Title Sirtuins : methods and protocols / edited by Matthew D. Hirschey, Institute of Molecular Physiology, Duke University Medical Center, Durham, NC, USA

Published New York : Humana Press, 2013

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Description 1 online resource (xi, 314 pages) : illustrations (some color) Series Methods in Molecular Biology, Methods and Protocols, 1064-3745 ; 1077 Methods in molecular biology (Clifton, N.J.) ; v. 1077. 1064-3745 Contents Introduction : sirtuins in aging and diseases / Leonard Guarente -- Sirtuins in yeast : phenotypes and tools / Scott Tsuchiyama [and others] -- C. elegans sirtuins / Mohan Viswanathan and Heidi A. Tissenbaum -- Genetic and biochemical tools for investigating sirtuin function in Drosophila melanogaster / Jason G. Wood, Rachel Whitaker, and Stephen L. Helfand -- Generating mammalian sirtuin tools for protein- interaction analysis / Kathleen A. Hershberger [and others] -- Mass spectrometry-based detection of protein acetylation / Yu Li [and others] -- SILAC-based quantification of Sirt1-responsive lysine acetylome / Yue Chen, Gozde Colak, and Yingming Zhao -- Targeted quantitation of acetylated lysine peptides by selected reaction monitoring mass spectrometry / Matthew J. Rardin, Jason M. Held, and Bradford W. Gibson -- Identification of deacetylase substrates with the biotin switch approach / J. Will Thompson, Alex Robeson, and Joshua L. Andersen -- Assaying chromatin sirtuins / Lei Zhong [and others] -- Measurement of sirtuin enzyme activity using a substrate-agnostic fluorometric nicotinamide assay / Basil P. Hubbard and David A. Sinclair -- Detecting sirtuin-catalyzed deacylation reactions using 32P-labeled NAD and thin-layer chromatography / Anita Zhu, Xiaoyang Su, and Hening Lin -- Chemical acetylation and deacetylation / Kristofer S. Fritz -- Accurate measurement of nicotinamide adenine dinucleotide (NAD+) with high-performance liquid chromatography / Jun Yoshino and Shin-ichiro Imai -- In vivo measurement of the acetylation state of sirtuin substrates as a proxy for sirtuin activity / John Dominy, Pere Puigserver, and Carles Cantó -- Oxygen flux analysis to understand the biological function of sirtuins / Dongning Wang [and others] -- Emerging links between sirtuins and autophagy / In Hye Lee, Jeanho Yun, and Toren Finkel -- Methods to study the role of sirtuins in genome stability / Paloma Martínez-Redondo and Alejandro Vaquero -- Circadian measurements of sirtuin biology / Kathryn Moynihan Ramsey [and others] -- Utilizing calorie restriction to evaluate the role of sirtuins in healthspan and lifespan of mice / Jessica Curtis and Rafael de Cabo Summary Featuring a diverse array of model organisms and scientific techniques, Sirtuins: Methods and Protocols collects detailed contributions from experts in the field addressing this vital family of genes. Opening with methods to generate sirtuin biology tools, the book continues by covering methods to identify sirtuin substrates, to measure sirtuin activity, and to study sirtuin biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Sirtuins: Methods and Protocols presents detailed protocols for sirtuin research that can be followed directly or modified to investigate new areas of sirtuin biology Analysis genen genes genomica genomics diergenetica animal genetics eiwitexpressieanalyse proteomics Molecular Biology (General) Moleculaire biologie (algemeen) Notes Includes index Bibliography Includes bibliographical references and index Notes English Online resource; title from PDF title page (SpringerLink, viewed September 9, 2013) Subject Sirtuins -- Laboratory manuals NAD-ADP-ribosyltransferase. Histone deacetylase. Proteins. Glycosyltransferases. Transferases. Hydrolases. Enzymes. Group III Histone Deacetylases Intracellular Signaling Peptides and Proteins ADP Ribose Transferases Histone Deacetylases Proteins Pentosyltransferases Amino Acids, Peptides, and Proteins Glycosyltransferases Amidohydrolases Transferases Chemicals and Drugs Hydrolases Enzymes Enzymes and Coenzymes Sirtuins protein. enzyme. Transferases Proteins NAD-ADP-ribosyltransferase Hydrolases Histone deacetylase Glycosyltransferases Enzymes Sirtuins Genre/Form Laboratory manuals Laboratory manuals. Manuels de laboratoire. Form Electronic book Author Hirschey, Matthew D., editor ISBN 9781627036375 1627036377 1627036369 9781627036368

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