| Description |
1 online resource (xi, 234 pages) |
| Series |
Methods in molecular biology, 1064-3745 ; 865 |
|
Methods in molecular biology (Clifton, N.J.) ; 865
|
| Contents |
Influenza Viruses : an introduction / Yoshihiro Kawaoka and Gabriele Neumann -- Influenza virus isolation / Scott Krauss, David Walker, and Robert G. Webster -- Influenza virus titration, antigenic characterization, and serological methods for antibody detection / Alexander Klimov . [and others] -- Diagnosis of influenza virus / Kirsten St. George -- Solid-phase assays of receptor-binding specificity / Mikhail N. Matrosovich and Alexandra S. Gambaryan -- Chemiluminescent neuraminidase inhibition assay : a functional method for detection of influenza virus resistance to the neuraminidase inhibitors / Margaret Okomo-Adhiambo, Aeron C. Hurt, and Larisa V. Gubareva -- Fluorescence neuraminidase inhibition assay : a functional method for detection of influenza virus resistance to the neuraminidase inhibitors / Aeron C. Hurt, Margaret Okomo-Adhiambo, and Larisa V. Gubareva -- Animal models / Edwin J.B. Veldhuis Kroeze, Thijs Kuiken, and Albert D.M.E. Osterhaus -- Influenza virus surveillance, vaccine strain selection, and manufacture / Klaus Stöhr [and others] -- Genetic engineering of live attenuated influenza viruses / Hong Jin [and others] -- Influenza A virus molecular virology techniques / Bin Zhou and David E. Wentworth -- Reverse genetics of influenza viruses / Gabriele Neumann, Makoto Ozawa, and Yoshihiro Kawaoka -- Genetic analysis / Gavin J.D. Smith, Justin Bahl, and Dhanasekaran Vijaykrishna |
| Summary |
Reports of influenza-like illnesses date back to the Middle Ages, and outbreaks of influenza likely afflicted humans long before that. Over the last half century, influenza virus research has led to the development of two classes of antivirals - ion channel and neuraminidase inhibitors. Recently, a method of the artificial generation of an influenza virus was established. This system has been instrumental in the development of novel influenza vaccines and in the understanding of viral pathogenicity and the functions of viral proteins. Influenza Virus: Methods and Protocols summarizes the current techniques that have made this progress possible, ranging from protocols for virus isolation, growth, and subtyping to procedures for the efficient generation of any influenza virus. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Influenza Virus: Methods and Protocols seeks to serve both professionals and novices with the techniques used in numerous laboratories around the world that are, thus, the building blocks that underpin almost all influenza virus research |
| Analysis |
Medicine |
|
Medical virology |
|
Emerging infectious diseases |
|
Medicine & Public Health |
|
Infectious Diseases |
|
Virology |
|
geneeskunde |
|
volksgezondheid |
|
public health |
|
virologie |
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infectieziekten |
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Medicine (General) |
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Geneeskunde (algemeen) |
| Notes |
Includes index |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
| Subject |
Influenza viruses -- Laboratory manuals
|
|
Communicable diseases.
|
|
Influenza.
|
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Influenza vaccines.
|
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Human beings.
|
|
Humans
|
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Communicable Diseases
|
|
Influenza, Human
|
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Influenza Vaccines
|
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Orthomyxoviridae Infections
|
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Homo sapiens (species)
|
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Influenza vaccines
|
|
Influenza
|
|
Communicable diseases
|
|
Influenza viruses
|
| Genre/Form |
Laboratory manuals
|
|
Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Kawaoka, Yoshihiro, 1955-
|
|
Neumann, Gabriele
|
| ISBN |
9781617796210 |
|
1617796212 |
|
1617796204 |
|
9781617796203 |
|
