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Title Epigenetics and gene expression in cancer, inflammatory and immune diseases / edited by Barbara Stefanska, David J. MacEwan
Published New York, NY : Humana Press, 2017

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Description 1 online resource (xii, 197 pages) : illustrations (some color)
Series Methods in pharmacology and toxicology, 1557-2153
Methods in pharmacology and toxicology, 1557-2153
Contents High-Throughput Techniques for DNA Methylation Profiling -- Reduced Representation Bisulfite Sequencing (RRBS) and Cell Sorting Prior to DNA Methylation Analysis in Psychiatric Disorders -- Targeted DNA Methylation Analysis Methods -- Analyzing Targeted Nucleosome Position and Occupancy in Cancer, Obesity, and Diabetes -- Synthesis and Application of Cell-Permeable Metabolites for Modulating Chromatin Modifications Regulated by a-Ketoglutarate-Dependent Enzymes -- High-Throughput Screening of Small Molecule Transcriptional Regulators in Embryonic Stem Cells Using qRT-PCR -- Methods for MicroRNA Profiling in Cancer -- Microbiota and Epigenetic Regulation of Inflammatory Mediators -- Optical Microscopy and Spectroscopy for Epigenetic Modifications in Single Living Cells -- MicroRNAs in Therapy and Toxicity -- Genetics and Epigenetics of Multiple Sclerosis
Summary This practical collection examines methodologies originating from the benefits of genome-wide approaches to studying epigenetics, which has opened the emerging field of epigenomics. Focusing on the areas of cancer, inflammatory and autoimmune disorders, chapters discuss three main components of the epigenome and their role in the regulation of gene expression and present a detailed method section specific to studying each component, including data analyses, troubleshooting, and feasibility in different experimental settings. The main topics are high-throughput and targeted methods for DNA methylation analysis, nucleosome position mapping, studying epigenetic effects of gut microbiota, optical imaging for detection of epigenetic aberrations in living cells, methods for microRNA, and histone code profiling. Written for the Methods in Pharmacology and Toxicologyseries, the book includes the kind of detail and implementation advice to encourage success in the lab. Authoritative and easily applicable, Epigenetics and Gene Expression in Cancer, Inflammatory and Immune Diseases aims to provide pharmacologists, molecular biologists, bioinformaticians, and toxicologists with a vital background on epigenetics and state-of-the-art techniques in epigenomics
Notes Includes index
Bibliography Includes bibliographical references and index
Notes Online resource; title from PDF title page (SpringerLink, viewed December 21, 2016)
Subject Epigenetics -- Laboratory manuals
Gene expression -- Laboratory manuals
Epigenomics -- methods
Gene Expression -- genetics
Neoplasms -- genetics
Inflammation -- genetics
Immune System Diseases -- genetics
Pharmacology.
Medical -- Pharmacology.
Epigenetics
Gene expression
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Stefanska, Barbara, editor.
MacEwan, David J., editor
ISBN 9781493967438
1493967436
149396741X
9781493967414