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Book Cover
E-book

Title Handbook of ELISPOT : methods and protocols / edited by Alexander E. Kalyuzhny
Edition 2nd ed
Published Totowa, N.J. : Humana Press, ©2012

Copies

Description 1 online resource (x, 261 pages) : illustrations
Series Springer protocols
Methods in molecular biology, 1940-6029 ; v. 792
Springer protocols (Series)
Methods in molecular biology (Clifton, N.J.) ; volume 792. 1064-3745
Contents Unique strengths of ELISPOT for T cell diagnostics -- The impact of harmonization on ELISPOT assay performance -- Equine ELISPOT assay to study secretion of IFN[gamma] and IL-4 from peripheral blood mononuclear cells -- Utilization of feline ELISPOT for mapping vaccine epitopes -- Analyzing cellular immunity to AAV in a canine model using ELISPOT assay -- Dual- and triple-color fluorospot -- ELISPOT assay as a tool to study oxidative stress in lymphocytes -- ELISPOT assay for neuroscience research: Studying TNT[alpha] secretion from microglial cells -- ELISPOT assay as a tool to study the effects of stem cells on cytokine secretion -- Combining ELISPOT and ELISA to measure amounts of cytokines secreted by a single cell -- How ELISPOT morphology reflects on the productivity and kinetics of cells' secretory activity -- Mathematical algorithms for automatic search, recognition, and detection of spots in ELISPOT assay -- Objective, user-independent ELISPOT data analysis based on scientifically validated principles -- Statistical analysis of ELISPOT assays -- Response determination criteria for ELISPOT: Toward a standard that can be applied across laboratories -- Detection of vaccinia virus-specific IFN[gamma] and IL-10 secretion from human pbmcs and CD8(+) T cells by ELISPOT -- ELISPOT assays to enumerate bovine IFN-[gamma]-secreting cells for the development of novel vaccines against bovine tuberculosis -- Il-7 addition increases spot size and number as measured by T-SPOT. TB -- Overview of membranes and membrane plates used in research and diagnostic ELISPOT assays
Summary Enzyme-linked immunospot assay (ELISPOT) has been known for some time as a unique state-of-the-art technique for studying the cytokine-secreting activity of immune system cells, and it appears to be one of the fast growing applications in biomedical research, becoming an indispensable tool in vaccine development, HIV research, transplantation studies, and cancer and allergy research. The second edition of Handbook of ELISPOT: Methods and Protocols, only the second book in the field which is entirely dedicated to ELISPOT assay, shares the detailed techniques that have been developed since the release of the popular first edition. Straight from the labs of seasoned experts, this book covers setting and performing ELISPOT assays, ELISPOT for veterinary research, advanced ELISPOT techniques, image and data analysis, as well as vaccine development and diagnostics. Written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Handbook of ELISPOT: Methods and Protocols, Second Edition serves as a compilation of a technical reference and a troubleshooting guide for researchers, both experienced and novice, worldwide in order to advance the usage of this key tool
Analysis biomedische wetenschappen
biomedicine
geneeskunde
medicine
immunologie
immunology
Medicine (General)
Geneeskunde (algemeen)
Bibliography Includes bibliographical references and index
Notes English
Online resource; title from PDF title page (Springer, viewed October 5, 2011)
Subject Enzyme-linked immunosorbent assay.
Lymphocytes.
Cytology -- Technique.
Medical laboratory technology.
Biologicals.
Biological products.
Proteins.
Immunity.
Immunoassay.
Immune system.
Immunohistochemistry.
Immunocytochemistry.
Leucocytes.
Blood cells.
Immunodiagnosis.
Cells.
Blood.
Anatomy.
Diagnosis.
Cytokines.
Methodology.
T cells.
Cellular immunity.
Enzyme-Linked Immunosorbent Assay
Intercellular Signaling Peptides and Proteins
Adaptive Immunity
Lymphocytes
Investigative Techniques
Publication Formats
Cytological Techniques
Analytical, Diagnostic and Therapeutic Techniques and Equipment
Clinical Laboratory Techniques
Immunoenzyme Techniques
Publication Characteristics
Immunosorbent Techniques
Biological Factors
Peptides
Proteins
Leukocytes, Mononuclear
Immunity
Immunoassay
Amino Acids, Peptides, and Proteins
Immune System Phenomena
Immunohistochemistry
Leukocytes
Chemicals and Drugs
Blood Cells
Immunologic Tests
Molecular Probe Techniques
Immune System
Phenomena and Processes
Immunologic Techniques
Cells
Hemic and Immune Systems
Laboratory Techniques and Procedures
Blood
Anatomy
Diagnosis
Cytokines
Enzyme-Linked Immunospot Assay
Methods
Laboratory Manuals
T-Lymphocytes
Immunity, Cellular
Animal Structures
Thymocytes
Biological Products
protein.
blood (animal material)
anatomy.
diagnosis.
methodology.
enzyme-linked immunosorbent assay.
T cells
Proteins
Methodology
Medical laboratory technology
Lymphocytes
Leucocytes
Immunohistochemistry
Immunodiagnosis
Immunocytochemistry
Immunoassay
Immunity
Immune system
Diagnosis
Cytology -- Technique
Cytokines
Cellular immunity
Cells
Blood cells
Blood
Biologicals
Biological products
Anatomy
Enzyme-linked immunosorbent assay
Form Electronic book
Author Kalyuzhny, Alexander E
ISBN 9781617793257
1617793256