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Title Diagnostic and therapeutic antibodies / edited by Andrew J.T. George and Catherine E. Urch
Published Totowa, N.J. : Humana Press, ©2000

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Description 1 online resource (xiv, 477 pages) : illustrations
Series Methods in molecular medicine ; 40
Methods in molecular medicine ; 40. 1543-1894
Contents pt. 1. Introduction -- The antibody molecule / Andrew J.T. George -- Polyclonal and monoclonal antibodies / Mary A. Ritter -- Engineering antibody molecules / Rakesh Verma -- Phage display technology / Michael Johns -- pt. 2. Antibodies in medicine -- Prospects for the application of antibodies in medicine / Herman Waldmann -- Antibodies for neoplastic disease: solid tumors / Ian T.W. Matthews -- The application of monoclonal antibodies in the treatment of lymphoma / Martin J. Glennie -- Antibodies for inflammatory disease: effector cells / Richard Smith -- Antibodies for inflammatory disease: cytokines / Peter C. Taylor -- Antibodies for transplantation / Denise L. Faustman -- Antibody-based therapies in infectious diseases / H. Barbaros Oral -- Antibodies in nuclear medicine / A. Michael Peters -- Animal models for tumor localization / Gail Rowlinson-Busza -- Antibodies for immunoassays / David J. Newman -- pt. 3. Ethics and industry -- Intellectual property / William M. Brown -- From laboratory to clinic: the story of CAMPATH-1 / Geoff Hale -- pt. 4. Production and purification -- Production of monoclonal antibodies / Jóna Freysdóttir -- Purification of monoclonal antibodies using protein A/G / Bridget Heelan -- Preparation of monoclonal antibodies using ion exchange chromatography / Maureen Power -- Quality control of raw materials / Patrick Harrison -- Cell banks and stability of antibody production / Pru Bird -- Measurement of endotoxin / Jenny Phillips -- Aseptic vial filling / Kuldip Bhamra -- Measurement of antibody concentrations by hemagglutination / Jenny Phillips -- pt. 5. Clinical application of antibodies -- Enzymatic digestion of monoclonal antibodies / Sarah M. Andrew -- How to make bispecific antibodies / Ruth R. French -- Radiolabeling of monoclonal antibodies / Calvin S.R. Gooden -- Determination of the immunoreactivity of radiolabeled monoclonal antibodies / Gail Rowlinson-Busza -- Use of biosensors to measure the kinetics of antibody-antigen interactions / Andrew J.T. George -- pt. 6. Application of antibodies in vitro -- How to set up an ELISA / Bill Jordan -- Measurement of HAMA and anti-idiotypic antibodies / Steve Nicholson -- SDS-PAGE and Western blotting / Abdulhamid A. Al-Tubuly -- Flow cytometric analysis / Paul F. McKay -- Immunocytochemistry / Susan Van Noorden -- Immunolabeling for electron microscopy / Catherine E. Sarraf -- pt. 7. Antibody engineering -- PCR of the V region / Rakesh Verma -- Phage display technology: protocols / Michael Johns
Summary The use of monoclonal antibodies in treating disease has long held tantalizing promise, and the recent explosion of successful clinical trials-leading to the licensing of novel antibody-based therapeutic agents-has clearly demonstrated the power of recombinant antibody engineering, and greatly rejuvenated interest in their emergent clinical applications. In Diagnostic and Therapeutic Antibodies, a team of experts comprehensively reviews the theoretical and practical aspects of applying antibodies in both the laboratory and clinic. For general understanding, the book thoroughly introduces the basic science of the antibody molecule, including its structure and generation. Several novel in vivo applications of therapeutic antibodies are then presented to illustrate their special value in varied clinical settings. For those new to the field, there are readily reproducible methods for generating and purifying antibodies and for modifying them for clinical application, including how to measure their affinity and immunoactivity, and how to employ them in staining procedures and in a variety of in vitro assays. Timely and comprehensive, Diagnostic and Therapeutic Antibodies offers todays clinical researchers a concise introduction to the field, as well as a highly useful compendium of practical protocols that will greatly facilitate the application of these powerful new diagnostic and therapeutic agents
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. http://purl.oclc.org/DLF/benchrepro0212 MiAaHDL
English
Print version record
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
In Springer Protocols
Subject Monoclonal antibodies -- Therapeutic use
Immunoglobulins -- Therapeutic use
Immunotherapy.
Monoclonal antibodies -- Diagnostic use
Immunoglobulins.
Biological products -- Therapeutic use.
Therapeutics.
Blood proteins.
Globulins.
Proteins.
Monoclonal antibodies.
Methodology.
Antibodies
Investigative Techniques
Immunomodulation
Biological Therapy
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Immunoglobulins
Immunoproteins
Serum Globulins
Therapeutics
Blood Proteins
Globulins
Proteins
Amino Acids, Peptides, and Proteins
Chemicals and Drugs
Antibodies, Monoclonal
Immunotherapy
Methods
Immunoglobulins, Intravenous
protein.
methodology.
treating (health care function)
MEDICAL -- Pharmacology.
Therapeutics
Proteins
Monoclonal antibodies
Methodology
Immunoglobulins
Globulins
Blood proteins
Biological products -- Therapeutic use
Immunoglobulins -- Therapeutic use
Immunotherapy
Monoclonal antibodies -- Diagnostic use
Monoclonal antibodies -- Therapeutic use
Antikörper
Diagnostik
Therapie
Antistoffen.
Monoklonale antistoffen.
Immunotherapie.
Immunoglobulinen.
Diagnose.
Diagnostica.
Wetenschappelijke technieken.
Genre/Form Gidsen (vorm)
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author George, Andrew J. T.
Urch, Catherine E.
ISBN 9781592590766
1592590764
0585418780
9780585418780
9786610820665
661082066X
1280820667
9781280820663