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Book Cover
E-book

Title Allergy methods and protocols / edited by Meinir G. Jones and Penny Lympany
Published Totowa, NJ : Humana Press, ©2008

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Description 1 online resource (xi, 352 pages) : illustrations
Series Methods in molecular medicine ; 138
Methods in molecular medicine ; 138. 1543-1894
Contents Understanding of the molecular mechanisms of allergy / Meinir Jones -- T cell : primary culture from peripheral blood / Monika Raulf-Heimsoth -- Production of T-cell lines / Helga Kahlert -- Production of human T-cell clones / Adrienne Verhoef -- Mapping of human T-cell epitopes of allergens / Thomas Zeiler and Tuomas Virtanen -- Determining MHC restriction of T-cell responses / Mark Larché -- Short-term culture of CD8 cells and intracellular cytokine staining / Beejal Vyas and Alistair Noble -- Isolation, flow cytometric analysis, and suppression assay of CD4+ CD25+ T-regulatory cells / Hayley Jeal -- Monocyte-derived dendritic cells as antigen-presenting cells in T-cell proliferation and cytokine production / Sun-sang J. Sung -- Ultrasensitive ELISA for measurement of human cytokine responses in primary culture / William P. Stefura [and others] -- Quantification of human chemokine production in TLR-stimulated and antigen-specific recall responses / Monique Stinson [and others] -- Standardization of allergen extracts / Jørgen Nedergaard Larsen and Sten Dreborg -- Immunoelectrophoresis for the characterization of allergen extracts / Gitte Nordskov Hansen and Jørgen Nedergaard Larsen -- Conjugation of haptens / Ranulfo Lemus and Meryl H. Karol -- Monoclonal antibodies / Helga Kahlert and Oliver Cromwell -- Purification of antibodies / Per H. Larsson -- Collection of air samples to quantify exposure to airborne allergens / Susan Gordon -- Assay of air sample eluates / Anne Renström and Susan Gordon -- The halogen assay : a new technique for measuring airborne allergen / Euan Tovey [and others] -- Measurement of specific IgG anti-Fel d 1 antibodies / Meinir G. Jones -- The facilitated antigen binding (FAB) assay : a protocol to measure allergen-specific inhibitory antibody activity / James N. Francis -- Microscopic identification and purity determination of pollen grains / Magdalena Rahl -- Biopanning for the characterization of allergen mimotopes / Isabella Pali-Schöll and Erika Jensen-Jarolim -- Identification of mast cells and mast cell subpopulations / Mark Buckley and Andrew F. Walls -- Purification and characterization of mast cell tryptase and chymase from human tissues / Alan R. McEuen and Andrew F. Walls -- Experimental activation of mast cells and their pharmacological modulation / Shaoheng He and Andrew F. Walls -- In situ hybridization / Kayhan T. Nouri-Aria
Summary In recent years, allergy research has focused on the causes and mechanisms of allergy. In parallel, there is also an impetus to try to understand mechanisms of natural tolerance and immunotherapy where allergy is being dampened. In Allergy: Methods and Protocols a groundbreaking new title from the Methods in Molecular Medicine series, leaders in the field provide assistance for researchers to gain insight into the molecular mechanisms involved in allergy by featuring an array of protocols. These cover a range of disciplines including allergy, immunology, cell biology and histology and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, T regulatory cells. Techniques discussed include; B and T cell epitope mapping, characterization of allergens, conjugation of haptens, preparation of monoclonal antibodies, collection and sampling of airborne allergens, IgG antibodies and facilitated antigen blocking assays, identification and purification of mast cells and in situ hybridisation. Allergy: Methods and Protocols will be a remarkably useful bench tool for anyone embarking in or continuing with their research in allergy
Analysis allergies
overgevoeligheid
hypersensitivity
immuniteitsreactie
immune response
allergenen
allergens
protocollen
protocols
Human Allergies
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Allergy -- Laboratory manuals
Allergy.
Immunologic diseases.
Diseases.
Laboratory Manuals
Hypersensitivity
Immune System Diseases
Publication Formats
Disease
Publication Characteristics
Immunologic diseases
Diseases
Allergy
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Jones, Meinir G.
Lympany, Penny.
ISBN 1597453668
9781597453660