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Title Immunological tolerance : methods and protocols / edited by Ashleigh S. Boyd
Published New York : Humana Press : Springer, [2019]


Description 1 online resource (xi, 240 pages) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; volume 1899
Methods in molecular biology (Clifton, N.J.) ; v. 1899. 1064-3745
Contents Isolation and characterisation of human adipose-derived stem cells / Anna Wilson, Melisse Chee, Peter Butler, and Ashleigh S. Boyd -- Isolation of murine hematopoietic stem cells / Juan Bautista Menendez-Gonzalez, Lubaid Saleh, Gui Jie Feng, and Neil P. Rodrigues -- Generation of antigen-specific T cells from human induced pluripotent stem cells / Toshinobu Nishimura and Hiromitsu Nakauchi -- Isolation of human regulatory T lymphocytes by fluorescence-activated cell sorting / Kate Milward, Joanna Hester, and Kathryn J. Wood -- Identification and isolation of regulatory B cells in mouse and human / Madhvi Menon, Elizabeth C. Rosser, and Claudia Mauri -- Innovative standard operation procedure for isolating GMP-grade CD4+CD25+ T cells from non-mobilized leukapheresis / Wei Zhang, Suzanne M. Watt, and David J. Roberts -- Measuring CTLA-4-dependent suppressive function in regulatory T cells / Tie Zheng Hou, Omar S. Qureshi, and David M. Sansom -- Alloanergization method for inducing allospecific hyporesponsiveness in PBMC exposed to allostimulation in vitro in the context of costimulatory molecule blockade / Christine M. Barbon, Kenneth J. Janec, Rowan H. Kelner, James E. Norton, and Eva C. Guinan -- In vitro suppression of CD4+ T-cell responses by murine and human myeloid-derived suppressor cells / Katerina Vlachou and Panayotis Verginis -- Disassembling and reaggregating the thymus : the pros and cons of current assays / Elia Piccinini and Paola Bonfanti -- Isolation of highly viable thymic epithelial cells for use in in vitro and in vivo experiments / Mi-Jeong Kim and Thomas Serwold -- Immune tolerance and rejection in organ transplantation / Jessica Stolp, Masaaki Zaitsu, and Kathryn J. Wood -- Method of generating tolerogenic maturation-resistant dendritic cells and testing for their immune-regulatory functions in vivo in the context of transplantation / Sherrie J. Divito and Adrian E. Morelli -- Luminex-coupled EliFACS : a multiparametric method to enumerate and functionally characterize antigen-specific T cells in human peripheral blood / HuiJuan Lv, Khadir Raddassi, and Myra A. Lipes -- Clinical and functional evaluation of ocular inflammatory disease using the model of experimental autoimmune uveitis / Jun Chen and Rachel R. Caspi -- Methods of isolation and analysis of TREG immune infiltrates from injured and dystrophic skeletal muscle / Dalia Burzyn, Amy Wagers, Diane Mathis, and Massimiliano Cerletti
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed January 17, 2019)
Subject Immunological tolerance -- Laboratory manuals
Immune system.
Immunologic Techniques
Immune System Phenomena
Immune system
Immunological tolerance
Genre/Form Electronic books
Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Boyd, Ashleigh S., editor
ISBN 9781493989386