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Title Human cell culture protocols / edited by Gareth E. Jones
Published Totowa, N.J. : Humana Press, [1996]
©1996

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Location Call no. Vol. Availability
 MELB  611.0181 Jon/Hcc  AVAILABLE
Description xiv, 545 pages : illustrations ; 24 cm
Series Methods in molecular biology
Methods in molecular biology (Clifton, N.J.)
Contents 1. Establishment and Maintenance of Normal Human Keratinocyte Cultures / Claire Linge -- 2. Cultivation of Normal Human Epidermal Melanocytes / Mei-Yu Hsu and Meenhard Herlyn -- 3. Cultivation of Keratinocytes from the Outer Root Sheath of Human Hair Follicles / Alain Limat and Thomas Hunziker -- 4. Isolation and Short-Term Culture of Human Epidermal Langerhans' Cells / Corinne Moulon -- 5. Growth and Differentiation of Human Adipose Stromal Cells in Culture / Brenda Strutt, Wahid Khalil and Donald Killinger -- 6. Human Fetal Brain Cell Culture / Mark P. Mattson -- 7. Culture of Human Normal Brain and Malignant Brain Tumors for Cellular, Molecular, and Pharmacological Studies / Francis Ali-Osman -- 8. Cell Culture of Human Brain Tumors on Extracellular Matrices: Methodology and Biological Applications / Manfred Westphal, Hildegard Nausch and Dorothea Zirkel -- 9. Isolation and Culture of Human Umbilical Vein Endothelial Cells / David M. L. Morgan -- 10. Human Thymic Epithelial Cell Cultures / Anne H. M. Galy -- 11. Generation and Cloning of Antigen-Specific Human T-Cells / Hans Yssel -- 12. Protection of Purified Human Hematopoietic Progenitor Cells by Interleukin-1[beta] or Tumor Necrosis Factor-[alpha] / Robert J. Colinas -- 13. Human Mononuclear Phagocytes in Tissue Culture / Yona Keisari -- 14. Purification of Peripheral Blood Natural Killer Cells / Ian M. Bennett and Bice Perussia -- 15. Cystic Fibrosis Airway Epithelial Cell Culture / Manuel A. Vega -- 16. Gene Transfer into Cystic Fibrosis Airway Epithelial Cells / Manuel A. Vega -- 17. Genetic Analysis of Cystic Fibrosis Airway Epithelial Cells / Manuel A. Vega -- 18. Human Tracheal Gland Cells in Primary Culture / Marc D. Merten -- 19. Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation / Mary B. Goldring -- 20. Isolation and Culture of Bone-Forming Cells (Osteoblasts) from Human Bone / James A. Gallagher, Roger Gundle and Jon N. Beresford -- 21. The Isolation of Osteoclasts from Human Giant Cell Tumors and Long-Term Marrow Cultures / Catherine A. Walsh, John A. Carron and James A. Gallagher -- 22. In Vitro Cellular Systems for Studying OC Function and Differentiation: Primary OC Cultures and the FLG 29.1 Model / Donatella Aldinucci, Julian M. W. Quinn, Massimo Degan, Senka Juzbasic, Angela De Iuliis, Salvatore Improta, Antonio Pinto and Valter Gattei -- 23. Isolation, Purification, and Growth of Human Skeletal Muscle Cells / Grace K. Pavlath -- 24. Cultures of Proliferating Vascular Smooth Muscle Cells from Adult Human Aorta / Heide L. Kirschenlohr, James C. Metcalfe and David J. Grainger -- 25. Human Myometrial Smooth Muscle Cells and Cervical Fibroblasts in Culture: A Comparative Study / Francoise Cavaille, Dominique Cabrol and Francoise Ferre -- 26. Primary Culture of Human Antral Endocrine Cells / Alison M. J. Buchan -- 27. Primary Cell Culture of Human Enteric Neurons: Submucosal Plexus / Eric A. Accili and Alison M. J. Buchan -- 28. Isolation and Culture of Human Hepatocytes / Martin K. Bayliss and Paul Skett -- 29. Culture of Human Pancreatic Islet Cells / Stellan Sandler and Decio L. Eizirik -- 30. Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules / Vicente Rodilla and Gabrielle M. Hawksworth -- 31. Glomerular Epithelial and Mesangial Cell Culture and Characterization / Heather M. Wilson, Keith N. Stewart and Alison M. MacLeod -- 32. Enzymatic Isolation and Serum-Free Culture of Human Renal Cells: Retaining Properties of Proximal Tubule Cells / John H. Todd, Kenneth E. McMartin and Donald A. Sens -- 33. Culture of Human Renal Medullary Interstitial Cells / Klara Tisocki and Gabrielle M. Hawksworth -- 34. Culture Technique of Human Pituitary Adenoma Cells / Masanori Kabuto -- 35. Preparation of Human Trophoblast Cells for Culture / Suren R. Sooranna -- 36. Superfused Microcarrier Cultures of BeWo Choriocarcinoma Cells: A Dynamic Model for Studies of Human Trophoblast Function / Suren R. Sooranna and Bryan M. Eaton -- 37. Corneal Organ Culture / Robert W. Lambert, Normand R. Richard and Janet A. Anderson -- 38. Keratocyte Cell Culture / Normand R. Richard, Robert W. Lambert and Janet A. Anderson -- 39. Conjunctiva: Organ and Cell Culture / Monica Berry, Roger B. Ellingham and Anthony P. Corfield -- 40. Establishment and Maintenance of In Vitro Cultures of Human Retinal Pigment Epithelium / Eva L. Feldman, Monte A. Del Monte, Martin J. Stevens and Douglas A. Greene -- 41. Demonstration of Mycoplasma Contamination in Cell Cultures by a Mycoplasma Group-Specific Polymerase Chain Reaction / Frank van Kuppeveld, Jos van der Logt, Karl-Erik Johansson and Willem Melchers
Summary "In Human Cell Culture Protocols expert scientific and clinical investigators present proven cell culture techniques applicable to tissue samples taken from a wide variety of human organs, particularly those prone to pathological change. They describe in clear, step-by-step instructions the special requirements for successfully culturing such human cells as T-cells, trophoblast cells, renal cells, natural killer cells, endothelial cells, neurons, epithelial cells, pituitary cells, and more."--BOOK JACKET. "Human Cell Culture Protocols eliminates much of the chore of adapting techniques initially developed for animal cell culture systems, and much of the time consumed in hunting down potentially useful techniques buried in the details of research papers, or even in books largely devoted to animal cell culture. Human Cell Culture Protocols ensures that investigators - whether novice or skilled - can use quickly and successfully high quality, in vitro cultures as models to explore disease processes."--BOOK JACKET
Notes Includes index
Bibliography Includes bibliographical references
Notes Also available online via the World Wide Web, by subscription to BioMedProtocols.com (BMP) via Humana Press
Subject Human cell culture -- Laboratory manuals.
Cells, Cultured.
Cytological Techniques.
Author Jones, Gareth.
Humana Press.
LC no. 96004625
ISBN 089603335X