| Description |
1 online resource (xi, 240 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1899 |
|
Methods in molecular biology (Clifton, N.J.) ; v. 1899. 1064-3745
|
| Contents |
Isolation and characterisation of human adipose-derived stem cells / Anna Wilson, Melisse Chee, Peter Butler, and Ashleigh S. Boyd -- Isolation of murine hematopoietic stem cells / Juan Bautista Menendez-Gonzalez, Lubaid Saleh, Gui Jie Feng, and Neil P. Rodrigues -- Generation of antigen-specific T cells from human induced pluripotent stem cells / Toshinobu Nishimura and Hiromitsu Nakauchi -- Isolation of human regulatory T lymphocytes by fluorescence-activated cell sorting / Kate Milward, Joanna Hester, and Kathryn J. Wood -- Identification and isolation of regulatory B cells in mouse and human / Madhvi Menon, Elizabeth C. Rosser, and Claudia Mauri -- Innovative standard operation procedure for isolating GMP-grade CD4+CD25+ T cells from non-mobilized leukapheresis / Wei Zhang, Suzanne M. Watt, and David J. Roberts -- Measuring CTLA-4-dependent suppressive function in regulatory T cells / Tie Zheng Hou, Omar S. Qureshi, and David M. Sansom -- Alloanergization method for inducing allospecific hyporesponsiveness in PBMC exposed to allostimulation in vitro in the context of costimulatory molecule blockade / Christine M. Barbon, Kenneth J. Janec, Rowan H. Kelner, James E. Norton, and Eva C. Guinan -- In vitro suppression of CD4+ T-cell responses by murine and human myeloid-derived suppressor cells / Katerina Vlachou and Panayotis Verginis -- Disassembling and reaggregating the thymus : the pros and cons of current assays / Elia Piccinini and Paola Bonfanti -- Isolation of highly viable thymic epithelial cells for use in in vitro and in vivo experiments / Mi-Jeong Kim and Thomas Serwold -- Immune tolerance and rejection in organ transplantation / Jessica Stolp, Masaaki Zaitsu, and Kathryn J. Wood -- Method of generating tolerogenic maturation-resistant dendritic cells and testing for their immune-regulatory functions in vivo in the context of transplantation / Sherrie J. Divito and Adrian E. Morelli -- Luminex-coupled EliFACS : a multiparametric method to enumerate and functionally characterize antigen-specific T cells in human peripheral blood / HuiJuan Lv, Khadir Raddassi, and Myra A. Lipes -- Clinical and functional evaluation of ocular inflammatory disease using the model of experimental autoimmune uveitis / Jun Chen and Rachel R. Caspi -- Methods of isolation and analysis of TREG immune infiltrates from injured and dystrophic skeletal muscle / Dalia Burzyn, Amy Wagers, Diane Mathis, and Massimiliano Cerletti |
| Summary |
This edition provides a detailed, up-to-date overview of methods used in the field of immune tolerance. Chapters guide readers through tolerogenic cell types, isolating tolerogenic cell populations for study and therapeutic utility, multiple methods to study the mechanisms underpinning tolerance, methods to induce tolerance through thymus progenitors, and methods to assess the breakdown of immune tolerance in specific pathological conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunological Tolerance: Methods and Protocols aims to ensure successful results in the further study of this vital field |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from PDF title page (SpringerLink, viewed January 17, 2019) |
| Subject |
Immunological tolerance -- Laboratory manuals
|
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Immune system.
|
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Immunologic Techniques
|
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Immune System Phenomena
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Immune system
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Immunological tolerance
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| Genre/Form |
Electronic books
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Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Boyd, Ashleigh S., editor
|
| ISBN |
9781493989386 |
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1493989383 |
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