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Title Antibody engineering protocols / edited by Sudhir Paul
Published Totowa, N.J. : Humana Press, [1995]


Location Call no. Vol. Availability
 W'PONDS  616.0793 Pau/Aep  AVAILABLE
Description xii, 450 pages : illustrations ; 23 cm
Series Methods in molecular biology ; v. 51
Methods in molecular biology (Clifton, N.J.) ; v. 51
Contents Machine derived contents note: SEQHUNT: A Program to Screen Aligned Nucleotide and Amino Acid Sequences. Molecular Modeling of Antibody-Combining Sites. Structure and Properties of Human Immunoglobulin Light-Chain Dimers. Crystallographic and Chromatographic Methods for Study of Antibody Light Chains and Other Proteins. Detection of Human Variable Gene Family Expression at the Single-Cell Level. Purification of Reduced and Alkylated Antibody Subunits. Murine Monoclonal Antibody Development. Comparative Properties of Polyclonal and Monoclonal Antibodies. Evaluation of Antibody Clonality. Affinity Immunoblotting. Epitope and Idiotope Mapping Using Monoclonal Antibodies. Anti-Idiotypic Antibodies That Mimic Opioids. Catalytic Antibodies: Structure and Possible Applications. Preparation and Assay of Acetylcholinesterase Antibody. DNA Hydrolysis by Antibodies. Screening Strategies for Catalytic Antitransition-State Analog Antibodies. Expression of Chimeric Immunoglobulin Genes in Mammalian Cells. Single-Chain Anti-DNA Fv. Molecular Cloning of Antiground-State Proteolytic Antibody Fragments. Cloning and Bacterial Expression of an Esterolytic sFv. Site-Directed Mutagenesis of Antibody-Variable Regions. Synthetic Antibody Gene Libraries for In Vitro Affinity Maturation. Chaperonins in Phage Display of Antibody Fragments. Phage-Display Libraries of Murine and Human Antibody Fab Fragments. Selection of Human Immunoglobulin Light Chains from a Phage-Display Library. Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography. Rapid Purification of Recombinant Antibody Fragments for Catalysis Screening. Assay of Radiolabeled VIP Binding and Hydrolysis by Antibodies. Methods of Measuring Thyroglobulin and Peptide-Methylcoumarinamide Hydrolysis by Autoantibodies. Radiolabeling of Antibodies for Therapy and Diagnosis. Index
Bibliography Includes bibliographical references and index
Notes Also available online via the World Wide Web, by subscription to (BMP) via Humana Press
Subject Immunoglobulins -- Biotechnology.
Protein engineering.
Author Paul, Sudhir.
Humana Press.
LC no. 95000423
ISBN 0896032752 (alk. paper)