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Title Immunocytochemical methods and protocols / edited by Lorette C. Javois
Edition 2nd ed
Published Totowa, NJ : Humana Press, ©1999


Description 1 online resource (xii, 465 pages) : illustrations
Series Methods in molecular biology ; v. 115
Methods in molecular biology (Clifton, N.J.) ; v. 115
Contents Part I. Antibody Preparation -- Overview of Antibody Use in Immunocytochemistry / Su-Yau Mao, Lorette C. Javois, and Ute M. Kent -- Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration / Ute M. Kent -- Purification of Antibodies Using Ion-Exchange Chromatography / Ute M. Kent -- Purification of Antibodies Using Affinity Chromatography / Ute M. Kent -- Purification of Antibodies Using Protein A-Sepharose and FPLC / Ute M. Kent -- Conjugation of Fluorochromes to Antibodies / Su-Yau Mao -- Biotinylation of Antibodies / Su-Yau Mao -- Part II. Tissue Preparation for light Microscopic Analyses -- Overview of Cell Fixatives and Cell Membrane Permeants / Melissa A. Melan -- Preparation of Frozen Sections for Analysis / Gary L. Bratthauer -- Processing of Cytological Specimens / Gary L. Bratthauer -- Processing of Tissue Culture Cells / Gary L. Bratthauer -- Processing of Tissue Specimens / Gary L. Bratthauer -- Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin Sections / Laszlo Krenacs, Tibor Krenacs, and Mark Raffeld -- Part III. Light Microscopic Detection Systems -- Overview of Fluorochromes / J. Michael Mullins -- Direct Immunofluorescent Labeling of Cells / Lorette C. Javois -- Fluorescence Labeling of Surface Antigens on Attached or Suspended Tissue-Culture Cells / Mark C. Willingham -- Fluorescence Labeling of Intracellular Antigens in Attached or Suspended Tissue-Culture Cells / Mark C. Willingham -- Fluorescent Labeling of Surface or Intracellular Antigens in Whole-Mounts / Lorette C. Javois -- TUNEL Assay for Apoptotic Cells / Virginia M. Heatwole -- Overview of Fluorescence Analysis with the Confocal Microscope / Liana Harvath -- Overview of Laser Microbeam Applications as Related to Antibody Targeting / P. Scott Pine -- Overview of Fluorescence Photomicography / Lorette C. Javois and J. Michael Mullins -- Overview of Antigen Detection Through Enzymatic Activity / Gary L. Bratthauer -- The Peroxidase-Antiperoxidase (PAP) Method and Other All-Immunologic Detection Methods / Gary L. Bratthauer -- The Avidin Biotin Complex (ABC) Method and Other Avidin Biotin Binding Methods / Gary L. Bratthauer -- Avidin Biotin Labeling of Cellular Antigens in Cryostat Sectioned Tissue / Mark Raffeld and Elaine S. Jaffe -- Multiple Antigen Immunostaining Procedures / Laszlo Krenacs, Tibor Krenacs, and Mark Raffeld -- ELISA on Attachment Dependent or Suspension Grown Cells / Julie Ann Brent -- Use of Immunogold with Silver Enhancement / Constance Oliver -- Part IV. Fluorescence Activated Cell Sorter (FACS) Analyses -- Overview of Flow Cytometry and Fluorescent Probes for Cytometry / Robert E. Cunningham -- Tissue Disaggregation/ Robert E. Cunningham -- Indirect Immunofluorescent Labeling of Viable Cells / Robert E. Cunningham -- Indirect Immunofluorescent Labeling of Fixed Cells / Robert E. Cunningham -- Fluorescent Labeling of DNA / Robert E. Cunningham -- Deparaffinization and Processing of Pathologic Material / Robert E. Cunningham -- Assay for Phagocytosis / Liana Harvath and Douglas A. Terle -- Assay for Filamentous Actin / Liana Harvath -- Assay for Chemoattractant Binding / Liana Harvath, Robert R. Aksamit, and Robert E. Cunningham -- Assay for Oxidative Metabolism / Liana Harvath and Douglas A. Terle -- Part V. Colloidal Gold Detection Systems For Electron Microscopic Analyses -- Fixation and Embedding / Constance Oliver and Maria Celia Jamur -- Preparation of Colloidal Gold / Constance Oliver -- Conjugation of Colloidal Gold to Proteins / Constance Oliver -- Colloidal Gold/Streptavidin Methods / Constance Oliver -- Pre-Embedding Labeling Methods / Constance Oliver -- Postembedding Labeling Methods / Constance Oliver -- Part VI. Applications For Nucleic Acid Localization By In Situ Hybridization -- Overview of In Situ Hybridization / Roland M. Nardone -- In Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase-Labeled Centromeric Probe / Roland M. Nardone -- Fluorescence In Situ Hybridization Using Whole Chromosome Library Probes / Roland M. Nardone -- In Situ RT-PCR and Hybridization Techniques / Vishakha Thaker -- Part VII. Special Applications For the Clinical Laboratory -- Overview of the Clinical Immunohistochemistry Laboratory : Regulations and Troubleshooting Guidelines / Patricia A. Fetsch and Andrea Abati -- Overview of Immunohistochemical Approaches to the Differential Diagnosis of Tumors / Dennis M. Frisman -- Overview of Automated Immunostainers / Gilbert E. Herman, Edna A. Elfont, and Alton D. Floyd
Summary In Animal Cell Biotechnology, scientists with long-refined expertise describe cutting-edge techniques for the production of therapeutic proteins and vaccines. Capturing the major advances that have occurred in both the science and the technology of these biopharmaceuticals, this important book covers the powerful new techniques used in genetically manipulating animal cells, optimizing their growth in defined media (particularly at large- scale), avoiding contamination, and in the harvesting and analysis of cell products. Topics include basic culture facilities and methods; molecular methods for gene transfection; cell immortalization and cell fusion; and techniques for the study of cell growth, viability, metabolism, and productivity. Animal Cell Biotechnology constitutes a comprehensive manual of state-of-the-art techniques for setting up a cell culture laboratory, maintaining cell lines, and optimizing critical parameters for cell culture. This authoritative book will significantly aid researchers in developing new monoclonal antibodies, vaccines, anticancer agents, and drugs for the widest spectrum of cardiovascular, respiratory, and immune diseases
Analysis biochemie
Biology (General)
Biologie (algemeen)
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Immunocytochemistry -- Laboratory manuals
In situ hybridization -- Laboratory manuals
Immunohistochemistry -- methods
Immunohistochemistry -- standards
SCIENCE -- Life Sciences -- Anatomy & Physiology.
In situ hybridization
Wetenschappelijke technieken.
Genre/Form Laboratory manuals
Gidsen (vorm)
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Javois, Lorette C
LC no. 98046930
ISBN 9781592592135