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Book Cover
E-book

Title Cell cycle control and dysregulation protocols : cyclins, cyclin-dependent kinases, and other factors / edited by Antonio Giordano, Gaetano Romano
Published Totowa, N.J. : Humana Press, ©2004

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Description 1 online resource (xiii, 185 pages) : illustrations (some color)
Series Methods in molecular biology, 1064-3745 ; 285
Methods in molecular biology (Clifton, N.J.) ; v. 285
Contents The biology of cyclins and cyclin-dependent protein kinases: an introduction / Lucio Miele -- In situ immunofluorescence analysis: immunofluorescence microscopy / Amjad Javed [and others] -- In situ immunofluorescence analysis: analyzing RNA synthesis by 5-bromouridine-5'-triphosphate labeling / Amjad Javed [and others] -- Immunofluorescence analysis using epitope-tagged proteins: in vitro system / Amjad Javed [and others] -- Analysis of in vivo gene expression using epitope-tagged proteins / Amjad Javed [and others] -- Chromatin immunoprecipitation / Amjad Javed [and others]
Protein/deoxyribonucleic acid interactions linked to gene expression: electrophoretic mobility shift assay / Amjad Javed [and others] -- Protein/deoxyribonucleic acid interactions linked to gene expression: DNase I digestion / Amjad Javed [and others] -- Protein/deoxyribonucleic acid interactions linked to gene expression: ligation-mediated polymerase chain reaction / Amjad Javed [and others] -- Assays for cyclin-dependent kinase inhibitors / Adrian M. Senderowicz and Tyler Lahusen -- Protein degradation via the proteosome / Henry Hoff, Hong Zhang, and Christian Sell
The transformed phenotype / Henry Hoff [and others] -- A morphologic approach to detect apoptosis based on electron microscopy / Martyn K. White, Timothy G. Schneider, and Caterina Cinti -- Detection of apoptotic deoxyribonucleic acid break by in situ / Nick Translation [and others] -- Induction of deoxyribonucleic acid damage by alkylating agents / Salvatore Cortellino [and others] -- Induction of deoxyribonucleic acid damage by -- irradiation / Salvatore Cortellino, David P. Turner and Alfonso Bellacosa -- Ultraviolet irradiation of cells / David P. Turner, Anthony T. Yeung, and Alfonso Bellacosa -- Transient production of retroviral- and lentiviral-based vectors for the transduction of mammalian cells / Tiziana Tonini [and others]
Determination of functional viral titer by drug-resistance -- Colony assay, expression of green fluorescent protein, and '-galactoside staining / Tiziana Tonini [and others] -- Retroviral and lentiviral vector titration by the analysis of the activity of viral reverse transcriptase / Tiziana Tonini [and others] -- Single and double colloidal gold labeling in postembedding immunoelectron microscopy / Nicoletta Zini [and others] -- Multifluorescence labeling and colocalization analyses / Massimo Riccio [and others]
Summary The dysregulation of the normal cell cycle underlies an enormous number of pathological conditions, including the neurodegenerative disorders, all cancers, and diabetes. In Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors, expert laboratorians detail emerging methodologies for studying the cell cycle, its kinases, and kinase inhibitors. The authors focus on issues of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Many of the protocols are new, such as electron microsocopy to detect apoptosis and proteosome-mediated degradation, whereas others study the interactions between protein-protein, protein-DNA, and protein-RNA. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors offers pathbreaking researchers powerful tools for probing the cell cycle regulation across a wide variety of pathologies
Bibliography Includes bibliographical references and index
Subject Cell cycle -- Laboratory manuals
Cyclin-dependent kinases -- Laboratory manuals
Cyclins -- Laboratory manuals
Biology.
Immunohistochemistry.
Immunocytochemistry.
Nucleic acids.
Proteins.
Immunodiagnosis.
Life sciences.
Protein kinases.
Physical sciences.
Histology -- Technique.
Diagnosis.
Medical laboratory technology.
Phosphotransferases.
Transferases.
Enzymes.
DNA.
Gene expression.
Fluorescent antibody technique.
Cyclin-dependent kinases.
Biology
Immunohistochemistry
Nucleic Acids
Protein Serine-Threonine Kinases
Genetic Phenomena
Proline-Directed Protein Kinases
Proteins
Peptides
Immunologic Tests
Amino Acids, Peptides, and Proteins
Biological Science Disciplines
Protein Kinases
Histocytochemistry
Nucleic Acids, Nucleotides, and Nucleosides
Chemicals and Drugs
Natural Science Disciplines
Immunologic Techniques
Phenomena and Processes
Histological Techniques
Laboratory Techniques and Procedures
Phosphotransferases (Alcohol Group Acceptor)
Diagnosis
Clinical Laboratory Techniques
Phosphotransferases
Investigative Techniques
Disciplines and Occupations
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Transferases
Enzymes
Enzymes and Coenzymes
Cell Cycle Proteins
DNA
Gene Expression
Fluorescent Antibody Technique
Cyclin-Dependent Kinases
Intracellular Signaling Peptides and Proteins
Genetics
biology.
protein.
biological sciences.
physical sciences.
diagnosis.
enzyme.
SCIENCE -- Life Sciences -- Developmental Biology.
Transferases
Proteins
Protein kinases
Physical sciences
Phosphotransferases
Nucleic acids
Medical laboratory technology
Life sciences
Immunohistochemistry
Immunodiagnosis
Immunocytochemistry
Histology -- Technique
Gene expression
Fluorescent antibody technique
Enzymes
DNA
Diagnosis
Biology
Cell cycle
Cyclin-dependent kinases
Cyclins
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Giordano, Antonio, MD.
Romano, Gaetano, 1959-
LC no. 2004006931
ISBN 0896039498
9780896039490
1592598226
9781592598229