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E-book

Title Overview of blotting techniques
Published Ashland : Delve Publishing, 2020
Online access available from:
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Description 1 online resource (255 p.)
Contents Cover -- Copyright -- DECLARATION -- ABOUT THE EDITORS -- TABLE OF CONTENTS -- List of Contributors -- List of Abbreviations -- Preface -- Chapter 1 Western Blotting-the Works -- 1.1 Concept -- 1.2 The Details -- 1.3 General Protocol For Western Blotting -- 1.4 Troubleshooting -- 1.5 Advances In Western Blot -- 1.6 Final Word -- 1.7 References -- Chapter 2 Southern Blotting and Associated Techniques -- 2.1 Introduction -- 2.2 Interesting Insights: The Role of Dr. Southern In Science -- 2.3 Technology Types -- 2.4 The Southern Blotting Principle -- 2.5 Recent Alterations To Southern Blotting
2.6 Dna Pre-Preparation Before Southern Blotting -- 2.7 Techniques For Dna Blotting -- 2.8 The Southern Blot Prehybridization -- 2.9 Washing Steps and Hybridization -- 2.10 Role of Southern Blotting In Identification of Clones -- 2.11 Polymorphism Analysis of Restriction Fragment Length -- 2.12 Final Words -- 2.13 References -- Chapter 3 Northern Blotting: A Brief Overview -- 3.1 Introduction -- 3.2 The Procedure In Brief -- 3.3 Applications Of The Northern Blotting Technique -- 3.4 Drawbacks Of The Northern Blotting Technique (Taniguchi Et Al., 2001 -- Karen Reue, 1998)
3.5 The Reverse Northern Blot Technique -- 3.6 The Northwestern Blot -- 3.7 Summary -- 3.8 References -- Chapter 4 Eastern Blotting -- 4.1 Outline -- 4.2 Case Study 1: Eastern Blotting For A Herb Vs. Tlc (Thin Layer Chromatography) -- 4.3 Case Study 2: Eastern Blotting For Bioactive Compounds -- 4.4 References -- Chapter 5 Misleading Westerns: Common Quantification Mistakes In Western Blot Densitometry And Proposed Corrective Measures -- Abstract -- Introduction -- Materials And Methods -- Results -- Discussion -- Conclusions -- Data Availability -- Conflicts Of Interest -- Acknowledgments
Careful Experimental Design Produces Reliable And Reproducible Data -- Sample Preparation -- Determine The Linear Dynamic Range of Protein Loading -- Determine The Appropriate Reference Signal For Data Normalization -- Data Analysis: The Background Subtraction Problem -- Computational Analysis of Densitometric Data -- Conclusions -- Abbreviations -- Conflict Of Interests -- References -- Chapter 9 Characterization Of Nora Virus Structural Proteins Via Western Blot Analysis -- Abstract -- Introduction -- Materials And Methods -- Discussion -- Conclusion -- Competing Interests
Notes Authors' Contributions
Description based upon print version of record
Bibliography References -- Chapter 6 Application of Monoclonal Antibodies Against Bioactive Natural Products: Eastern Blotting And Preparation of Knockout Extract -- Abstract -- Introduction -- Preparation of Mab Against Natural Products -- Conclusions -- References -- Chapter 7 Evaluating Strategies To Normalise Biological Replicates of Western Blot Data -- Abstract -- Introduction -- Materials And Methods -- Results -- Discussion -- Supporting Information -- Acknowledgments -- Funding Statement -- References -- Chapter 8 The Design of A Quantitative Western Blot Experiment -- Abstract -- Introduction
Subject Immunoblotting.
Macromolecules.
Western immunoblotting.
Immunoblotting.
Macromolecules.
Western immunoblotting.
Form Electronic book
Author Pandey, Prerna.
Sripathi, Shiv Sanjeevi
ISBN 1774078945
9781774078945