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Title Antibody-drug conjugates / edited by Laurent Ducry
Published New York : Humana Press, 2013

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Description 1 online resource (x, 317 pages) : illustrations
Series Methods in Molecular Biology, 1064-3745 ; 1045
Methods in molecular biology (Clifton, N.J.) ; v. 1045. 1064-3745
Contents Antibody-Drug Conjugate (ADC) Clinical Pipeline: A Review -- Antibody-Drug Conjugate Target Selection: Critical Factors -- Selecting an Optimal Antibody for Antibody-Drug Conjugate Therapy: Internalization and Intracellular Localization -- Antibody-Drug Conjugate Payloads -- Linker Technologies for Antibody-Drug Conjugates -- In Vivo Testing of Drug-Linker Stability -- Pharmacokinetics and ADME Characterizations of Antibody-Drug Conjugates -- Safe Handling of Cytotoxic Compounds in a Biopharmaceutical Environment -- Micro- and Mid-Scale Maleimide-Based Conjugation of Cytotoxic Drugs to Antibody Hinge Region Thiols for Tumor Targeting -- Protocols for Lysine Conjugation -- Engineering THIOMABs for Site-Specific Conjugation of Thiol-Reactive Linkers -- Enzymatic Antibody Modification by Bacterial Transglutaminase -- Formulation Development of Antibody-Drug Conjugates -- Conjugation Process Development and Scale-Up -- Methods for Conjugating Antibodies to Nanocarriers -- Drug-to-Antibody Ratio (DAR) by UV/Vis Spectroscopy -- Drug-to-Antibody Ratio (DAR) and Drug Load Distribution by Hydrophobic Interaction Chromatography and Reversed Phase High Performance Liquid Chromatography -- Drug-to-Antibody Ratio (DAR) and Drug Load Distribution by LC-ESI-MS -- Determination of Charge Heterogeneity and Level of Unconjugated Antibody by Imaged cIEF -- Risk-Based Scientific Approach for Determination of Extractables/Leachables from Biomanufacturing of Antibody-Drug Conjugates (ADCs)
Summary Antibody-drug conjugates (ADCs) represent a promising therapeutic approach for cancer patients by combining the antigen-targeting specificity of monoclonal antibodies (mAbs) with the cytotoxic potency of chemotherapeutic drugs. In Antibody-Drug Conjugates, expert researchers provide detailed protocols for many of the key ADC techniques necessary for working in the field. These chapters and methodologies are aimed at the key tasks necessary to identify a suitable target, properly design the mAb, the linker and the payload, as well as to conjugate them in a reproducible and scalable fashion. Written in the highly successful Methods in Molecular Biology format, these detailed chapters include the kind of practical implementation advice that guarantees quality results. Authoritative and timely, Antibody-Drug Conjugates aims to further drive ADC development and thus help toward improving cancer treatments of the future
Analysis farmacologie
pharmacology
toxicologie
toxicology
biomedische wetenschappen
biomedicine
oncologie
oncology
Medicine (General)
Geneeskunde (algemeen)
Notes Includes index
Bibliography Includes bibliographical references and index
Notes English
Online resource; title from PDF title page (SpringerLink, viewed August 20, 2013)
Subject Antibody-drug conjugates -- Laboratory manuals
Antibody-toxin conjugates -- Laboratory manuals
Immunoglobulins.
Diseases.
Blood proteins.
Globulins.
Proteins.
Monoclonal antibodies.
Antibody-toxin conjugates.
Drug interactions.
Drugs.
Immunoglobulins
Disease
Chemicals and Drugs
Therapeutic Uses
Immunoproteins
Pharmacologic Actions
Chemical Actions and Uses
Serum Globulins
Blood Proteins
Globulins
Proteins
Amino Acids, Peptides, and Proteins
Neoplasms
Antibodies, Monoclonal
Antineoplastic Agents
Antibodies
Immunoconjugates
Drug Combinations
Pharmaceutical Preparations
protein.
Proteins
Monoclonal antibodies
Immunoglobulins
Globulins
Drugs
Drug interactions
Diseases
Blood proteins
Antibody-drug conjugates
Antibody-toxin conjugates
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Ducry, Laurent, editor.
ISBN 9781627035415
1627035419
1627035400
9781627035408
1927035414
9781927035412