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Book Cover

Title Immunochemical protocols / edited by Robert Burns
Edition 3rd ed
Published Totowa, N.J. : Humana Press, ©2005


Description 1 online resource (viii, 317 pages) : illustrations
Series Methods in molecular biology ; 295
Methods in molecular biology (Clifton, N.J.) ; v. 295
Contents Immunization strategies for antibody production / Robert Burns -- Synthetic peptides as antigens for antibody production / David C. Hancock and Nicola J. O'Reilly -- Production of polyclonal antibody in rabbits / David C. Hancock and Nicola J. O'Reilly -- Making hybridomas / Robert Burns -- Growing hybridomas / Gary Entrican and Gareth Young -- Phage display vectors for the in vitro generation of human antibody fragments / Michael Hust and Stefan Dobel -- Purification of antibodies and preparation of antibodies fragments / Graham D. Kemp -- Conjugation of antibodies to alkaline phosphatase / G. Brian Wisdom -- Conjugation of antibodies to horseradish peroxidase / G. Brian Wisdom -- Conjugation of antibodies to fluorescein or rhodamine / G. Brian Wisdom -- Conjugation of antibodies to biotin / James D. Hirsch and Rosaria P. Haughland -- Preparation of colloidal gold probes / David Hughes -- Immunogold probes in light microscopy / David Hughes -- Immunoelectron microscope techniques in plant virus diagnosis / Daphne M. Wright -- Dual-antibody sandwich enzyme-linked immunosorbent assay / William Jordan -- Competitive enzyme-linked immunosorbent assay / William Jordan -- Immunoblotting techniques / Barbara Magi and Sabrina Liberatori -- Epitope mapping / Glenn E. Morris -- Generation and testing of immunoaffinity columns / Weilin L. Shelver -- Immunocapture-polymerase chain reaction / Vincent Mulholland -- Magnetic cell sorting / Maciej Zborowski and Jeffrey J. Chalmers -- Cell enrichment and immunochemical staining / Oscar R. Lara and Jeffrey J. Chalmers
Summary This new third edition of a much praised classic has been updated and revised to provide both basic methods for producing and using antibodies and advanced techniques required by today's state-of-the art investigators. The basic sections of the book stress the first principles of antibody production and guide the reader through the processes and pitfalls of immunochemistry. The advanced sections focus on specific applications and cover the major immunochemical techniques currently used in laboratories. New material includes techniques for making new monoclonal cell lines, for producing good quality monoclonal antibodies, immunocapture PCR, immunoaffinity purification, and immunoelectron microscopy in plant virus diagnosis. The authors provide full details of methods for antigen selection and preparation, antibody production, and reagent manufacture. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Up-to-date and highly practical, Immunochemical Protocols, Third Edition, takes both novice and experienced researchers from project initiation to final test format with a broad array of protocols suited to immediate use in the laboratory, as well as methods that can be successfully applied to entirely new systems and processes
Bibliography Includes bibliographical references and index
Notes English
Print version record
Subject Immunochemistry -- Laboratory manuals
Antibodies -- isolation & purification
Immunochemistry -- methods
MEDICAL -- Immunology.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Burns, Robert, 1959-
LC no. 2004009405
ISBN 1588292746