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Title Making and using antibodies : a practical handbook / edited by Gary C. Howard, Matthew R. Kaser
Published Boca Raton, FL : CRC Press/Taylor & Francis, ©2007


Description 1 online resource (394 pages, 2 unnumbered pages of plates) : illustrations (some color)
Contents Antibodies; Matthew R. Kaser and Gary C. Howard; ; Antigens; Paul Algate, Jory Baldridge, and Sally Mossman; ; Adjuvants; Jory Baldridge, Paul Algate, and Sally Mossman; ; Production of Polyclonal Antibodies; Lon V. Kendall; ; Production of Monoclonal Antibodies; Kathleen C.F. Sheehan; ; Quantitative Production of Monoclonal Antibodies; David A. Fox and Elizabeth M. Smith; ; Purification and Characterization of Antibodies; Joseph P; Chandler; ; Making Antibodies in Bacteria; Frederic A. Fellouse and Sachdev S. Sidhu; ; Chemical and Proteolytic Modification of Antibodies; George P. Smith; ; Applications; Lee Bendickson and Marit Nilsen-Hamilton; ; Immunohistochemical Methods; José A. Ramos-Vara and Julie Ackerman Saettele; ; Immunoelectron Microscopy; Sara E. Miller and David N. Howell; ; Flow Cytometry; Kristi R. Harkins and M. Elaine Kunze; ; ELISAs; John Chen and Gary C. Howard; ; Antibodies in the Future: Challenges and Opportunities; Matthew R. Kaser and Gary C. Howard
Summary Written for professionals in biomedical research, Making and Using Antibodies: A Practical Handbook presents methods for the production, characterization, and use of antibodies. It takes readers step-by-step from antigen preparation to immunizations, adjuvants, screening, purification, storage, and application. Introductory material preceding each chapter gives readers the necessary background. It covers basic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry
Bibliography Includes bibliographical references and index
Notes Print version record
Subject Immunoglobulins -- Handbooks, manuals, etc
Medical laboratory technology.
Antibody Formation
Clinical Laboratory Techniques
SCIENCE -- Life Sciences -- Anatomy & Physiology.
Medical laboratory technology
Genre/Form Laboratory manuals
Handbooks and manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Howard, Gary C
Kaser, Matthew R
LC no. 2006047535
ISBN 9781420005196