Limit search to available items
Book Cover

Title Protein localization by fluorescence microscopy : a practical approach / edited by Victoria J. Allan
Published Oxford ; New York : Oxford University Press, ©2000


Description 1 online resource (xxiv, 231 pages) : illustrations (some color)
Series The practical approach series ; 218
Practical approach series ; 218.
Contents 1. Basic immunofluorescence / Victoria J. Allan -- 2. Immunofluorescent labelling of sections / J. Victor Small / Alison J. North -- 3. Simultaneous in situ detection of DNA and proteins / Klaus Ersfeld / Elisa M. Stone -- 4. Instruments for fluorescence imaging / W.B. Amos -- 5. Fluorescence microscopy of living vertebrate cells / David Shima / Rainer Pepperkok -- 6. Visualizing fluorescence in Drosophila -- optimal detection in thick specimens / Ilan Davis -- 7. Green fluorescent protein in plants / Chris Hawes / Petra Boevink / Ian Moore -- 8. Fluorescence microscopy in yeast / Iain M. Hagan / Kathryn R. Ayscough -- App. A1 Excitation and emission maxima of commonly used fluorophores -- App. A2 Commercial sources of antibodies -- App. A3 Optical units -- App. A4 List of Suppliers
Summary There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable "tricks of the trade" along the way. Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice whilst extending the range of the experienced user
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Proteins -- Analysis.
Fluorescence microscopy.
Green fluorescent protein.
Luminescent Proteins
Microscopy, Fluorescence
Sequence Analysis, Protein
fluorescence microscopy.
SCIENCE -- Life Sciences -- Biochemistry.
Proteins -- Analysis.
Fluorescence microscopy.
Green fluorescent protein.
Sequence Analysis, Protein.
Microscopy, Fluorescence.
Luminescent Proteins.
Fluorescence microscopy
Green fluorescent protein
Proteins -- Analysis
Form Electronic book
Author Allan, Victoria J
ISBN 0585483396