Title Cell cycle control and dysregulation protocols : cyclins, cyclin-dependent kinases, and other factors / edited by Antonio Giordano, Gaetano Romano

Published Totowa, N.J. : Humana Press, ©2004

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Description 1 online resource (xiii, 185 pages) : illustrations (some color) Series Methods in molecular biology, 1064-3745 ; 285 Methods in molecular biology (Clifton, N.J.) ; v. 285 Contents The biology of cyclins and cyclin-dependent protein kinases: an introduction / Lucio Miele -- In situ immunofluorescence analysis: immunofluorescence microscopy / Amjad Javed [and others] -- In situ immunofluorescence analysis: analyzing RNA synthesis by 5-bromouridine-5'-triphosphate labeling / Amjad Javed [and others] -- Immunofluorescence analysis using epitope-tagged proteins: in vitro system / Amjad Javed [and others] -- Analysis of in vivo gene expression using epitope-tagged proteins / Amjad Javed [and others] -- Chromatin immunoprecipitation / Amjad Javed [and others] Protein/deoxyribonucleic acid interactions linked to gene expression: electrophoretic mobility shift assay / Amjad Javed [and others] -- Protein/deoxyribonucleic acid interactions linked to gene expression: DNase I digestion / Amjad Javed [and others] -- Protein/deoxyribonucleic acid interactions linked to gene expression: ligation-mediated polymerase chain reaction / Amjad Javed [and others] -- Assays for cyclin-dependent kinase inhibitors / Adrian M. Senderowicz and Tyler Lahusen -- Protein degradation via the proteosome / Henry Hoff, Hong Zhang, and Christian Sell The transformed phenotype / Henry Hoff [and others] -- A morphologic approach to detect apoptosis based on electron microscopy / Martyn K. White, Timothy G. Schneider, and Caterina Cinti -- Detection of apoptotic deoxyribonucleic acid break by in situ / Nick Translation [and others] -- Induction of deoxyribonucleic acid damage by alkylating agents / Salvatore Cortellino [and others] -- Induction of deoxyribonucleic acid damage by -- irradiation / Salvatore Cortellino, David P. Turner and Alfonso Bellacosa -- Ultraviolet irradiation of cells / David P. Turner, Anthony T. Yeung, and Alfonso Bellacosa -- Transient production of retroviral- and lentiviral-based vectors for the transduction of mammalian cells / Tiziana Tonini [and others] Determination of functional viral titer by drug-resistance -- Colony assay, expression of green fluorescent protein, and '-galactoside staining / Tiziana Tonini [and others] -- Retroviral and lentiviral vector titration by the analysis of the activity of viral reverse transcriptase / Tiziana Tonini [and others] -- Single and double colloidal gold labeling in postembedding immunoelectron microscopy / Nicoletta Zini [and others] -- Multifluorescence labeling and colocalization analyses / Massimo Riccio [and others] Summary The dysregulation of the normal cell cycle underlies an enormous number of pathological conditions, including the neurodegenerative disorders, all cancers, and diabetes. In Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors, expert laboratorians detail emerging methodologies for studying the cell cycle, its kinases, and kinase inhibitors. The authors focus on issues of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Many of the protocols are new, such as electron microsocopy to detect apoptosis and proteosome-mediated degradation, whereas others study the interactions between protein-protein, protein-DNA, and protein-RNA. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. State-of-the-art and highly practical, Cell Cycle Control and Dysregulation Protocols: Cyclins, Cyclin-Dependent Kinases, and Other Factors offers pathbreaking researchers powerful tools for probing the cell cycle regulation across a wide variety of pathologies Bibliography Includes bibliographical references and index Subject Cell cycle -- Laboratory manuals Cyclin-dependent kinases -- Laboratory manuals Cyclins -- Laboratory manuals Biology. Immunohistochemistry. Immunocytochemistry. Nucleic acids. Proteins. Immunodiagnosis. Life sciences. Protein kinases. Physical sciences. Histology -- Technique. Diagnosis. Medical laboratory technology. Phosphotransferases. Transferases. Enzymes. DNA. Gene expression. Fluorescent antibody technique. Cyclin-dependent kinases. Biology Immunohistochemistry Nucleic Acids Protein Serine-Threonine Kinases Genetic Phenomena Proline-Directed Protein Kinases Proteins Peptides Immunologic Tests Amino Acids, Peptides, and Proteins Biological Science Disciplines Protein Kinases Histocytochemistry Nucleic Acids, Nucleotides, and Nucleosides Chemicals and Drugs Natural Science Disciplines Immunologic Techniques Phenomena and Processes Histological Techniques Laboratory Techniques and Procedures Phosphotransferases (Alcohol Group Acceptor) Diagnosis Clinical Laboratory Techniques Phosphotransferases Investigative Techniques Disciplines and Occupations Analytical, Diagnostic and Therapeutic Techniques and Equipment Transferases Enzymes Enzymes and Coenzymes Cell Cycle Proteins DNA Gene Expression Fluorescent Antibody Technique Cyclin-Dependent Kinases Intracellular Signaling Peptides and Proteins Genetics biology. protein. biological sciences. physical sciences. diagnosis. enzyme. SCIENCE -- Life Sciences -- Developmental Biology. Transferases Proteins Protein kinases Physical sciences Phosphotransferases Nucleic acids Medical laboratory technology Life sciences Immunohistochemistry Immunodiagnosis Immunocytochemistry Histology -- Technique Gene expression Fluorescent antibody technique Enzymes DNA Diagnosis Biology Cell cycle Cyclin-dependent kinases Cyclins Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Giordano, Antonio, MD. Romano, Gaetano, 1959- LC no. 2004006931 ISBN 0896039498 9780896039490 1592598226 9781592598229

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