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Title Epstein-Barr virus : methods and protocols / edited by Janos Minarovits, Hans Helmut Niller
Published New York : Humana Press : Springer, ©2017


Description 1 online resource (xiv, 269 pages) : color illustrations
Series Methods in molecular biology ; 1532
Methods in molecular biology (Clifton, N.J.) ; v. 1532
Contents Current trends and alternative scenarios in EBV research / Janos Minarovits and Hans Helmut Niller -- Epstein-Barr virus : clinical ciagnostics / Hans-Helmut Niller and Georg Bauer -- Establishment of EBV-infected lymphoblastoid cell lines / Noemi Nagy -- Generation and infection of organotypic cultures with Epstein-Barr virus / Rachel M. Temple, Craig Meyers, and Clare E. Sample -- Affinity purification-mass spectroscopy methods for identifying Epstein-Barr virus-host interactions / Anna A. Georges and Lori Frappier -- Use of 3D telomere FISH for the characterization of the nuclear architecture in EBV-positive hodgkin's lymphoma / Hans Knecht and Sabine Mai -- Analysis of EBV transcription using high-throughput RNA sequencing / Tina O'Grady, Melody Baddoo, and Erik K. Flemington -- Analysis of viral promoter usage in EBV-infected cell lines : a comparison of qPCR following conventional RNA isolation and nuclear run-on assay / Kalman Szenthe and Ferenc Banati -- Analysis of viral and cellular microRNAs in EBV-infected cells / Rebecca L. Skalsky -- Isolation and characterization of exosomes released by EBV-immortalized cells / Gulfaraz Khan and Waqar Ahmed -- Functional analysis of exosomes derived from EBV-infected cells / Gulfaraz Khan and Pretty S. Philip -- Terminal repeat analysis of EBV genomes / Ferenc Bánáti, Anita Koroknai, and Kálmán Szenthe -- Characterization of EBV promoters and coding regions by sequencing PCR-amplified DNA fragments / Kalman Szenthe and Ferenc Bánáti -- Use of chromatin precipitation coupled to DNA sequencing (ChIP-Seq) for the analysis of Zta binding to the human and EBV genome / Anja Godfrey, Sharada Ramasubramanyan, and Alison J. Sinclair -- Analysis of viral epigenotypes using bisulfite sequencing : a detailed protocol for the crucial bisulfite modification and PCR amplification steps / Daniel Salamon -- Analysis of viral epigenotypes using chromatin immunoprecipitation / Ferenc Bánáti and Kálmán Szenthe -- Mice with reconstituted human immune system components as a tool to study immune cell interactions in EBV infection / Frank Heuts and Noemi Nagy -- Generation and analysis of humanized mouse model of EBV infection / Ken-Ichi Imadome and Shigeyoshi Fujiwara -- EBV-directed T cell therapeutics for EBV-associated lymphomas / Lauren P. McLaughlin, Stephen Gottschalk, Cliona M. Rooney, and Catherine M. Bollard
Summary This volume explores data from the applications of molecular biological methods and the applications of recent immunological and cytogenetic methods in Epstein-Barr Virus (EBV) that will offer readers possible new solutions to the unresolved problems in the EBV field. Chapters in this book cover topics such as: viral life cycle, latency, EBV-associated diseases and EBV diagnostics; in vitro methods including organotypic cultures for the analysis of EBV-epithelial cell interactions; identification of the interacting viral and cellular proteins using affinity purification-mass spectrometry methods; 3D telomere FISH; transcription analysis using high-throughput RNA sequencing, qPCR and nuclear run-on assay; analysis of viral and cellular microRNAs; isolation and characterization of exosomes and the assessment of their function; characterization of the viral genome by terminal repeat analysis and sequencing; the use of chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for the analysis of Zta-DNA interactions; epigenetic analysis by bisulfite sequencing and ChIP; novel in vivo models for the study of EBV infection; and how immunological, virological, tissue culture and molecular methods can be combined to yield Good Manufacturing Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative diseases (PTLD). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Epstein Barr-Virus: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerProtocols, viewed December 6, 2016)
Subject Epstein-Barr virus -- Laboratory manuals
Human beings.
Herpesvirus 4, Human -- physiology
Herpesvirus 4, Human -- isolation & purification
Immunoassay -- methods
Viral Proteins -- chemistry
Viral Proteins -- genetics
Virus Cultivation -- methods
Homo sapiens (species)
Epstein-Barr virus
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Minarovits, Janos, editor
Niller, Hans Helmut, editor
ISBN 9781493966554