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Title Electron microscopy methods and protocols / edited by M.A. Nasser Hajibagheri
Published Totowa, N.J. : Humana Press, ©1999


Description 1 online resource (xi, 283 pages, 4 pages of plates) : illustrations (some color)
Series Methods in molecular biology ; v. 117
Methods in molecular biology (Clifton, N.J.) ; v. 117
Contents General Preparation of Material and Staining of Sections / Heather A. Davies -- Negative Staining of Thinly Spread Biological Particulates / J. Robin Harris -- Preparation of Thin-Film Frozen-Hydrated/ Vitrified Biological Specimens for Cryoelectron Microscopy / J. Robin Harris and Marc Adrian -- The Production of Cryosections through Fixed and Cryoprotected Biological Material and Their Use in Immunocytochemistry / Paul Webster -- High-Pressure Freezing for Preservation of High Resolution Fine Structure and Antigenicity for Immunolabelling / Kent McDonald -- The Application of LR Gold Resin for Immunogold Labeling / J.R. Thorpe -- Low-Temperature Embedding in Acrylic Resins / Pierre Gounon -- Quantitative Aspects of Immunogold Labeling in Embedded and Nonembedded Sections / Catherine Rabouille -- Microwave Processing Techniques for Electron Microscopy: A Four-Hour Protocol / Rick T. Giberson and Richard S. Demaree, Jr. -- Electron Microscopic Enzyme Cytochemistry / Nobukazu Araki and Tanenori Hatae -- In Situ Molecular Hybridization Techniques for Ultrathin Sections / Jean-Guy Fournier and Francoise Escaig-Haye -- Preparation of the Fission Yeast Schizosaccharomyces pombe for Ultrastructural and Immunocytochemical Study / M.A. Nasser Hajibagheri [and others] -- Preparation of Double/Single-Stranded DNA and RNA Molecules for Electron Microscopy / M.A. Nasser Hajibagheri -- Applications of Electron Microscopy for Studying Protein-DNA Complexes / Maria Schnos and Ross B. Inman -- X-Ray Microanalysis Techniques / A. John Morgan, Carole Winters, and Stephen Sturzenbaum
Summary In Electron Microscopy Methods and Protocols, well-practiced experts describe in detail the key electron microscopy techniques used for examining cells, tissue, biological macromolecules, molecular structure, and their interactions. With emphasis on cryotechniques for quantitative biological X-ray microanalysis, the book also includes those methods that use antibodies to locate proteins within cells and that prepare and analyze nucleic acids, proteins, and protein-nucleic acid complexes. Numerous immunogold labeling techniques for precise ultrastructural localization, distribution, and quantitation of macromolecules in cryo-fixed or chemically-fixed cells are described in sufficient detail to provide practical insight into their advantages and limitations. Electron Microscopy Methods and Protocols offers both newcomers and established researchers wanting to expand their repertoire of cutting-edge electron microscopy techniques-each optimized for reproducibility and robust results-today's gold-standard laboratory manual
Analysis elektronenmicroscopie
electron microscopy
biologische technieken
biological techniques
Biological Techniques
Biologische technieken
Bibliography Includes bibliographical references and index
Notes Master and use copy. Digital master created according to Benchmark for Faithful Digital Reproductions of Monographs and Serials, Version 1. Digital Library Federation, December 2002. MiAaHDL
digitized 2010 HathiTrust Digital Library committed to preserve pda MiAaHDL
Print version record
Subject Electron microscopy -- Laboratory manuals
Histology -- Technique.
Electron microscopy.
Microscopy, Electron
Histocytological Preparation Techniques
Histological Techniques
electron microscopy.
NATURE -- Reference.
SCIENCE -- Life Sciences -- General.
SCIENCE -- Life Sciences -- Biology.
Electron microscopy
Histology -- Technique
Microscopie électronique -- Méthode.
Wetenschappelijke technieken.
Microscopie électronique -- Technique.
Genre/Form Laboratory manuals
Laboratory manuals.
Manuels de laboratoire.
Form Electronic book
Author Hajibagheri, M. A. Nasser
ISBN 0585244006