic compounds.
Proteins.
Biological products.
Metals, Alkaline Earth
Biological Science Disciplines
Investigative Techniques
Kinetics
Second Messenger Systems
Blood Coagulation Factors
Biochemical Phenomena
Ion Channels
Fluorometry
Cell Physiological Phenomena
Ion Transport
Spectrum Analysis
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Elements
Luminescent Measurements
Membrane Glycoproteins
Metals
Chemical Phenomena
Natural Science Disciplines
Biological Factors
Membrane Transport Proteins
Chemistry Techniques, Analytical
Biological Transport
Calcium
Methods
Physiology
Pharmacokinetics
Calcium Channels
Signal Transduction
Spectrometry, Fluorescence
Calcium Signaling
Photometry
Membrane Proteins
Carrier Proteins
Disciplines and Occupations
Phenomena and Processes
Metabolism
Inorganic Chemicals
Chemicals and Drugs
Proteins
Amino Acids, Peptides, and Proteins
biological sciences.
metal.
physical sciences.
calcium.
methodology.
physiology.
metabolism (biological concept)
protein.
elements (chemical substances)
Proteins
Physiology
Physical sciences
Photometry
Pharmacokinetics
Methodology
Metals
Metabolism
Membrane proteins
Life sciences
Ion channels
Inorganic compounds
Glycoproteins
Fluorimetry
Fluorescence spectroscopy
Chemical elements
Cell physiology
Carrier proteins
Calcium channels
Blood coagulation factors
Biological transport
Alkaline earth metals
Cellular signal transduction -- Research -- Methodology
Calcium
Cellular signal transduction
Genre/Form Laboratory manuals
Form Electronic book
Author Lambert, David G, Contributor
ISBN 1280358890
9781280358890
9786610358892
6610358893
1592599494
9781592599493
1588294420
9781588294425
Other Titles Calcium Signaling Protocols, Second Edition