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Title The immune synapse : methods and protocols / edited by Cosima T. Baldari, Michael L. Dustin
Published New York : Humana Press : Springer, ©2017


Description 1 online resource (xiii, 588 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; 1584
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 1584
Springer protocols (Series)
Contents Immune synapse : past, present, and future / Michael L. Dustin and Cosima T. Baldari -- Analyzing actin dynamics at the immunological synapse / Katarzyna I. Jankowska and Janis K. Burkhardt -- Analysis of microtubules and microtubule-organizing center at the immune synapse / Noelia Blas-Rus, Eugenio Bustos-Morán, Francisco Sánchez-Madrid, and Noa B. Martín-Cófreces -- Analyzing the dynamics of signaling microclusters / Akiko Hashimoto-Tane, Tadashi Yokosuka, and Takashi Saito -- Reconstitution of TCR signaling using supported lipid bilayers / Xiaolei Su, Jonathon A. Ditlev, Michael K. Rosen, and Ronald D. Vale -- Plasma membrane sheets for studies of B cell antigen internalization from immune synapses / Carla R. Nowosad and Pavel Tolar -- Studying the dynamics of TCR internalization at the immune synapse / Enrique Calleja, Balbino Alarcón, and Clara L. Oeste -- T cell receptor activation of NF-?B in effector T cells : visualizing signaling events within and beyond the cytoplasmic domain of the immunological synapse / Maria K. Traver, Suman Paul, and Brian C. Schaefer -- Imaging vesicular traffic at the immune synapse / Jérôme Bouchet, Iratxe del Río-Iñiguez, and Andrés Alcover -- Analysis of TCR/CD3 recycling at the immune synapse / Laura Patrussi and Cosima T. Baldari -- Simultaneous membrane capacitance measurements and TIRF microscopy to study granule trafficking at immune synapses / Marwa Sleiman, David R. Stevens, and Jens Rettig -- Mathematical modeling of synaptic patterns / Anastasios Siokis, Philippe A. Robert, and Michael Meyer-Hermann -- Super-resolution analysis of TCR-dependent signaling : single-molecule localization microscopy / Valarie A. Barr, Jason Yi, and Lawrence E. Samelson -- Förster resonance energy transfer to study TCR-pMHC interactions in the immunological synapse / Gerhard J. Schütz and Johannes B. Huppa -- Two-dimensional analysis of cross-junctional molecular interaction by force probes / Lining Ju, Yunfeng Chen, Muaz Nik Rushdi, Wei Chen, and Cheng Zhu -- Studying dynamic plasma membrane binding of TCR-CD3 chains during immunological synapse formation using donor-quenching FRET and FLIM-FRET / Etienne Gagnon, Audrey Connolly, Jessica Dobbins, and Kai W. Wucherpfennig -- Revealing the role of microscale architecture in immune synapse function through surface micropatterning / Joung-Hyun Lee and Lance C. Kam -- Spatial control of biological ligands on surfaces applied to T cell activation / Haogang Cai, David Depoil, James Muller, Michael P. Sheetz, Michael L. Dustin, and Shalom J. Wind -- Probing synaptic biomechanics using micropillar arrays / Weiyang Jin, Charles T. Black, Lance C. Kam, and Morgan Huse -- Microchannels for the study of T cell immunological synapses and kinapses / Hélène D. Moreau, Philippe Bousso, and Ana-Maria Lennon-Duménil -- Purification of LAT-containing membranes from resting and activated T lymphocytes / Claire Hivroz, Paola Larghi, Mabel Jouve, and Laurence Ardouin -- Quantitative phosphoproteomic analysis of T-cell receptor signaling / Nagib Ahsan and Arthur R. Salomon -- Imaging asymmetric T cell division / Mirren Charnley and Sarah M. Russell -- Ultrastructure of immune synapses / Jaime Llodrá -- Systems imaging of the immune synapse / Rachel Ambler, Xiangtao Ruan, Robert F. Murphy, and Christoph Wülfing -- Comprehensive analysis of immunological synapse phenotypes using supported lipid bilayers / Salvatore Valvo, Viveka Mayya, Elena Seraia, Jehan Afrose, Hila Novak-Kotzer, Daniel Ebner, and Michael L. Dustin -- Studying immunoreceptor signaling in human T cells using electroporation of in vitro transcribed mRNA / Omkar Kawalekar, Carl H. June, and Michael C. Milone -- Protein expression toolkit for studying signaling in T cells / Ana Mafalda Santos, Jiandong Huo, Deborah Hatherley, Mami Chirifu, and Simon J. Davis -- Imaging the effector CD8 synapse / Gordon L. Frazer, Yukako Asano, and Gillian M. Griffiths -- Mast cell antibody-dependent degranulatory synapse / Salvatore Valitutti, Régis Joulia, and Eric Espinosa -- Measurement of lytic granule convergence after formation of an NK cell immunological synapse / Hsiang-Ting Hsu, Alexandre F. Carisey, and Jordan S. Orange -- Studying the T cell-astrocyte immune synapse / George P. Cribaro, Elena Saavedra-López, Paola V. Casanova, Laura Rodríguez, and Carlos Barcia -- Aberrant immunological synapses driven by leukemic antigen-presenting cells / Fabienne McClanahan Lucas and John G. Gribben -- Studying the immune synapse in HIV-1 infection / Iratxe del Río-Iñiguez, Jérôme Bouchet, and Andrés Alcover -- In vivo imaging of T cell immunological synapses and kinapses in lymph nodes / Hélène D. Moreau and Philippe Bousso -- Studying dendritic cell-T cell interactions under in vivo conditions / Nicholas van Panhuys
Bibliography Includes bibliographical references and index
Notes Online resource; title form PDF title page (SpringerProtocols, viewed March 6, 2017)
Subject Neuroimmunology -- Laboratory manuals
Synapses -- Laboratory manuals
Immunological Synapses
Medical -- Immunology.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Baldari, Cosima T., editor
Dustin, Michael L., editor
ISBN 9781493968817