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Book Cover
E-book
Author Merchán, Miguel A., author

Title Cajal and De Castro's neurohistological methods / by Miguel A. Merchan, Javier de Felipe, and Fernando de Castro
Edition First edition
Published Oxford ; New York : Oxford University Press, [2016]

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Description 1 online resource : illustrations
Contents Cover; Cajal and de Castro's Neurohistological Methods; Copyright; Dedication; Contents; Preface; Foreword; Preliminary Information; List of Plates; Plates; Section One Historical Context; 1 The Authors and Their Backgrounds ; Section Two Basic Histological Techniques; 2 The Microscope and the Fixatives; Microscope; Fixatives; 3 Decalcifying Agents; 4 General Dissociation Procedures; Embedding Techniques and Agents (Celloidin, Paraffin and Gelatin); Dissociation; Sectioning; Frozen Section Procedure; Celloidin and Collodion Embedding; Paraffin Embedding; Gelatin Embedding
Superficial Paraffin Imbedding 5 Handling and Mounting Serial Sections; Sections of Celloidin-​embedded Material; Sections of Paraffin-​embedded Material; 6 General Staining Methods; Some Remarks on the Staining Process; Sources and Composition of Dyes; 7 Formulae for Carmine and Hematoxylin: Substantive and Adjective Coloration with Aniline Stains; Bichromic and Trichromic Methods; Double and Triple Stains; Modifications of the Iron-​Hematoxylin. Methods for the Staining of Mitochondria; 8 Section Mounting and Preservation
Mounting Sections that Require Previous Dehydration and ClarificationSection Mounting without Dehydration and Clarification; Section Three Special Techniques; 9 Methods for the Demonstration of Neuronal Morphology: Golgi's Procedures and Their Variations; 10 Continuation of the Methods for Demonstrating the Morphology of Neurons; Ehrlich's Methylene Blue and its Variations (EN 19); Fixation, Sectioning, and Mounting Techniques for Methylene Blue Stained Material; 11 Methods to Study the Structure of the Nerve Cell; Nissl Chromatic Bodies; Methods for Staining the Neurofibrils
Silver Impregnation MethodsImpregnation of the Golgi Apparatus; Demonstration of Plastosomes, Centrosomes, Oxidases, and Pigmentary Spherules; Nucleus and Cell Membrane; 12 Staining of Neuronal Axons in the Centers; 13 Myelin Staining; 14 Coloration of the Macroglia, Microglia, and Oligodendroglia; Macroglia; Demonstration of the Gliosomes and Mitochondria; Impregnation of Oligodendroglia and Microglia; 15 Methods to Demonstrate the Connective Tissue; Methods for Staining the Connective Tissue; Impregnation Methods for Connective Tissue; Supravital Staining with Acidic Dyes
16 Methods for the Demonstration of Substances Produced by Alterations of the Cell MetabolismDemonstration of Some Cell Granules; Demonstration of Fats and Lipoid Substances; Demonstration of Ferric Pigments; Demonstration of non-​Ferric Pigments; Glycogen Demonstration; Demonstration of Amyloid Substance and Corpora Amylacea; Demonstration of Hyaline Degeneration; Calcareous Substances; 17 Methods for the Demonstration of the Peripheral Nerves under both Normal and Pathological Conditions; Demonstration of Ranvier Nodes; Methods to Stain the Schwann Cells; Methods for Myelin Staining
Summary Cajal and De Castro's Neurohistological Methods provides the first English translation of Fernando de Castro's 1933 publication Elementos de Tecnica Micrografica del Sistema Nervioso. A student of the famed founder of modern neuroscience, with Santiago Ramon y Cajal also serving as the editor of the original text, Fernando de Castro recorded all the various protocols that had been used in his laboratory by his students in order to provide a manual of histological procedures specifically designed for the fine structure of the nervous system
Bibliography Includes bibliographical references and index
Notes Includes translation of "Elementos de Técnica Micrográfica del Sistema Nervioso" (Principles of Micrographical Technique for the Nervous System), originally published in Spanish in 1933
Print version record
Subject Ramón y Cajal, Santiago, 1852-1934
Castro, Fernando de, 1896-1967.
SUBJECT Ramon y Cajal, Santiago, 1852-1934
Castro, Fernando de, 1896-1967
Castro, Fernando de, 1896-1967 fast
Ramón y Cajal, Santiago, 1852-1934 fast
Subject Nervous system.
Histological Techniques -- history
Nervous System -- anatomy & histology
Nervous System
MEDICAL -- Physiology.
SCIENCE -- Life Sciences -- Human Anatomy & Physiology.
Nervous system
Form Electronic book
Author Felipe, Javier de, author
Castro, Fernando de, 1967- author.
Ramón y Cajal, Santiago, 1852-1934. Elementos de Técnica Micrográfica del Sistema Nervioso. English.
LC no. 2015030544
ISBN 9780190221614
0190221615
9780190221621
0190221623