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Book Cover
E-book

Uniform Title Immunohistochemistry and immunocytochemistry (Del Valle)
Title Immunohistochemistry and immunocytochemistry : methods and protocols / edited by Luis Del Valle
Published New York, NY : Humana Press, [2022]

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Description 1 online resource (1 PDF (x, 286 pages)) : illustrations (some color)
Series Methods in molecular biology, 1940-6029 ; [2422]
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 2422. 1064-3745
Springer protocols (Series) 1949-2448
Contents Introduction to Immunohistochemistry. From Evolving Science to Timeless Art -- Immunohistochemistry in Historical Perspective. Knowing the Past to Understand the Present -- Sample Preparation & Fixation for Histology and Pathology -- Tissue Processing -- Antigen Retrieval and Signal Amplification -- Primary Antibody Selection and Blocking Techniques for Immunohistochemistry -- Probes for Fluorescent Visualization of Specific Cellular Organelles -- Enzymatic Immunohistochemistry -- Fluorescent Immunohistochemistry -- Double Labeling Fluorescent Immunocytochemistry -- Multiplexing and Spectral Microscopy -- In situ Hybridization (Fluorescent and Enzymatic) -- Proximity Ligation Assay (PLA) -- Immunohistochemistry, Quality control and Principles of Validation in the Central Nervous System -- Culture and Phenotyping of Glial Cell Cultures, Gliospheres and Neurospheres -- ER+ Breast Cancer Mammosphere Formation and Analysis -- Detection of Circulating and Tissue Myeloid-Derived Suppressor Cells (MDSCs) by Flow Cytometry -- Comet Assay for the Detection of Single and Double Strand DNA Breaks -- Fluorescent-Activated Cell Sorting (Flow Cytometry)
Summary This volume provides a comprehensive reference guide for researchers to study the applications of labeled antibodies. Chapters guide reader through the the and practice of immunohistochemistry, immunocytochemistry and immunofluorescence techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and useful tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Immunohistochemistry and Immunofluorescence: Methods and Protocols aims to be a useful practical guide to scientists to help further their study in this field
Bibliography Includes bibliographical references and index
Notes Description based on online resource; title from PDF title page (SpringerLink, viewed June 10, 2022)
Subject Immunohistochemistry.
Immunocytochemistry.
Immunohistochemistry -- methods
Immunohistochemistry
Immunocytochemistry
Immunohistochemistry
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Del Valle, Luis (Of Louisiana State University Health Sciences Center), editor.
ISBN 9781071619483
1071619489