| Description |
1 online resource (xii, 416 pages) : illustrations |
| Series |
Springer protocols |
|
Methods in molecular biology, 1940-6029 ; 588 |
|
Springer protocols (Series)
|
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Methods in molecular biology (Clifton, N.J.) ; v. 588
|
| Contents |
Overview of antibodies for immunochemistry / Constance Oliver and Maria Célia Jamur -- Introduction to the purification of antibodies / Ana Cristina Grodzki and Elsa Berenstein -- Antibody purification : ammonium sulfate fractionation or gel filtration / Ana Cristina Grodzki and Elsa Berenstein -- Antibody purification : ion-exchange chromatography / Ana Cristina Grodzki and Elsa Berenstein -- Antibody purification : affinity chromatography--Protein A and Protein G Sepharose / Ana Cristina Grodzki and Elsa Berenstein -- Conjugation of fluorochromes to antibodies / Su-Yau Mao and J. Michael Mullins -- Biotinylation of antibodies / Su-Yau Mao -- Cell fixatives for immunostaining / Maria Célia Jamur and Constance Oliver -- Permeabilization of cell membranes / Maria Célia Jamur and Constance Oliver -- Preparation of frozen sections for analysis / Gary L. Bratthauer -- Processing of cytological specimens / Gary L. Bratthauer -- Processing of tissue culture cells / Gary L. Bratthauer -- Processing of tissue specimens / Gary L. Bratthauer -- Heat induced antigen retrieval for immunohistochemical reactions in routinely processed paraffin sections / Laszlo Krenacs, Tibor Krenacs, Eva Stelkovics, and Mark Raffeld -- Fluorochromes : properties and characteristics / J. Michael Mullins -- Direct immunofluorescent labeling of cells / Maria Veronica Dávila Pástor -- Fluorescence labeling of surface antigens of attached or suspended tissue-culture cells / Mark C. Willingham -- Fluorescence labeling of intracellular antigens of attached or suspended tissue-culture cells / Mark C. Willingham -- Fluorescent visualization of macromolecules in Drosophila whole mounts / Ricardo Guelerman Pinheiro Ramos, Luciana Claudia Herculano Machado, and Livia Maria Rosatto Moda -- Overview of conventional fluorescence photomicrography / J. Michael Mullins -- Overview of confocal microscopy / William D. Swaim -- Overview of laser microbeam applications as related to antibody targeting / P. Scott Pine -- Immuno-laser capture microdissection of rat brain neurons for real time quantitative PCR / Denis G. Baskin and L. Scot Bastian -- Overview of antigen detection through enzymatic activity / Gary L. Bratthauer -- The peroxidase-antiperoxidase (PAP) method and other all-immunologic detection methods / Gary L. Bratthauer -- The avidin-biotin complex (ABC) method and other avidin-biotin binding methods / Gary L. Bratthauer -- Avidin-biotin labeling of cellular antigens in cryostat-sectioned tissue / Mark Raffeld and Elaine S. Jaffe -- Multiple antigen immunostaining procedures / Tibor Krenacs, Laszlo Krenacs, and Mark Raffeld -- Immunoenzymatic quantitative analysis of antigens expressed on the cell surface (cell-ELISA) / Elaine Vicente Lourenço, Maria-Cristina Roque-Barreira -- Use of immunogold with silver enhancement / Constance Oliver -- Overview of flow cytometry and fluorescent probes for flow cytometry / Robert E. Cunningham -- Tissue disaggregation / Robert E. Cunningham -- Indirect immunofluorescent labeling of viable cells / Robert E. Cunningham -- Indirect immunofluorescent labeling of fixed cells / Robert E. Cunningham -- Fluorescent labeling of DNA / Robert E. Cunningham -- Deparaffinization and processing of pathologic material / Robert E. Cunningham -- Fixation and embedding / Constance Oliver and Maria Célia Jamur -- Preparation of colloidal gold / Constance Oliver -- Conjugation of colloidal gold to proteins / Constance Oliver -- Colloidal gold/streptavidin methods / Constance Oliver -- Pre-embedding labeling methods / Constance Oliver -- Postembedding labeling methods / Constance Oliver -- The Clinical immunohistochemistry laboratory : regulations and troubleshooting guidelines / Patricia A. Fetsch and Andrea Abati |
| Summary |
Immunocytochemistry has significantly evolved in past years. Once a laborious technique with limited application, the introduction of new detection systems and instrumentation has facilitated the widespread use of immunocytochemical methods. In the post-genomic era, the use of immunocytochemical methods has grown to include the identification of a broad spectrum of antigens including proteins, carbohydrates and lipids from virtually every organism. In this book researchers explore the modern methods employed in the field today, now widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions, and to demonstrate the localization of subcellular components |
| Analysis |
immunocytochemie |
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immunocytochemistry |
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cytochemie |
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cytochemistry |
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immunochemie |
|
immunochemistry |
|
antilichamen |
|
antibodies |
|
ige |
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fluorescentiemicroscopie |
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fluorescence microscopy |
|
methodologie |
|
methodology |
|
protocollen |
|
protocols |
|
Biological Techniques |
|
Immunology |
|
Molecular Biology (General) |
|
Biologische technieken |
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Immunologie |
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Moleculaire biologie (algemeen) |
| Notes |
Title from PDF title page (SpringerLink, viewed January 6, 2010) |
| Bibliography |
Includes bibliographical references and index |
| Notes |
English |
| Subject |
Immunocytochemistry -- Laboratory manuals
|
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In situ hybridization -- Laboratory manuals
|
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Immunohistochemistry -- methods
|
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Immunohistochemistry -- standards
|
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Immunocytochemistry
|
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In situ hybridization
|
| Genre/Form |
Laboratory manuals
|
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Laboratory manuals.
|
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Manuels de laboratoire.
|
| Form |
Electronic book
|
| Author |
Oliver, Constance.
|
|
Jamur, Maria Célia.
|
| ISBN |
9781597453240 |
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1597453242 |
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9781607613787 |
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1607613786 |
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9781617796821 |
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1617796824 |
|
