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Title Immunocytochemical methods and protocols / edited by Lorette C. Javois
Published Totowa, N.J. : Humana Press, ©1994


Description 1 online resource (xii, 435 pages) : illustrations
Series Methods in molecular biology ; v. 34
Methods in molecular biology (Clifton, N.J.) ; v. 34. 1064-3745
Contents Antibody preparation -- Overview of antibody use in immunocytochemistry -- Purification of antibodies using ammonium sulfate fractionation or gel filtration -- Purification of antibodies using ion-exchange chromatography -- Purification of antibodies using affinity chromatography -- Purification of antibodies using protein A-sepharose and FPLC -- Conjugation of fluorochromes to antibodies -- Biotinylation of antibodies -- Tissue preparation for light microscopic analyses -- Overview of cell fixation and permeabilization -- Preparation of frozen sections for analysis -- Processing of cell-touch preparations -- Processing of cytological specimens -- Processing of tissue-culture cells -- Processing of tissue specimens -- Light microscopic detection systems -- Overview of fluorophores -- Direct immunofluorescent labeling of cells -- Fluorescence labeling of surface antigens of attached or suspended tissue-culture cells -- Fluorescence labeling of intracellular antigens of attached or suspended tissue-culture cells -- Fluorescent labeling of surface or intracellular antigens in whole-amounts -- Overview of antigen detection through enzymatic activity -- Peroxidase-antiperoxidase (PAP) method -- Avidin-biotin complex (ABC) method -- Labeled avidin binding (LAB) method -- Avidin-biotin labeling of cellular antigens in cryostat-sectioned tissue -- ELISA on attachment-dependent or suspension grown cells -- Use of immunogold with silver enhancement -- Fluorescence-activated cell sorter (FACS) analyses -- Overview of flow cytometry and fluorescent probes for cytometry -- Tissue disaggregation -- Indirect immunofluorescent labeling of viable cells -- Indirect immunofluorescent labeling of fixed cells -- Fluorescent labeling of DNA -- Deparaffinization and processing of pathologic material -- Assay for phagocytosis -- Assay for filamentous actin -- Assay for chemoattractant binding -- Assay for oxidative metabolism -- Colloidal gold detection systems for electron microscopic analyses -- Fixation and embedding -- Preparation of colloidal gold -- Conjugation of colloidal gold to proteins -- Colloidal gold/streptavidin methods -- Pre-embedding labeling methods -- Postembedding labeling methods -- Photography -- Overview of fluorescence photomicrography -- Special applications -- Overview of fluorescence analysis with the confocal microscope -- Overview of laser microbeam applications as related to antibody targeting -- Nucleic acid immunocytochemistry : in situ hybridization to human chromosomes of an alkaline phosphatase-labeled centromeric probe -- Fluorescence in situ hybridization using whole chromosome library probes -- Overview of automated immunostainers -- Overview of immunocytochemical approaches to the differential diagnosis of tumors: diagnostic applications in the clinical laboratory
Summary The principle that antibodies can be used as cytochemical agents provided they are tagged with suitable markers has been evident for over 50 years. During this time the use of immunocytochemical methods has spread to a wide array of biological disciplines. Early applications focused on the detection of microbial antigens in tissues, while more recent applications have used monoclonal antibodies to study cell differentiation during embryonic development. For a select few disciplines, volumes have been published focusing on the specific application of immunocytochemical techniques to that discipline. What distinguishes the present book, Immunocytochemical Methods and Protocols, from earlier books is its broad appeal to researchers in all disciplines, including those in both research and clinical settings. The methods and protocols presented here are designed to be general in their application and the accompanying "Notes" provide invaluable assistance in adapting or troubleshooting the protocols. Interspersed throughout the book are chapters providing overviews of select topics related to immunocytochemistry
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Immunocytochemistry -- Methodology
Immunohistochemistry -- standards
Immunohistochemistry -- methods
Immunocytochemistry -- Methodology
Genre/Form Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Javois, Lorette C.
ISBN 9781592595211