| Description |
1 online resource (xiv, 415 pages) : illustrations (some color) |
| Series |
Methods in molecular biology, 1940-6029 ; volume 1510 |
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Springer protocols |
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Methods in molecular biology (Clifton, N.J.) ; v. 1510. 1064-3745
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Springer protocols (Series)
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| Contents |
Detection of autophagy induction after HDAC inhibitor treatment in leukemic cells / Anja Göder, Nisintha Mahendrarajah, and Oliver H. Krämer -- Assessment of HDACi-induced protein cleavage by caspases / Fabian Treude [and others] -- Assessment of HDACi-induced cytotoxicity / Lisa Marx-Blümel [and others] -- Analysis of HDACi-induced changes in chromosomal passenger complex localization / Britta Unruhe-Knauf and Shirley K. Knauer -- Analysis of histone deacetylase-dependent effects on cell migration using the stripe assay / Sonja Mertsch and Solon Thanos -- Using histone deacetylase inhibitors to analyze the relevance of HDACs for translation / Darren M. Hutt [and others] -- Effects of HDACi on immunological functions / René Winkler and Christian Kosan -- DNA fiber spreading assay to test HDACi effects on DNA and its replication / Teodora Nikolova [and others] -- Assessing the effect of class I histone deacetylase activity on DNA double-strand break repair by homologous recombination / Andrea Krumm and Wynand P. Roos -- Establishment and characterization of long-term cultures derived from primary acute myeloid leukemia cells for HDAC inhibitor research / Annette Romanski and Gesine Bug -- Assessing the histone deacetylase activity of SIRT6 in primary murine hepatocytes via proximity ligation assay / Katharina Wolf and Susanne Strand -- Purification and analysis of male germ cells from Adult Mouse Testis / Thierry Buchou [and others] -- Generation of tissue-specific mouse models to analyze HDAC functions / Astrid Hagelkruys, Mirjam A. Moser, and Christian Seiser -- Engineering of conditional class I Hdac knockout mice and generation of a time-spatial knockout by a dual recombination system / Sieglinde Bayer and Matthias Wirth -- Xenografting of cancer cell lines for in vivo screening of the therapeutic potential of HDAC inhibitors / Daniel Nettersheim, Sina Jostes, and Hubert Schorle -- Generation of xenotransplants from human cancer biopsies to assess anti-cancer activities of HDACi / Christina Susanne Mullins [and others] -- Phospho-flow analysis of primary mouse cells after HDAC inhibitor treatment / Dagmar Hildebrand and Katharina F. Kubatzky -- Evaluation of innate immune gene expression following HDAC inhibitor treatment by high throughput qPCR and PhosFlow cytometry / David Olagnier, Cindy Chiang, and John Hiscott -- Assessing HDAC function in the regulation of signal transducer and activator of transcription 5 (STAT5) activity using chromatin immunoprecipitation (ChIP) / Sophia Pinz and Anne Rascle -- Modulation of STAT1-driven transcriptional activity by histone deacetylases / Benjamin Y. Owusu and Lidija Klampfer -- Proximity ligation assay to quantify Foxp3 acetylation in regulatory T cells / Jing Jiao [and others] -- Testing the effects of SIAH ubiquitin E3 ligases on lysine acetyl transferases / Jan Hagenbucher [and others] -- Assessment of HDACi-induced acetylation of nonhistone proteins by mass spectrometry / Martin Wieczorek, Karl-Heinz Gührs, and Thorsten Heinzel -- Assessing the role of paralog-specific sumoylation of HDAC1 / Simona Citro and Susanna Chiocca -- Analysis of histone deacetylases sumoylation by immunoprecipitation techniques / Tobias Wagner, Maren Godmann, and Thorsten Heinzel -- How to distinguish between the activity of HDAC1-3 and HDAC6 with western blot / Mandy Beyer [and others] -- Evaluating the effect of HDAC8 inhibition in malignant peripheral nerve sheath tumors / Gonzalo Lopez and Raphael E. Pollock -- Analyzing the impact of pan- and class-specific HDACi on differentiation-associated factors / Katrin Noack and Oliver H. Krämer -- Encapsulation of the HDACi Ex527 into liposomes and Polymer-Based particles / Dorle Hennig and Diana Imhof -- Preparation of histone deacetylase inhibitor vorinostat-loaded poly D, L-lactide-co-glycolide polymeric nanoparticles by nanoprecipitation method / Vilwanathan Ravikumar and Renu Sankar -- Generation and assessment of fusions between HDACi and TKi / Siavosh Mahboobi, Bernadette Pilsl, and Andreas Sellmer |
| Summary |
This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be used to test experimental hypotheses. Written for the popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step by step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, HDAC HAT Function Assessment and Inhibitor Development: Methods and Protocols serves as an ideal guide to this vital area of study |
| Bibliography |
Includes bibliographical references and index |
| Notes |
Online resource; title from pdf of title page (SpringerProtocols, viewed October 20, 2016) |
| Subject |
Histone deacetylase -- Laboratory manuals
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Genetic regulation.
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Histone Deacetylases -- analysis
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Histone Deacetylase Inhibitors -- analysis
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Histone Acetyltransferases
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Gene Expression Regulation
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Proteins.
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Science -- Life Sciences -- Biochemistry.
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Genetic regulation
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Histone deacetylase
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| Genre/Form |
Laboratory manuals
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Laboratory manuals.
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Manuels de laboratoire.
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| Form |
Electronic book
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| Author |
Kramer, Oliver H., editor
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| ISBN |
9781493965274 |
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1493965271 |
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